Isolation and characterization of a Pseudomonas aeruginosa gene, ptxR, which positively regulates exotoxin A production

Molecular Microbiology

Volumn 21, Issue 1, 1996, Pages 97-110

  Hamood, Abdul N ^a   Colmer, Jane A ^a   Ochsner, Urs A ^b   Vasil, Michael L ^b  

^a TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXOTOXIN A; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; GENETIC REGULATION; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; TOXIN SYNTHESIS;

EID: 0030016793     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.6251337.x     Document Type: Article

References (72)

1. Ausubel, F., Brent, R., Kingston, R., Moor, D., Seidman, J., Smith, J., and Strauhle, K. (1988) Current Protocols in Molecular Biology. New York: Wiley Inter-sciences.
2. Bagdasarian, M., and Timmis K. (1982) Host:vector system for gene cloning in Pseudomonas. Curr Top Microbiol Immunol 96: 47-67.
3. Bjorn, M.J., Iglewski, B.H., Ives, S.K., Sadoff, U.C., and Vasil, M.L. (1978) Effect of iron on yields of exotoxin A in cultures of Pseudomonas aeruginosa PA-103. Infect Immun 19: 785-791.
4. Boyer, H.W., and Rolland-Dussoix, D. (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41: 459-472.
5. Brumbley, S., Carney, B., and Denny, T. (1993) Phenotypic conversion in Pseudomonas solanacearum due to spontaneous inactivation of PhcA, a putative LysR transcriptional regulator. J Bacteriol 175: 54477-5487.
6. Brunschwig, E., and Darzins, A. (1992) A two-component T7 system for the overexpression of genes in Pseudomonas aeruginosa. Gene 111: 35-41.
7. Chang, M., and Crawford, I. (1990) The roles of indoleglycerol phosphate and the Trpl protein in the expression of trpBA from Pseudomonas aeruginosa. Nucl Acids Res 18: 979-987.
8. Chou, P., and Fasman, G.D. (1974) Prediction of protein conformation. Biochemistry 13: 222-224.
9. Christman, M.F., Storz, G., and Ames, B.N. (1989) OxyR, a positive regulator of hydrogen peroxide-inducible genes in Escherichia coli and Salmonella typhimurium homologous to a family of bacterial regulatory proteins. Biochemistry 86: 3484-3488.
10. Crosa, J.H. (1989) Genetic and molecular biology of siderophore-mediated iron transport in bacteria. Microbiol Rev 53: 517-530.
11. Cunliffe, H.C., Merriman, T.R., and Lamont, I.L. (1995) Cloning and characterization of pvdS, a gene required for pyoveridine synthesis in Pseudomonas aeruginosa: PvdS is a member of a family of alternative sigma factors. J Bacteriol 177: 2744-2750.
12. Devereux, J., Haeberle, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387-395.
13. Ditta, G., Starfield, S., Corbin, D., and Helinski, D.R. (1980) Broad host range DNA cloning system for gram negative bacteria. Construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347-7351.
14. Dodd, I., and Egan, J. (1987) Systematic method for the detection of potential lambda Cro-like DNA binding regions in proteins. J Biol Chem 194: 557-564.
15. Frank, D.W., and Iglewski, B.H. (1988) Kinetic of toxA and regA mRNA accumulation in Pseudomonas aeruginosa. J Bacteriol 170: 4477-4483.
16. Frank, D.W., Storey, D.G., Hindahl, M.S., and B.H. Iglewski, B.H. (1989) Differential regulation by iron of toxA and regA transcripts accumulation in Pseudomonas aeruginosa. J Bacteriol 171: 5304-5313.
17. Friedman, A.M., Long, S.R., Brown, S.E., Buikkema, W.J., and Ausubel, P.M. (1982) Construction of a broad host range cosmid cloning vector and its usage in the genetic analysis of Rhizobium mutants. Gene 18: 289-296.
18. Gambello, M.J., and Iglewski, B.H. (1991) Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J Bacteriol 173: 3000-3009.
19. Gambello, M.J., Kaye, S., and Iglewski, B.H. (1993) IasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and enhancer of exotoxin A expression. Infect Immun 61: 1180-1184.
20. Goethals, K., Montagu, M., and Holsters, M. (1992) Conserved motifs in a divergent nod box of Azorhizobium caulinodans ORS571 reveals a common structure in promoters regulated by the LysR-type proteins. Proc Natl Acad Sci USA 89: 1646-1650.
21. Goldberg, J.B., and Ohman, D.E. (1984) Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate. J Bacteriol 158: 1115-1121.
22. Goldberg, M.B., Boyko, S.A., and Calderwood, S.B. (1991) Positive transcriptional regulation of an iron-regulated virulence gene in Vibrio cholerae. Proc Natl Acad Sci USA 88: 1125-1129.
