Ferric uptake regulator mutants of Pseudomonas aeruginosa with distinct alterations in the iron-dependent repression of exotoxin A and siderophores in aerobic and microaerobic environments

Molecular Microbiology

Volumn 21, Issue 5, 1996, Pages 1001-1017

  Barton, Hazel A ^a   Johnson, Zaiga ^a   Cox, Charles D ^b   Vasil, Adriana I ^a   Vasil, Michael L ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXOTOXIN A; FERRIC ION; IRON; SIDEROPHORE; UNCLASSIFIED DRUG;

AEROBIC METABOLISM; ARTICLE; BACTERIAL VIRULENCE; BACTERIUM MUTANT; CONTROLLED STUDY; ENVIRONMENTAL FACTOR; GENE CONTROL; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

EID: 0029798283     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.381426.x     Document Type: Article

References (64)

1. Bagg, A., and Neilands, J.B. (1987) Molecular mechanism of regulation of siderophore mediated iron assimilation. Microbiol Rev 51: 509-518.
2. Berish, S.A., Subbarao, S., Chen, C.Y., Trees, D.L., and Morse, S.A. (1993) Identification and cloning of a fur homolog from Neisseria gonorrhoeae. Infect Immun 61: 4599-4606.
3. Brickman, T.J., and Armstrong, S.K. (1995) Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchi-septica mutants. J Bacteriol 177: 268-270.
4. Brunschwig, E., and Darzins, A. (1992) A two-component T-7 system for the overexpression of genes in P. aeruginosa. Gene 111: 35-41.
5. Chan, V.L., Louie, H., and Bingham, H.L (1995) Cloning and transcription regulation of the ferric uptake regulatory gene of Campylobacter jejuni TGH9011. Gene 164: 25-31.
6. Cox, C.D. (1980) Iron uptake with ferripyochelin and ferric citrate by Pseudomonas aeruginosa. J Bacteriol 142: 581-587.
7. Cox, C.D. (1982) Effect of pyochelin on the virulence of Pseudomonas aeruginosa. Infect Immun 36: 17-23.
8. Coy, M., and Neilands, J.B. (1991) Structural dynamics and functional domains of the Fur protein. Biochemistry 30: 8201-8210.
9. Cunliffe, H.C., Merriman, T.R., and Lamont, I.L. (1995) Cloning and characterization of pvdS, a gene required for pyoverdine synthesis in Pseudomonas aeruginosa: PvdS is probably a member of a family of alternative sigma factors. J Bacteriol 177: 2744-2750.
10. Frank, D.W., and Iglewski, B.H. (1988) Kinetics of toxA and regA mRNA accumulation in Pseudomonas aeruginosa. J Bacteriol 170: 4477-4483.
11. Frank, D.W., Storey, D.G., Hindahl, M.S., and Iglewski, B.H. (1989) Differential regulation by iron of regA and toxA transcript accumulation in Pseudomonas aeruginosa. J Bacteriol 171: 5304-5313.
12. Galimand, M., Gamper, M., Zimmermann, A., and Haas, D. (1991) Positive FNR-like control of anaerobic arginine degradation and nitrate respiration in Pseudomonas aeruginosa. J Bacteriol 173: 1598-1606.
13. Hantke, K. (1981) Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant. Mol Gen Genet 182: 288-292.
14. Hassan, H.M., and Sun, H.C.H. (1992) Regulatory roles of Fnr, Fur, and Arc in expression of manganese-containing Superoxide dismutase of Escherichia coli. Proc Natl Acad Sci USA 89: 3217-3221.
15. Hassett, D.J., Woodruff, W.A., Wozniak, D.J., Vasil, M.L., Cohen, M.S., and Ohman, D.E. (1993) Cloning and characterization of the Pseudomonas aeruginosa sodA and sodB genes encoding manganese- and iron-cofactored Superoxide dismutase: demonstration of increased manganese Superoxide. J Bacteriol 175: 7658-7665.
16. Heinrichs, D.W., and Poole, K. (1993) Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelin and ferripyochelin receptor synthesis in Pseudomonas aeruginosa. J Bacteriol 175: 5882-5889.
17. Hickey, E.K., and Cianciotto, N.P. (1994) Cloning and sequencing of the Legionella pneumophila fur gene. Gene 143: 117-121.
18. Hirakada, Y., Furuya, N., Tateda, K., Kaku, M., and Yamaguchi, K. (1994) In vivo production of exotoxin A and its role in endogenous Pseudomonas aeruginosa septicemia in mice. Infect Immun 61: 2468-2473.
19. Holloway, B.W., and Morgan, A.F. (1986) Genome organization in Pseudomonas. Annu Rev Microbiol 40: 79-106.
20. Holloway, B.W., O'Hoy, K., and Matsumoto, H. (1987) Pseudomonas aeruginosa PAO. In Genetic Maps. O'Brien, S.J. (ed.). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp. 213-221.
