Fluorophore labeled kinase detects ligands that bind within the MAPK insert of p38α kinase

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Getlik, Matthäus ^a,d   Simard, Jeffrey R ^a,d   Termathe, Martin ^a,d   Grütter, Christian ^a,b,d   Rabiller, Matthias ^a,d   van Otterlo, Willem A L ^c   Rauh, Daniel ^a,b,d  

^a CHEMICAL GENOMICS CENTRE OF THE MAX PLANCK SOCIETY   (Germany)

^b TU DORTMUND UNIVERSITY   (Germany)

^c STELLENBOSCH UNIVERSITY   (South Africa)

^d MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE 14; FLUORESCENT DYE; LIGAND;

AFFINITY LABELING; ARTICLE; BINDING AFFINITY; BINDING ASSAY; BINDING SITE; CONCENTRATION (PARAMETERS); CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME ASSAY; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SEQUENCING; LIGAND BINDING; MOLECULAR EVOLUTION; MOLECULAR INTERACTION; MOLECULAR LIBRARY; MOLECULAR MODEL; PROTEIN FUNCTION; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; BIOASSAY; CHEMISTRY; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PROTEIN TERTIARY STRUCTURE;

BINDING SITES; BIOLOGICAL ASSAY; FLUORESCENT DYES; HUMANS; LIGANDS; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 14; PROTEIN STRUCTURE, TERTIARY;

EID: 84863634917     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0039713     Document Type: Article

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