Fluorescent target array killing assay: A multiplex cytotoxic T-cell assay to measure detailed T-cell antigen specificity and avidity in vivo

Cytometry Part A

Volumn 81 A, Issue 8, 2012, Pages 679-690

  Quah, Benjamin J C ^a   Wijesundara, Danushka K ^a   Ranasinghe, Charani ^a   Parish, Christopher R ^a  

^a Australian National University   (Australia)

Author keywords

Cytotoxic T cell killing assay; Flow cytometry; Multiplex

Indexed keywords

EPITOPE; FLUORESCENT DYE; HUMAN IMMUNODEFICIENCY VIRUS VACCINE;

ANIMAL; ANTIBODY AFFINITY; ARTICLE; BIOASSAY; CELL DEATH; CYTOLOGY; CYTOTOXIC T LYMPHOCYTE; FLUORESCENCE; IMMUNOLOGY; METABOLISM; METHODOLOGY; MOUSE; SPLEEN;

AIDS VACCINES; ANIMALS; ANTIBODY AFFINITY; BIOLOGICAL ASSAY; CELL DEATH; EPITOPES; FLUORESCENCE; FLUORESCENT DYES; MICE; SPLEEN; T-LYMPHOCYTES, CYTOTOXIC;

EID: 84864008841     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22084     Document Type: Article

References (31)

1. Lieberman J. The ABCs of granule-mediated cytotoxicity: New weapons in the arsenal. Nat Rev Immunol 2003; 3: 361-370.
2. Chan FK, Chun HJ, Zheng L, Siegel RM, Bui KL, Lenardo MJ. A domain in TNF receptors that mediates ligand-independent receptor assembly and signaling. Science 2000; 288: 2351-2354.
3. Zaritskaya L, Shurin MR, Sayers TJ, Malyguine AM. New flow cytometric assays for monitoring cell-mediated cytotoxicity. Expert Rev Vaccines 2010; 9: 601-616.
4. Brunner KT, Mauel J, Cerottini JC, Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology 1968; 14: 181-196.
5. Karawajew L, Jung G, Wolf H, Micheel B, Ganzel K. A flow cytometric long-term cytotoxicity assay. J Immunol Methods 1994; 177: 119-130.
6. Oehen S, Brduscha-Riem K. Differentiation of naive CTL to effector and memory CTL: Correlation of effector function with phenotype and cell division. J Immunol 1998; 161: 5338-5346.
7. Flieger D, Gruber R, Schlimok G, Reiter C, Pantel K, Riethmuller G. A novel non-radioactive cellular cytotoxicity test based on the differential assessment of living and killed target and effector cells. J Immunol Methods 1995; 180: 1-13.
8. Jerome KR, Sloan DD, Aubert M. Measurement of CTL-induced cytotoxicity: The caspase 3 assay. Apoptosis 2003; 8: 563-571.
9. Goldberg JE, Sherwood SW, Clayberger C. A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry. J Immunol Methods 1999; 224: 1-9.
10. Hermans IF, Silk JD, Yang J, Palmowski MJ, Gileadi U, McCarthy C, Salio M, Ronchese F, Cerundolo V. The VITAL assay: A versatile fluorometric technique for assessing CTL- and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo. J Immunol Methods 2004; 285: 25-40.
11. Weston SA, Parish CR. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J Immunol Methods 1990; 133: 87-97.
12. Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. JImmunol Methods 1994; 171: 131-137.
13. Parish CR Fluorescent dyes for lymphocyte migration and proliferation studies. Immunol Cell Biol 1999; 77: 499-508.
14. Quah BJ, Warren HS, Parish CR. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nat Protoc 2007; 2: 2049-2056.
15. Quah BJ, Parish CR. New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes. J Immunol Methods 2012; 379: 1-14.
16. Krutzik PO, Nolan GP. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nat Methods 2006; 3: 361-368.
17. Hogquist KA, Jameson SC, Heath WR, Howard JL, Bevan MJ, Carbone FR. T cell receptor antagonist peptides induce positive selection. Cell 1994; 76: 17-27.
18. Pircher H, Burki K, Lang R, Hengartner H, Zinkernagel RM. Tolerance induction in double specific T-cell receptor transgenic mice varies with antigen. Nature 1989; 342: 559-561.
19. Mamalaki C, Elliott J, Norton T, Yannoutsos N, Townsend AR, Chandler P, Simpson E, Kioussis D. Positive and negative selection in transgenic mice expressing a T-cell receptor specific for influenza nucleoprotein and endogenous superantigen. Dev Immunol 1993; 3: 159-174.
20. Quah B, Ni K, O'Neill HC. In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity. Int Immunol 2004; 16: 567-577.
21. Ranasinghe C, Medveczky JC, Woltring D, Gao K, Thomson S, Coupar BE, Boyle DB, Ramsay AJ, Ramshaw IA. Evaluation of fowlpox-vaccinia virus prime-boost vaccine strategies for high-level mucosal and systemic immunity against HIV-1. Vaccine 2006; 24: 5881-5895.
22. Quah BJ, Parish CR. The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation. J Vis Exp 2010; 44: e2259.
23. Betts MR, Brenchley JM, Price DA, de Rosa SC, Douek DC, Roederer M, Koup RA. Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J Immunol Methods 2003; 281: 65-78.
24. Wang B, Sharma A, Maile R, Saad M, Collins EJ, Frelinger JA. Peptidic termini play a significant role in TCR recognition. J Immunol 2002; 169: 3137-3145.
25. Ranasinghe C, Eyers F, Stambas J, Boyle DB, Ramshaw IA, Ramsay AJ. A comparative analysis of HIV-specific mucosal/systemic T cell immunity and avidity following rDNA/rFPV and poxvirus-poxvirus prime boost immunisations. Vaccine 2011; 29: 3008-3020.
26. Alexander-Miller MA, Leggatt GR, Sarin A, Berzofsky JA. Role of antigen, CD8, and cytotoxic T lymphocyte (CTL) avidity in high dose antigen induction of apoptosis of effector CTL. J Exp Med 1996; 184: 485-492.
27. La Gruta NL, Turner SJ, Doherty PC. Hierarchies in cytokine expression profiles for acute and resolving influenza virus-specific CD8+ T cell responses: Correlation of cytokine profile and TCR avidity. J Immunol 2004; 172: 5553-5560.
28. Alexander-Miller MA. High-avidity CD8+ T cells: Optimal soldiers in the war against viruses and tumors. Immunol Res 2005; 31: 13-24.
29. Ranasinghe C, Turner SJ, McArthur C, Sutherland DB, Kim JH, Doherty PC, Ramshaw IA. Mucosal HIV-1 pox virus prime-boost immunization induces high-avidity CD8+ T cells with regime-dependent cytokine/granzyme B profiles. J Immunol 2007; 178: 2370-2379.
30. Gilbert DF, Wilson JC, Nink V, Lynch JW, Osborne GW. Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry. Cytometry. Part A: J Int Soc Anal Cytol 2009; 75: 440-449.
31. Parish CR, Glidden MH, Quah BJ, Warren HS. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol 2009; 84: 4.9.1-4.9.13.