The analysis of major impurities of lipophilic-conjugated phosphorothioate oligonucleotides by ion-pair reversed-phase HPLC combined with MALDI-TOF-MS

Analytical and Bioanalytical Chemistry

Volumn 403, Issue 5, 2012, Pages 1333-1342

  Liu, Cai Hong ^a,b   Lu, Dan Dan ^b   Deng, Xin Xiu ^b   Wang, Ying ^b   Zhang, Jing Yu ^b   Zhang, Yu Lin ^b   Wang, Sheng Qi ^b,c  

^a MEDICAL SCHOOL OF CHINESE PLA   (China)

^b BEIJING INSTITUTE OF RADIATION MEDICINE   (China)

^c HENAN UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Analysis of major impurities; Ion pair reversed phase HPLC; Lipophilic conjugated phosphorothioate oligonucleotides; MALDL TOF MS

Indexed keywords

ANALYTICAL CONDITIONS; BUFFER COMPOSITION; CAPILLARY GEL ELECTROPHORESIS; CHROMATOGRAPHIC RETENTION; ION PAIRS; ION-PAIRING; MALDI TOF MS; MALDL-TOF-MS; MATRIX-ASSISTED LASER DESORPTION-IONIZATION TIME-OF-FLIGHT MASS; PHOSPHOROTHIOATE OLIGONUCLEOTIDE; PROCESS-RELATED IMPURITIES; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REVERSED PHASE HPLC; SEPARATION PERFORMANCE; SYNTHETIC PROCESS;

CAPILLARY ELECTROPHORESIS; CHROMATOGRAPHY; INDUCTIVELY COUPLED PLASMA; IONS; MASS SPECTROMETRY; OLIGONUCLEOTIDES; SEPARATION;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

OLIGONUCLEOTIDE PHOSPHOROTHIOATE;

ARTICLE; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; REVERSED PHASE LIQUID CHROMATOGRAPHY; SYNTHESIS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84862818431     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-5935-5     Document Type: Article

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