21. Karkhoff-Schweizer, R.R., Schryvers, A.B., and Schweizer, H.P. (1994) Cloning and sequence analysis of the fur gene encoding an iron-regulatory protein of Neisseria meningitidis. Gene 141: 139-140.
22. Lam, M.S., Litwin, C.M., Carroll, P.A., and Calderwood, S.B. (1994) Vibrio cholerae fur mutations associated with loss of repressor activity: implications for the structural-functional relationships of Fur. J Bacteriol 176: 5108-5115.
23. Le Cam, E., Frechon, D., Barray, M., Fourcade, A., and Delain, E. (1995) Observation of binding and polymerization of Fur repressor onto operator-containing DNA with electron and atomic force microscopes. Proc Natl Acad Sci USA 91: 11816-11820.
24. Litwin, C.M., and Calderwood, S.B. (1993a) Role of iron in regulation of virulence genes. Clin Microbiol Rev 6: 137-149.
25. Litwin, C.M., and Calderwood, S.B. (1993b) Cloning and genetic analysis of the Vibrio vulnificus fur gene and construction of a fur mutant by in vivo marker exchange. J Bacteriol 175: 706-15.
26. Litwin, C.M., Boyko, S.A., and Calderwood, S.B. (1992) Cloning, sequencing, and transcriptional regulation of the Vibrio cholerae fur gene. J Bacteriol 174: 1897-1903.
27. deLorenzo, V., Giovannini, F., Herrero, M., and Neilands, J.B. (1988a) Metal ion regulation of gene expression. Fur repressor-operator interaction at the promoter region of the aerobactin system of pColV-K30. J Mol Biol 203: 875-884.
28. deLorenzo, V., Herrero, M., Giovannini, F., and Neilands, J.B. (1988b) Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of fur gene in Escherichia coli. Eur J Biochem 173: 537-546.
29. Nees, D.W., Stein, P.A., and Ludwig, R.A. (1988) The Azorhizobium caulinodans nifA gene: identification of upstream-activating sequences including a new element, the 'anaerobox'. Nucl Acids Res 16: 9839-9853.
30. Neilands, J.B. (1990) Ferric uptake regulation (Fur) repressor: facts and fantasies. In Pseudomonas Biotransformations, Pathogenesis and Evolving Biotechnology. Silver, S.C., Iglewski, A.M., and Kaplan, S. (eds). Washington, DC: American Society for Microbiology, pp. 382-395.
31. Niederhoffer, E.C., Naranjo, C.M., Bradley, K.L., and Fee, J.A. (1990) Control of Escherichia coli Superoxide dismutase (sodA and sodB) genes by the ferric uptake regulation (fur) locus. J Bacteriol 172: 1930-1938.
32. Ochsner, U.A., and Vasil, M.L. (1996) Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes. Proc Natl Acad Sci USA 93: 4409-4414.
33. Ochsner, U.A., Vasil, A.I., and Vasil, M.L. (1995) Role of the ferric uptake regulator of Pseudomonas aeruginosa in the regulation of siderophores and exotoxin A expression: purification and activity on iron-regulated promoters. J Bacteriol 177: 7194-7201.
34. Ochsner, U.A., Johnson, Z., Lamont, I.L., Cunliffe, H.E., and Vasil, M.L. (1996) Exotoxin A production in Pseudomonas aeruginosa requires the iron-regulated pvdS gene encoding an alternative sigma factor. Mol Microbiol 21: 1001-1010.
35. Poole, K., Neshat, S., and Heinrichs, D. (1991) Pyoverdine-mediated iron transport in Pseudomonas aeruginosa: involvement of a high-molecular-mass outer membrane protein. FEMS Microbiol Lett 78: 1-5.
36. Prince, R.W., Storey, D.G., Vasil, A.I., and Vasil, M.L. (1991) Regulation of toxA and regA by the Escherichia coli fur gene and identification of a Fur homologue in Pseudomonas aeruginosa PA103 and PA01. Mol Microbiol 5: 2823-2831.
37. Prince, R.W., Cox, C.D., and Vasil, M.L. (1993) Coordinate regulation of siderophore and exotoxin A production: molecular cloning and sequencing of the Pseudomonas aeruginosa fur gene. J Bacteriol 175: 2589-2598.
38. Rahme, L.G., Stevens, E.J., Wolfort, S.F., Shao, J., Tomp-kins, R.G., and Ausubel, F.M. (1995) Common virulence factors for bacterial pathogenicity in plants and animals. Science 268: 1899-1902.
39. Saito, T., and Williams, R.J.P. (1991) The binding of the ferric uptake regulation protein to a DNA fragment. Eur J Biochem 197: 43-47.
40. Saito, T., Duly, D., and Williams, R.J. (1991a) The histidines of the iron-uptake regulation protein, Fur. Eur J Biochem 197: 39-42.
41. Saito, T., Wormald, M.R., and Williams, R.J.P. (1991b) Some structural features of the iron-uptake regulation protein. Eur J Biochem 197: 29-38.
42. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Habor Laboratory Press.
