Reducing agents affect inhibitory activities of compounds: Results from multiple drug targets

Analytical Biochemistry

Volumn 423, Issue 1, 2012, Pages 46-53

  Lee, Hyun ^a   Torres, Jaime ^a   Truong, Lena ^a   Chaudhuri, Rima ^a   Mittal, Anuradha ^a   Johnson, Michael E ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

False negatives; False positives; Inhibitor screening; Potency comparison; Reducing agent

Indexed keywords

PHOSPHORUS COMPOUNDS; PROTEINS;

EXPERIMENTAL TESTING; FALSE NEGATIVES; FALSE POSITIVE; HIGH-THROUGHPUT SCREENING; INHIBITOR SCREENINGS; LARGE COMPOUND LIBRARIES; POTENCY COMPARISON; TRIS(2-CARBOXYETHYL) PHOSPHINE;

REDUCING AGENTS;

DITHIOTHREITOL; GLUTATHIONE; MERCAPTOETHANOL; REDUCING AGENT; TRIS(2 CARBOXYETHYL)PHOSPHINE;

ARTICLE; DRUG POTENCY; DRUG TARGETING; FEASIBILITY STUDY; HIGH THROUGHPUT SCREENING; IC 50; IN VITRO STUDY; PRIORITY JOURNAL;

EID: 84862804741     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.01.006     Document Type: Article

References (28)

