Targeting FAK radiosensitizes 3-dimensional grown human HNSCC cells through reduced akt1 and MEK1/2 signaling

International Journal of Radiation Oncology Biology Physics

Volumn 83, Issue 5, 2012, Pages

  Hehlgans, Stephanie ^a,b,c   Eke, Iris ^a   Cordes, Nils ^a,c  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b GOETHE UNIVERSITY   (Germany)

^c INSTITUTE OF ION BEAM PHYSICS AND MATERIALS RESEARCH   (Germany)

Author keywords

FAK; Focal adhesion kinase; Ionizing radiation; MEK1 2; Squamous cell carcinoma; Three dimensional cell culture

Indexed keywords

FAK; FOCAL ADHESION KINASE; MEK1/2; SQUAMOUS CELL CARCINOMA; THREE DIMENSIONAL CELL CULTURE;

ADHESION; CELL CULTURE; CELLS; ENZYMES; IONIZING RADIATION; PHOSPHORYLATION; RADIOTHERAPY;

SIGNALING;

1,4 DIAMINO 1,4 BIS(2 AMINOPHENYLTHIO) 2,3 DICYANOBUTADIENE; FOCAL ADHESION KINASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE 2; PAXILLIN; PROTEIN KINASE B; SMALL INTERFERING RNA;

ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CELL SURVIVAL; CLONOGENESIS; CONTROLLED STUDY; EXTRACELLULAR MATRIX; HEAD AND NECK SQUAMOUS CELL CARCINOMA; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN EXPRESSION; PROTEIN TARGETING; RADIOSENSITIVITY; RADIOSENSITIZATION; SIGNAL TRANSDUCTION;

BUTADIENES; CARCINOMA, SQUAMOUS CELL; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL SURVIVAL; FOCAL ADHESION KINASE 1; HEAD AND NECK NEOPLASMS; HUMANS; MAP KINASE KINASE KINASE 1; MAP KINASE KINASE KINASE 2; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MOLECULAR TARGETED THERAPY; NEOPLASM PROTEINS; NITRILES; PAXILLIN; PHOSPHORYLATION; PROTO-ONCOGENE PROTEINS C-AKT; RADIATION TOLERANCE; TUMOR STEM CELL ASSAY;

EID: 84863620267     PISSN: 03603016     EISSN: 1879355X     Source Type: Journal    
DOI: 10.1016/j.ijrobp.2012.01.065     Document Type: Article

References (20)

1. C.H. Chung, S. Wong, and K.K. Ang Strategic plans to promote head and neck cancer translational research within the radiation therapy oncology group: a report from the translational research program Int J Radiat Oncol Biol Phys 69 2007 S67 S78
2. M. Baumann, M. Krause, and E. Dikomey EGFR-targeted anti-cancer drugs in radiotherapy: preclinical evaluation of mechanisms Radiother Oncol 83 2007 238 248
3. S. Hehlgans, M. Haase, and N. Cordes Signalling via integrins: implications for cell survival and anticancer strategies Biochim Biophys Acta 1775 2007 163 180
4. C.C. Park, H.J. Zhang, and E.S. Yao Beta1 integrin inhibition dramatically enhances radiotherapy efficacy in human breast cancer xenografts Cancer Res 68 2008 4398 4405
5. C.H. Chung, Q. Zhang, and E.M. Hammond Integrating epidermal growth factor receptor assay with clinical parameters improves risk classification for relapse and survival in head-and-neck squamous cell carcinoma Int J Radiat Oncol Biol Phys 81 2011 331 338
6. I. Eke, and N. Cordes Radiobiology goes 3D: how ECM and cell morphology impact on cell survival after irradiation Radiother Oncol 99 2011 271 278
7. I. Eke, U. Koch, and S. Hehlgans PINCH1 regulates Akt1 activation and enhances radioresistance by inhibiting PP1alpha J Clin Invest 120 2010 2516 2527
8. D.J. Sieg, C.R. Hauck, and D. Ilic FAK integrates growth-factor and integrin signals to promote cell migration Nat Cell Biol 2 2000 249 256
9. S. Hehlgans, I. Lange, and I. Eke 3D cell cultures of human head and neck squamous cell carcinoma cells are radiosensitized by the focal adhesion kinase inhibitor TAE226 Radiother Oncol 92 2009 371 378
10. J. Schwock, N. Dhani, and D.W. Hedley Targeting focal adhesion kinase signaling in tumor growth and metastasis Expert Opin Ther Targets 14 2010 77 94
11. N. Cordes, S. Frick, and T.B. Brunner Human pancreatic tumor cells are sensitized to ionizing radiation by knockdown of caveolin-1 Oncogene 26 2007 6851 6862
12. I. Eke, and N. Cordes Dual targeting of EGFR and focal adhesion kinase in 3D grown HNSCC cell cultures Radiother Oncol 99 2011 279 286
13. A.K. Nalla, S. Asuthkar, and P. Bhoopathi Suppression of uPAR retards radiation-induced invasion and migration mediated by integrin beta1/FAK signaling in medulloblastoma PLoS One 5 2010 e13006
14. I. Eke, Y. Deuse, and S. Hehlgans β1 Integrin/FAK/cortactin signaling is essential for human head and neck cancer resistance to radiotherapy J Clin Invest 122 2012 1529 1540
15. Q. Shi, A.B. Hjelmeland, and S.T. Keir A novel low-molecular weight inhibitor of focal adhesion kinase, TAE226, inhibits glioma growth Mol Carcin 46 2007 488 496
16. N. Watanabe, M. Takaoka, and K. Sakurama Dual tyrosine kinase inhibitor for focal adhesion kinase and insulin-like growth factor-I receptor exhibits anticancer effect in esophageal adenocarcinoma in vitro and in vivo Clin Cancer Res 14 2008 4631 4639
17. I.A. Kim, S.S. Bae, and A. Fernandes Selective inhibition of Ras, phosphoinositide 3 kinase, and Akt isoforms increases the radiosensitivity of human carcinoma cell lines Cancer Res 65 2005 7902 7910
18. D. Sano, F. Matsumoto, and D.R. Valdecanas Vandetanib restores head and neck squamous cell carcinoma cells' sensitivity to cisplatin and radiation in vivo and in vitro Clin Cancer Res 17 2011 1815 1827
19. A.K. Gupta, J.H. Lee, and W.W. Wilke Radiation response in two HPV-infected head-and-neck cancer cell lines in comparison to a non-HPV-infected cell line and relationship to signaling through AKT Int J Radiat Oncol Biol Phys 74 2009 928 933
20. E.J. Chung, A.P. Brown, and H. Asano In vitro and in vivo radiosensitization with AZD6244 (ARRY-142886), an inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase 1/2 kinase Clin Cancer Res 15 2009 3050 3057