A high-throughput, multiplexed kinase assay using a benchtop orbitrap mass spectrometer to investigate the effect of kinase inhibitors on kinase signaling pathways

Analytical Chemistry

Volumn 84, Issue 14, 2012, Pages 6233-6239

  Kunz, Ryan C ^a   McAllister, Fiona E ^a   Rush, John ^b   Gygi, Steven P ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b CELL SIGNALING TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

96-WELL PLATE; CELL LYSATES; DATA QUALITY; HIGH-THROUGHPUT; HIGH-THROUGHPUT METHOD; IMMUNE RESPONSE; KINASE ACTIVITY; KINASE ASSAYS; KINASE INHIBITORS; ORBITRAP; PEPTIDE SUBSTRATES; PHOSPHOPEPTIDE ENRICHMENT; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN PHOSPHORYLATION; REACTION MIXTURE; SIGNALING PATHWAYS; SUBSTRATE PEPTIDES;

DESALINATION; ENZYME ACTIVITY; MASS SPECTROMETERS; PHOSPHORYLATION; SUBSTRATES;

PEPTIDES;

PEPTIDE; PROTEIN KINASE; PROTEIN KINASE INHIBITOR;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COMPUTER PROGRAM; DRUG EFFECT; ENZYME ASSAY; HUMAN; IC 50; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; SIGNAL TRANSDUCTION; TUMOR CELL LINE;

AMINO ACID SEQUENCE; CELL LINE, TUMOR; ENZYME ASSAYS; HUMANS; INHIBITORY CONCENTRATION 50; MASS SPECTROMETRY; PEPTIDES; PROTEIN KINASE INHIBITORS; PROTEIN KINASES; SIGNAL TRANSDUCTION; SOFTWARE;

EID: 84863962527     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac301116z     Document Type: Article

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