Separation of synthetic oligonucleotide dithioates from monothiophosphate impurities by anion-exchange chromatography on a Mono-Q column

Analytical Biochemistry

Volumn 306, Issue 1, 2002, Pages 92-99

  Yang, Xianbin ^a   Hodge, Richard P ^a   Luxon, Bruce A ^a   Shope, Robert ^b   Gorenstein, David G ^a  

^a Department of Human Biological Chemistry and Genetics ^*  (United States)

^b University of Texas Medical Branch School of Medicine   (United States)

Author keywords

Anion exchange chromatography; Mono Q column; Oligonucleotide phosphorodithioate; Oligonucleotide phosphoromonothioate

Indexed keywords

CHLORINE COMPOUNDS; COLUMN CHROMATOGRAPHY; IONS; LIQUID CHROMATOGRAPHY; SEPARATION; SYNTHESIS (CHEMICAL);

ANION EXCHANGE CHROMATOGRAPHY; HIGH RESOLUTION ANALYSIS; HIGH-RESOLUTION SEPARATIONS; SALT CONCENTRATION; SEQUENCE LENGTHS; SOLID-PHASE; STRONG ANION EXCHANGES;

OLIGONUCLEOTIDES;

OLIGONUCLEOTIDE; PHOSPHORODITHIOIC ACID DERIVATIVE; PHOSPHOROTHIOIC ACID; SODIUM CHLORIDE; THIOCYANATE; ANION EXCHANGE RESIN; MONO Q; OLIGODEOXYRIBONUCLEOTIDE; PHOSPHATE; PHOSPHORUS DERIVATIVE; RESIN; SULFUR DERIVATIVE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; ELUTION; MASS SPECTROMETRY; PH; PHOSPHORUS NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PURIFICATION; SOLID; CHEMISTRY; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEOTIDE SEQUENCE; TIME;

ANION EXCHANGE RESINS; BASE SEQUENCE; CHROMATOGRAPHY, ION EXCHANGE; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; OLIGODEOXYRIBONUCLEOTIDES; PHOSPHATES; PHOSPHORUS COMPOUNDS; RESINS, SYNTHETIC; SULFUR COMPOUNDS; TIME FACTORS;

EID: 0036629203     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5694     Document Type: Article

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