Constitutive expression of barley α-amylase in Pichia pastoris by high-density cell culture

Molecular Biology Reports

Volumn 39, Issue 5, 2012, Pages 5805-5810

  Liu, Z W ^a   Yin, H X ^a   Yi, X P ^a   Zhang, A L ^a   Luo, J X ^b   Zhang, T Y ^b   Fu, C Y ^c   Zhang, Z H ^a   Shen, J C ^a   Chen, L P ^a  

^a INSTITUTE OF TROPICAL BIOSCIENCE AND BIOTECHNOLOGY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

^c Hainan Provincial Institute for Drug Control   (China)

Author keywords

Amylase; Bioactivity; Constitutive expression; High density cell culture; Pichia pastoris

Indexed keywords

AMYLASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCEROL; PEPTONE; SEPHAROSE; STARCH; RECOMBINANT PROTEIN;

ALPHA AMYLASE GENE; ARTICLE; BARLEY; BIOREACTOR; CONTROLLED STUDY; ELECTROPORATION; FERMENTATION TECHNIQUE; FUNGAL BIOMASS; FUNGAL CELL CULTURE; FUNGAL STRAIN; GENE AMPLIFICATION; GENE DOSAGE; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE FUNCTION; GENETIC SCREENING; GENETIC TRANSFORMATION; HETEROZYGOTE; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PLANT GENE; QUANTITATIVE ASSAY; RECOMBINANT GENE; TEMPERATURE DEPENDENCE; BIOMASS; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; ENZYMOLOGY; FERMENTATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HORDEUM; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

HORDEUM; PICHIA PASTORIS;

ALPHA-AMYLASES; BIOMASS; CELL CULTURE TECHNIQUES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; GENE DOSAGE; HORDEUM; HYDROLYSIS; PEPTONES; PICHIA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; STARCH; TRANSFORMATION, GENETIC;

EID: 84863752654     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-1390-1     Document Type: Article

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