23. Grant, C.R., and Vasil, M.L. (1986) Analysis of transcription of the exotoxin A gene of Pseudomonas aeruginosa. J Bacteriol 168: 1112-1119.
24. Gray, G., and Vasil, M.L. (1981) Isolation and genetic characterization of exotoxin A deficient mutants of Pseudomonas aeruginosa PAO1. J Bacteriol 147: 275-281.
25. Hamood, A., and Iglewski, B.H. (1990) Expression of the Pseudomonas aeruginosa toxA positive revelatory gene (regA) in Escherichia coli. J Bacteriol 172: 589-594.
26. Hamood, A.M., Olson, J.C., Vincent, T., and Iglewski, B.H. (1989) Regions of exotoxin A involved in exotoxin A excretion in Pseudomonas aeruginosa J Bacteriol 171: 1817-1824.
27. Hamood, A.N., Ohman, D.E., West, S.E., and Iglewski, B.H. (1992) Characterization of exotoxin A excretion deficient mutants of the Pseudomonas aeruginosa strain PAO1. Infect Immun 60: 510-517.
28. Hedstrom, R.C., Funk, C.R., Kaper, J.B., and Galloway, D.R. (1986) Cloning of a gene involved in regulation of exotoxin A expression in Pseudomonas aeruginosa. Infect Immun 51: 37-42.
29. Henikoff, S., Haughn, G., Calvo, J., and Wallace, J. (1988) A large family of bacterial activator proteins. Proc Natl Acad Sci USA 85: 6602-6606.
30. Hindahl, M., Frank, D.W., and Iglewski, B.H. (1987) Molecular studies of a positive regulator of exotoxin A synthesis in Pseudomonas aeruginosa. Antibiot Chemother 39: 279-289.
31. Hindahl, M., Frank, D.W., Hamood, A., and Iglewski, B.H. (1988) Characterization of a gene that regulates exotoxin A synthesis in Pseudomonas aeruginosa. Nucl Acids Res 16: 5699, correction 8725.
32. Holloway, B.W., Krishnapillai, V., and Morgan, A.F. (1979) Chromosomal genetics of Pseudomonas. Microbiol Rev 43: 73-102.
33. Holloway, B.W., Romling, U., and Tummler, B. (1994) Genomic mapping of Pseudomonas aeruginosa PAO. Microbiology 140: 2907-2929.
34. Iglewski, B.H., and Kabat, D. (1975) NAD dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin. Proc Acad Sci USA 72: 2284-2288.
35. Liu, P.V. (1966) The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis. III. Identity of the lethal toxins produced in vivo. J Inf Dis 16: 481-489.
36. Liu, P. (1973) Exotoxins of Pseudomonas aeruginosa. I. Factors that influence the production of exotoxin A. J Inf Dis 128: 506-513.
37. Liu, P. (1974) Extracellular toxins of Pseudomonas aeruginosa. J Inf Dis 130 (Suppl): S94-S99.
38. Lory, S. (1986) Effect of iron on accumulation of exotoxin A specific mRNA in Pseudomonas aeruginosa. J Bacteriol 168: 1451-1456.
39. Miller, J.H. (1972) Experiments in Molecular Genetics, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
40. Neumann, J.R., Morency, C.A., and Russian, K.O. (1987) A novel rapid assay for chloramphenicol acetyltransferase gene expression. Biotechniques 5: 444-447.
41. Ochsner, U.A., Vasil, A.I., and Vasil, M.L. (1995) Role of the ferric uptake regulator of Pseudomonas aeruginosa in the regulation of siderophores and exotoxin A expression: purification and activity of iron-regulated promoters. J Bacteriol 177: 7194-7201.
42. Ohman, D.E., Sadoff, J.C., and Iglewski, B.H. (1980) Exotoxin A deficient mutants of Pseudomonas aeruginosa PA103: isolation and characterization. Infect Immun 28: 899-908.
43. Olson, R.H., Debusscher, G., and McCombie, W.R. (1982) Development of broad host range vectors and gene banks: self cloning of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol 150: 60-69.
44. Ostrowiski, J., Jagura-Burdzy, G., and Kredich, N.M. (1987) DNA sequence of the cysB regions of Salmonella typhimurium and Escherichia coli. J Biol Chem 262: 5999-6005.
45. Pabo, C.O., and Sauer, R. (1984) Protein-DNA recognition. Annu Rev Biochem 53: 293-321.
46. Prince R.W., Storey, D.G., Vasil, A.I., and Vasil, M.L. (1991) Regulation of toxA and regA by the Escherichia coli fur gene and identification of a fur homologue in Pseudomonas aeruginosa PA103 and PAO1. Mol Microbiol 5: 2828-2831.
47. Prince, R.W., Cox, C.D., and Vasil M.L. (1993) Coordinate regulation of siderophore and exotoxin A production: molecular cloning and sequencing of the Pseudomonas aeruginosa fur gene. J Bacteriol 175: 2589-2598.
48. Rothmal, R.K., Aldrich, T.L., Houghton, J., Coco, W., Ornston, L.N., and Chakrabarty, A.M. (1990) Nucleotide sequencing and characterization of Pseudomonas putida catR: a positive regulator of the catBC operon is a member of the LysR family. J Bacteriol 172: 922-931.
49. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
50. Schwyn, B., and Neilands, J.B. (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160: 47-56.
51. Shortridge, V.D., Pato, M.L., Vasil, A.I., and Vasil, M.L. (1991) Physical mapping of virulence-associated genes in Pseudomonas aeruginosa by transverse alternating-field electrophoresis. Infect Immun 59: 3596-3603.
52. Silhavy, T.J., Berman, M.L., and Enquist, L.W. (1984) Experiments with Gene Fusion. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
53. Smith, A., and Iglewski, B.H. (1989) Transformation of Pseudomonas aeruginosa by electroporation. Nucl Acids Res 17: 10509.
54. Stibitz, S., Black, W., and Falkow, S. (1986) The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50: 133-140.
55. Storey, D., Frank, D.W., Farinha, M., Kropinski, A., and Iglewski, B.H. (1990) Multiple promoters control the regulation of the Pseudomonas aeruginosa regA gene. Mol Microbiol 4: 499-504.
56. Storey, D., Raivio, T., Frank, D., Wick, M.-J., Kaye, S., and Iglewski, B.H. (1991) Effect of regB on expression from the P1 and P2 promoters of the Pseudomonas aeruginosa regAB operon. J Bacteriol 173: 6088-6094.
57. Tabor, S., and Richardson, C. (1985) A bacteriophage T7 RNA polymerase/promoters system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82: 1074-1078.
58. Van der Meer, J.R., Eggan., R.I., Zehnder, B., and De Vos, W.M. (1991) Sequence analysis of the Pseudomonas sp. strain p51 tbc gene cluster, which encodes metabolism of chlorinated catecholes. J Bacteriol 173: 2525-2434.
59. Vasil, M.L., Kabat, D., and Iglewski, B.H. (1977) Structure-activity relationships of an exotoxin of Pseudomonas aeruginosa. Infect Immun 16: 353-361.
60. Vasil, M.L., Grant, C.R., and Prince, R.W. (1989) Regulation of exotoxin A synthesis in Pseudomonas aeruginosa: characterization of toxA-lacZ fusions in wild-type and mutant strains. Mol Microbiol 3: 371-381.
61. Viale, A., Koboyashi, H., Akazawa, T., and Heinikoff, S. (1991) rbcR, a gene coding for member of the LysR family of transcriptional regulators, is located upstream of the expressed set of ribulose 1,5-bisphosphate carboxylase/ oxygenase genes in the photosynthetic bacterium Chromatium vinosum. J Bacteriol 173: 5224-5229.
62. Von Heijne, G. (1985) Signal sequences. The limit of variation. J Mol Biol 184: 99-105.
63. Walker, S.L., Hiremath, L., Wozniak, D., and Galloway, D. (1995) ToxR (RegA) activates Escherichia coli RNA polymerase to initiate transcription of Pseudomonas aeruginosa toxA. Gene 154: 15-21.
64. West, S.E., and Iglewski, B.H. (1988) Codon usage in Pseudomonas aeruginosa. Nucl Acids Res 16: 9323-9335.
65. West, S.E., Storey, D.G., and Iglewski, B.H. (1989) Characterization of Pseudomonas aeruginosa exotoxin A mutants. Abstracts of the Annual Meeting of the American Society for Microbiology, New Orleans, Louisiana. Abstract B-231, p. 69.
66. West, S.E., Sample, A., and Runyen-Janecky, L. (1994a) The vfr gene product, required for Pseudomonas aeruginosa exotoxin A and protease production, belongs to the cyclic AMP receptor protein family. J Bacteriol 176: 7532-7542.
67. West, S.E., Kaye, S., Hamood, A., and Iglewski, B.H. (1994b) Characterization of Pseudomonas aeruginosa mutants that are deficient in exotoxin A synthesis and are altered in expression of regA, a positive regulator of exotoxin A. Infect Immun 62: 897-903.
68. Wick, M.J., Frank, D.W., Story, D., and Iglewski, B.H. (1990a) Identification of regB, a gene required for optimal exotoxin A yields in Pseudomonas aeruginosa. Mol Microbiol 4: 489-498.
69. Wick, M.J., Frank, D.W., Story, D., and Iglewski, B.H. (1990b) Structure, function, and regulation of Pseudomonas aeruginosa exotoxin A. Annu Rev Microbiol 44: 335-363.
70. Wozniak, D.J., Daniels, D.C., and Galloway, D.R. (1987) Nucleotide sequence and characterization of toxR: a gene involved in exotoxin A regulation in Pseudomonas aeruginosa. Nucl Acids Res 15: 2123-2135.
71. Yanisch-Perron, C., Vieira, C.J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and M13mp19 vectors. Gene 33: 103-119.
72. Zimniak, L., Dyan, A., and Iglewski, B.H. (1989) Identification of RegA protein from Pseudomonas aeruginosa using anti-RegA antibody. Biochem Biophys Res Comm 163: 1312-1318.