Purification and characterization of chymosin and pepsin from kid

Journal of Dairy Research

Volumn 73, Issue 1, 2006, Pages 49-57

  Moschopoulou, Ekaterini E ^a   Kandarakis, Ioannis G ^a   Alichanidis, Efstathios ^b   Anifantakis, Emmanouil M ^a  

^a AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

^b ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Kid chymosin; Kid pepsin; Milk clotting enzymes

Indexed keywords

CHYMOSIN; PEPSIN A;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; COMPARATIVE STUDY; ENZYMOLOGY; FEMALE; GOAT; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; METABOLISM; MILK; MOLECULAR WEIGHT; PH; RUMINANT STOMACH; TEMPERATURE;

ABOMASUM; ANIMALS; CATTLE; CHYMOSIN; FEMALE; GOATS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; MILK; MOLECULAR WEIGHT; PEPSIN A; TEMPERATURE;

EID: 31544459568     PISSN: 00220299     EISSN: 14697629     Source Type: Journal    
DOI: 10.1017/S0022029905001470     Document Type: Article

References (39)

1. Alais C, Dutheil H & Bosc J 1962 [Specificity of rennet extracts from lamb's and calf's stomach for ewe's and cow's casein]. Proceedings of XVI International Dairy Congress, Kobenhann, 643-653
2. Amourache L & Vijayalakshmi MA 1984 Affinity chromatography of kid chymosin on histidyl-Sepharose. Journal of Chromatography 303 285-290
3. Andren A 1992 Production of prochymosin, pepsinogen and progastricsin and their cellular and intracellular localization in bovine abomasal mucosa. Scandinavia Journal of Clinical and Laboratory Investigations 54 (Suppl. 210) 59-64
4. Andrews AT 1983 Proteinases in normal bovine milk and their action on caseins. Journal of Dairy Research 50 45-55
5. Barzaghi S & Rampilli M 1996 [Evaluation of the enzymatic characteristics of commercial rennet pastes] Scienza e Tecnica Lattiero Casearia 47 171-181
6. Bines VE, Young P & Law BA 1989 Comparison of Cheddar cheese made with a recombinant calf chymosin and with standard calf rennet. Journal of Dairy Research 56 657-664
7. Baudys M, Erdene TG, Kostka V, Pavlik M & Foltmann B 1988 Comparison between prochymosin and pepsinogen from lamb and calf. Comparative Biochemistry and Physiology 89B 385-391
8. Chow RB & Kassell B 1968 Bovine pepsinogen and pepsin. I. Isolation, purification, and some properties of the pepsinogen. Journal of Biological Chemistry 243 1718-1724
9. Commission Regulation (EC) No 1081/96 Establishing a reference method for the detection of cows' milk and caseinate in cheeses made from ewes' milk, goats' milk or buffalos' milk or mixtures of ewes', goats' and buffalos' milk and repealing Regulation (EEC) No 690/92. Official Journal of the European Communities No L 142 15
10. Corradini C, Panari G, Rampilli M & Barzaghi S 1993 [Evaluation of the official method of analysis to identify the fermentation-produced chymosins]. Scienza e Tecnica Lattiero Casearia 44 195-204
11. Donnelly WJ, Carroll DP, O'Callaghan DM & Walls D 1986 Genetic polymorphism of bovine chymosin. Journal of Dairy Research 53 657-664
12. Foltmann B 1970 Prochymosin and chymosin (prorennin and rennin). In Methods in Enzymology, Vol. XIX proteolytic enzymes, pp. 421-436 (Eds GE Perlmann & L Lorand). New York: Academic press
13. Foltmann B, Pederson VB, Jacobsen M, Kauffman D & Wybrandt G 1977 Complete amino acid sequence of prochymosin. Proceedings National Academy of Science USA 74 2321-2324
14. Foltmann B, Pederson VB, Kauffman D & Wybrand G 1979 The primary structure of calf chymosin. Journal of Biological Chemistry 254 8447-8456
15. Fox PF 1969 Milk-clotting and proteolytic activities of rennet, and of bovine pepsin and porcine pepsin. Journal of Dairy Research 36 427-433
