Development of a Multiplex PCR Technique for Detection and Epidemiological Typing of Salmonella in Human Clinical Samples

Journal of Clinical Microbiology

Volumn 42, Issue 4, 2004, Pages 1734-1738

  Alvarez, Juan ^a   Sota, Mertxe ^b   Vivanco, Ana Belén ^a   Perales, Ildefonso ^c   Cisterna, Ramón ^a,b   Rementeria, Aitor ^a   Garaizar, Javier ^a  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

^b HOSPITAL DE BASURTO   (Spain)

^c Public Health Laboratory   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIOPHAGE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; EPIDEMIOLOGICAL DATA; GENE AMPLIFICATION; HUMAN; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERITIDIS; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; SEROTYPE; SPAIN; VALIDATION PROCESS;

BACTERIAL TYPING TECHNIQUES; BACTERIOPHAGE TYPING; CULTURE MEDIA; DNA PRIMERS; DNA, BACTERIAL; FECES; HUMANS; POLYMERASE CHAIN REACTION; SALMONELLA; SALMONELLA ENTERITIDIS; SALMONELLA INFECTIONS; SALMONELLA PHAGES; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; SEROTYPING;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERITIDIS; SALMONELLA TYPHIMURIUM;

EID: 12244271953     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.4.1734-1738.2004     Document Type: Article

References (19)

1. Agron, P. G., R. L. Walker, H. Kinde, S. J. Sawyer, D. C. Hayes, J. Wollard, and G. L. Andersen. 2001. Identification by subtractive hybridization of sequences specific for Salmonella enterica serotype Enteritidis. Appl. Environ. Microbiol. 67:4984-4991.
2. Al-Soud, W. A., and P. Radstrom. 2000. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J. Clin. Microbiol. 38:4463-4470.
3. Garaizar, J., S. Porwollik, A. Echeita, A. Rementeria, S. Herrera, R. M. Wong, J. Frye, M. A. Usera, and M. McClelland. 2002. DNA microarray-based typing of an atypical monophasic Salmonella enterica serotype. J. Clin. Microbiol. 40:2074-2078.
4. Herikstad, H., Y. Motarjemi, and R. V. Tauxe. 2002. Salmonella surveillance: a global survey of public health serotyping. Epidemiol. Infect. 129:1-8.
5. Hong, Y., M. E. Berrang, T. Liu, C. L. Hofacre, S. Sanchez, L. Wang, and J. J. Maurer. 2003. Rapid detection of Campylobacter coli, C. jejuni, and Salmonella enterica on poultry carcasses by using PCR-enzyme-linked immunosorbent assay. Appl. Environ. Microbiol. 69:3492-3499.
6. Hoorfar, J., P. Ahrens, and P. Ro̊sdström. 2000. Automated 5′ nuclease assay for identification of Salmonella enterica. J. Clin. Microbiol. 38:3429-3435.
7. Kongmuang, U., J. M. C. Luk, and A. A. Lindberg. 1994. Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR. J. Clin. Microbiol. 32:3072-3074.
8. Kwang, J., E. T. Littledike, and J. E. Keen. 1996. Use of the polymerase chain reaction for Salmonella detection. Lett. Appl. Microbiol. 22:46-51.
9. Laconcha, I. 2001. Evaluación de marcadores moleculares en la tipificación de Salmonella spp. Creación de un sistema de librerías informatizado para la vigilancia epidemiológica. Doctoral thesis. University of the Basque Country, Vitoria-Gasteiz, Spain.
10. Lee, C. Y., G. Panicker, and A. K. Bej. 2003. Detection of pathogenic bacteria in shellfish using multiplex PCR followed by CovaLink NH microwell plate sandwich hybridization. J. Microbiol. Methods 53:199-209.
11. Lin, J. S., and H. Y. Tsen. 1999. Development and use of polymerase chain reaction for the detection of Salmonella Typhimurium in stool and food samples. J. Food Prot. 62:1103-1110.
12. López-Molina, N., I. Laconcha, A. Rementeria, A. Audicana, I. Perales, and J. Garaizar. 1998. Typing of Salmonella enteritidis of different phage types of PCR fingerprinting. J. Appl. Microbiol. 84:877-882.
13. Luk, J. M., U. Kongmuang, P. R. Reeves, and A. A. Lindberg. 1993. Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D). J. Clin. Microbiol. 31:2118-2123.
14. Malorny, B., J. Hoorfar, C. Bunge, and R. Helmuth. 2003. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl. Environ. Microbiol. 69:290-296.
15. Mason, W. J., J. S. Blevins, K. Beenken, N. Wibowo, N. Ojha, and M. S. Smeltzer. 2001. Multiplex PCR protocol for the diagnosis of staphylococcal infection. J. Clin. Microbiol. 39:3332-3338.
16. Olsen, J. E., S. Aabo, O. F. Rasmussen, and L. Rossen. 1995. Oligonucleotide probes specific for the genus Salmonella and for Salm. typhimurium. Lett. Appl. Microbiol. 20:160-163.
17. Pritchett, L. C., M. E. Konkel, J. M. Gay, and T. E. Besser. 2000. Identification of DT104 and U302 phage types among Salmonella enterica serotype Typhimurium isolates by PCR. J. Clin. Microbiol. 38:3484-3488.
18. Usera, M. A., A. Aladueña, R. Díaz, M. de la Fuente, R. Gutiérrez, P. Cerdán, M. Arroyo, R. González, and A. Echeita. 2001. Análisis de las cepas de Salmonella spp. aisladas de muestras clínicas de origen humano en España en el año 2000 (I). Bol. Epidemiol. Sem. 9:221-224.
19. Wang, G., C. G. Clark, and F. G. Rodgers. 2002. Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR. J. Clin. Microbiol. 40:3613-3619.