43. Sawers, R.G. (1991) Identification and molecular characterization of a transcriptional regulator from Pseudomonas aeruginosa PA01 exhibiting structural and functional similarity to the FNR protein of Escherichia coli. Mol Microbiol 5: 1469-1481.
44. Schwyn, B., and Neilands, J.B. (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160: 47-56.
45. Simon, R., Priefer, U., and Pühler, A. (1983) A broad-host-range mobilizable system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1: 784-791.
46. Spiro, S., and Guest, J.R. (1990) FNR and its role in oxygen-regulated gene expression in Escherichia coli. FEMS Microbiol Rev 75: 399-428.
47. Staggs, T.M., and Perry, R.D. (1991) Identification and cloning of a fur regulatory gene in Yersinia pestis. J Bacteriol 173: 417-425.
48. Storey, D.G., Frank, D.W., Farinha, M.A., Kropinski, A.M., and Iglewski, B.H. (1990) Multiple promoters control the regulation of the Pseudomonas aeruginosa regA gene. Mol Microbiol 4: 499-503.
49. Storey, O.G., Raivio, T.L., Frank, D.W., Wick, M.J., Kaye, S., and Iglewski, B.H. (1991) Effect of regB on expression from the P1 and P2 promoters of the Pseudomonas aeruginosa regAB operon. J Bacteriol 173: 6088-6094.
50. Tardat, B., and Touati, D. (1991) Two global regulators repress the anaerobic expression of MnSOD in Escherichia coli::Fur (ferric uptake regulation) and Arc (aerobic respiration control). Mol Microbiol 5: 455-465.
51. Tardat, B., and Touati, D. (1993) Iron and oxygen regulation of Escherichia coli MnSOD expression: competition between the global regulators Fur and ArcA for binding to DNA. Mol Microbiol 9: 53-63.
52. Tolmasky, M.E., Wertheimer, A.M., Actis, L.A., and Crosa, J.H. (1994) Characterization of the Vibrio anguillarum fur gene: role in regulation of expression of the FatA outer membrane protein and catechols. J Bacteriol 176: 213-220.
53. Touati, D., Jacques, M., Tardat, B., Bouchard, L., and Despied, S. (1995) Lethan oxidative damage and mutagenesis are generated by iron in Dfur mutants of E. coli: protective role of Superoxide dismutase. J Bacteriol 177: 2305-2314.
54. Vander Wauven, C., Piérard, A., Klay-Raymann, M., and Haas, D. (1984) Pseudomonas aeruginosa mutants affected in anaerobic growth on arginine: evidence for a fourgene cluster encoding the arginine deiminase pathway. J Bacteriol 160: 928-934.
55. Vasil, M.L. (1986) Pseudomonas aeruginosa: biology, mechanisms of virulence, epidemiology. J Pediatr 108: 800-805.
56. Vasil, M.L., Chamberlain, C., and Grant, C.C.R. (1986) Molecular studies of Pseudomonas exotoxin A gene. Infect Immun 52: 538-548.
57. Vasil, M.L., Grant, C.C.R., and Prince, R.W. (1989) Regulation of exotoxin A synthesis in Pseudomonas aeruginosa: characterization of toxA-lacZ fusions in wild-type and mutant strains. Mol Microbiol 3: 371-381.
58. Venturi, V., Ottevanger, C., Bracke, M., and Weisbeek, P. (1995) Iron regulation of siderophore biosynthesis and transport in Pseudomonas putida WCS358: involvement of a transcriptional activator and of the Fur protein. Mol Microbiol 15: 1081-1093.
59. Wertheimer, A., Tolmasky, M.E., Actis, L.A., and Crosa, J.H. (1994) Structural and functional analyses of mutant Fur proteins with impaired regulatory function. J Bacteriol 176: 5116-5122.
60. Wick, M.J., Frank, D.W., Storey, D.G., and Iglewski, B.H. (1990) Structure, function, and regulation of Pseudomonas aeruginosa exotoxin A. Annu Rev Microbiol 44: 335-363.
61. Wooldridge, K.G., Williams, P.H., and Ketley, J.M. (1994) Iron-responsive genetic regulation in Campylobacter jejuni: cloning and characterization of a fur homolog. J Bacteriol 176: 5852-5856.
62. Ye, R.W., Haas, D., Ka, J.-O., Krishnapillai, V., Zimmerman, A., Baird, C., and Tiedje, J.M. (1995) Anaerobic activation of the entire denitrification pathway in Pseudomonas aeruginosa requires Anr, an analog of Fnr. J Bacteriol 177: 3606-3609.
63. Ziegelhoffer, E.C., and Kiley, P.J. (1995) In vitro analysis of a constitutively active mutant form of the Escherichia coli global transcription factor FNR. J Mol Biol 245: 351-361.
64. Zimmermann, A., Reimmann, C., Galimand, M., and Haas, D. (1991) Anaerobic growth and cyanide synthesis of Pseudomonas aeruginosa depend on anr, a regulatory gene homologous with fnr of Escherichia coli. Mol Microbiol 5: 1483-1490.