16. Fox PF, Whitaker JR & O'Leary PA 1977 Isolation and characterization of sheep pepsin. Biochemical Journal 161 389-398
17. Guinee TP & Wilkinson MG 1992 Rennet coagulation and coagulants in cheese manufacture. Journal of Society of Dairy Technology (now International Journal of Dairy Technology) 45(4) 94-103
18. International Dairy Federation 1990 Milk. Determination of total phosphorus content. Spectrometric method. Standard 42B Brussels: International Dairy Federation
19. International Dairy Federation 1997a Calf rennet and adult bovine rennet: Determination of Chymosin and Bovine Pepsin Contents (Chromatographic method). Standard 110B Brussels: International Dairy Federation
20. International Dairy Federation 1997b Bovine rennets: Determination of Total milk-clotting activity. Standard 157A Brussels: International Dairy Federation
21. Jordan MJ, Hellin P, Castillo M, Laencina J & Lopez MB 1999 Characterization and comparative study of animal rennets from different ruminants applied to goat milk. Milchwissenschaft 54 144-146
22. Kleinert TH, Lange I, Rosicke B, Honig A & Schleusener R 1988 Characterization and preparation of chymosin from calf rennet samples by means of isoelectric focusing. Acta Biotechnologica 8 367-375
23. Laemmli UK 1970 Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature 227 680-685
24. Lowry OH, Rosebrough NJ, Farrand AL & Randall RJ 1951 Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193 265-275
25. Martin P 1984 Influence du degre de phosphorylation de la pepsine A bovine sur son activite enzymatique. Biochimie 66 371-384
26. Martin P & Corre C 1984 Fractionation of the multiple forms of bovine gastric aspartic proteases by chromatofocusing. Analytical Biochemistry 143 256-264
27. Martin P, Trieu-Cuot P, Collin JC & Ribadeau-Dumas B 1982 Purification and characterization of bovine gastricsin. European Journal of Biochemistry 122 31-39
28. Meitner P & Kassell B 1971 Bovine pepsinogens and pepsins. Biochemical Journal 121 249-256
29. Mohanty AK, Mukhopadhyay UK, Kaushik JK, Grover S & Batish VK 2003 Isolation, purification and characterization of chymosin from riverine buffalo (Bufalos bubalis). Journal of Dairy Research 70 37-43
30. O'Leary PA & Fox PF 1973 Ovine pepsin: Suitability as a rennet substitute. Irish Journal of Agricultural Research 12 267-273
31. Panari G, Molinari P, Corradini C & Rampilli M 1990 [Chromatographic characterization by HPLC of enzymes of rennet and milk coagulants]. Scieza e Tecnica Lattiero Casearia 41 437-444
32. Pungercar J, Strukelj B, Gubensek F, Turk V & Kregar I 1990 Complete primary structure of lamb preprochymosin deduced from cDNA. Nucleic Acids Research 18 4602
33. Rampilli M, Barzaghi S, Molinari P & Tenaglia L 1992 HPLC analysis of rennet enzymes and fermentation-produced chymosin. Scinza e Tecnica Lattiero Casearia 43 387-401
34. Righetti PG, Molinari BM & Molinari G 1977 Isoelectric focusing of milk-clotting enzymes. Journal of Dairy Research 44 69-72
35. Rogelj I, Perko B, Francky A, Penca V & Pungercar J 2001 Recombinant lamb chymosin as an alternative coagulation enzyme in cheese production. Journal of Dairy Science 84 1020-1026
36. Shakeel-Ur-Rehman, Feeney EP, McSweeney PLH & Fox PF 1998 Inhibition of residual coagulant in cheese using pepstatin. International Dairy Journal 8 987-992
37. Suzuki M, Narita Y, Oda S, Moriyama A, Takenaka O & Kageyama T 1999 Purification and characterization of goat pepsinogens and pepsins. Comparative Biochemistry and Physiology Part B 122 453-460
38. Uchiyama H, Uozumi T, Beppu T & Arima K 1981 Purification of prorennin and production of its antibody. Journal of Biochemistry 90 483-487
39. Zollner H 1993 Handbook of enzyme inhibitors. 2nd ed, Part A. pp. 123, 217, 362, 898. New York: VCH