1. B.Y. Feng, B.K. Shoichet, A detergent-based assay for the detection of promiscuous inhibitors, Nat. Protoc. 1 (2006) 550-553.
2. P.A. Johnston, C.A. Foster, T.Y. Shun, J.J. Skoko, S. Shinde, P. Wipf, J.S. Lazo, Development and implementation of a 384-well homogeneous fluorescence intensity high-throughput screening assay to identify mitogen-activated protein kinase phosphatase-1 dual-specificity protein phosphatase inhibitors, Assay Drug Dev. Technol. 5 (2007) 319-332. (Pubitemid 47067878)
3. M.B. Tierno, P.A. Johnston, C. Foster, J.J. Skoko, S.N. Shinde, T.Y. Shun, J.S. Lazo, Development and optimization of high-throughput in vitro protein phosphatase screening assays, Nat. Protoc. 2 (2007) 1134-1144. (Pubitemid 46901292)
4. L.A. Lor, J. Schneck, D.E. McNulty, E. Diaz, M. Brandt, S.H. Thrall, B. Schwartz, A simple assay for detection of small-molecule redox activity, J. Biomol. Screen. 12 (2007) 881-890. (Pubitemid 47340702)
5. M. Brisson, T. Nguyen, P. Wipf, B. Joo, B.W. Day, J.S. Skoko, E.M. Schreiber, C. Foster, P. Bansal, J.S. Lazo, Redox regulation of Cdc25B by cell-active quinolinediones, Mol. Pharmacol. 68 (2005) 1810-1820. (Pubitemid 41656028)
6. P.A. Johnston, K.M. Soares, S.N. Shinde, C.A. Foster, T.Y. Shun, H.K. Takyi, P. Wipf, J.S. Lazo, Development of a 384-well colorimetric assay to quantify hydrogen peroxide generated by the redox cycling of compounds in the presence of reducing agents, Assay Drug Dev. Technol. 6 (2008) 505-518.
7. G.K. Smith, D.G. Barrett, K. Blackburn, M. Cory, W.S. Dallas, R. Davis, D. Hassler, R. McConnell, M. Moyer, K. Weaver, Expression, preparation, and high-throughput screening of caspase-8: discovery of redox-based and steroid diacid inhibition, Arch. Biochem. Biophys. 399 (2002) 195-205. (Pubitemid 34848607)
8. A. Crowe, C. Ballatore, E. Hyde, J.Q. Trojanowski, V.M. Lee, High throughput screening for small molecule inhibitors of heparin-induced tau fibril formation, Biochem. Biophys. Res. Commun. 358 (2007) 1-6. (Pubitemid 46719073)
9. M.P. Bova, M.N. Mattson, S. Vasile, D. Tam, L. Holsinger, M. Bremer, T. Hui, G. McMahon, A. Rice, J.M. Fukuto, The oxidative mechanism of action of orthoquinone inhibitors of protein-tyrosine phosphatase alpha is mediated by hydrogen peroxide, Arch. Biochem. Biophys. 429 (2004) 30-41. (Pubitemid 39013380)
10. S. Keinan, W.D. Paquette, J.J. Skoko, D.N. Beratan, W. Yang, S. Shinde, P.A. Johnston, J.S. Lazo, P. Wipf, Computational design, synthesis, and biological evaluation of para-quinone-based inhibitors for redox regulation of the dual-specificity phosphatase Cdc25B, Org. Biomol. Chem. 6 (2008) 3256-3263.
11. K.M. Soares, N. Blackmon, T.Y. Shun, S.N. Shinde, H.K. Takyi, P. Wipf, J.S. Lazo, P.A. Johnston, Profiling the NIH small molecule repository for compounds that generate H2O2 by redox cycling in reducing environments, Assay Drug Dev. Technol. 8 (2010) 152-174.
12. I. Okun, S. Malarchuk, E. Dubrovskaya, A. Khvat, S. Tkachenko, V. Kysil, D. Kravchenko, A. Ivachtchenko, Screening for caspase-3 inhibitors: effect of a reducing agent on identified hit chemotypes, J. Biomol. Screen. 11 (2006) 694-703. (Pubitemid 44315967)
13. P. Simmonds, J. Bukh, C. Combet, G. Deleage, N. Enomoto, S. Feinstone, P. Halfon, G. Inchauspe, C. Kuiken, G. Maertens, M. Mizokami, D.G. Murphy, H. Okamoto, J.M. Pawlotsky, F. Penin, E. Sablon, I.T. Shin, L.J. Stuyver, H.J. Thiel, S. Viazov, A.J. Weiner, A. Widell, Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes, Hepatology 42 (2005) 962-973. (Pubitemid 41401136)
14. J. Dubuisson, F. Helle, L. Cocquerel, Early steps of the hepatitis C virus life cycle, Cell Microbiol. 10 (2008) 821-827. (Pubitemid 351386938)
15. M.J. Evans, T. von Hahn, D.M. Tscherne, A.J. Syder, M. Panis, B. Wolk, T. Hatziioannou, J.A. McKeating, P.D. Bieniasz, C.M. Rice, Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry, Nature 446 (2007) 801-805. (Pubitemid 46582027)
16. A. Zheng, F. Yuan, Y. Li, F. Zhu, P. Hou, J. Li, X. Song, M. Ding, H. Deng, Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus, J. Virol. 81 (2007) 12465-12471.
17. V. Rocha-Perugini, C. Montpellier, D. Delgrange, C. Wychowski, F. Helle, A. Pillez, H. Drobecq, F. Le Naour, S. Charrin, S. Levy, E. Rubinstein, J. Dubuisson, L. Cocquerel, The CD81 partner EWI-2wint inhibits hepatitis C virus entry, PLoS One 3 (2008) e1866.
18. H. Yang, M. Bartlam, Z. Rao, Drug design targeting the main protease, the Achilles' heel of coronaviruses, Curr. Pharm. Des. 12 (2006) 4573-4590. (Pubitemid 44835146)
19. J. Ziebuhr, The coronavirus replicase, Curr. Top. Microbiol. Immunol. 287 (2005) 57-94. (Pubitemid 39371915)
20. L.B. Seeff, Natural history of chronic hepatitis C, Hepatology 36 (2002) S35-S46. (Pubitemid 35253462)
21. J.S. Peiris, S.T. Lai, L.L. Poon, Y. Guan, L.Y. Yam, W. Lim, J. Nicholls, W.K. Yee, W.W. Yan, M.T. Cheung, V.C. Cheng, K.H. Chan, D.N. Tsang, R.W. Yung, T.K. Ng, K.Y. Yuen, Coronavirus as a possible cause of severe acute respiratory syndrome, Lancet 361 (2003) 1319-1325. (Pubitemid 36514862)
22. T.G. Ksiazek, D. Erdman, C.S. Goldsmith, S.R. Zaki, T. Peret, S. Emery, S. Tong, C. Urbani, J.A. Comer, W. Lim, P.E. Rollin, S.F. Dowell, A.E. Ling, C.D. Humphrey, W.J. Shieh, J. Guarner, C.D. Paddock, P. Rota, B. Fields, J. DeRisi, J.Y. Yang, N. Cox, J.M. Hughes, J.W. LeDuc, W.J. Bellini, L.J. Anderson, A novel coronavirus associated with severe acute respiratory syndrome, N. Engl. J. Med. 348 (2003) 1953-1966. (Pubitemid 36569984)
23. C. Drosten, S. Gunther, W. Preiser, S. van der Werf, H.R. Brodt, S. Becker, H. Rabenau, M. Panning, L. Kolesnikova, R.A. Fouchier, A. Berger, A.M. Burguiere, J. Cinatl, M. Eickmann, N. Escriou, K. Grywna, S. Kramme, J.C. Manuguerra, S. Muller, V. Rickerts, M. Sturmer, S. Vieth, H.D. Klenk, A.D. Osterhaus, H. Schmitz, H.W. Doerr, Identification of a novel coronavirus in patients with severe acute respiratory syndrome, N. Engl. J. Med. 348 (2003) 1967-1976. (Pubitemid 36569985)
24. J.J. Irwin, B.K. Shoichet, ZINC-a free database of commercially available compounds for virtual screening, J. Chem. Inf. Model. 45 (2005) 177-182. (Pubitemid 40736970)
25. R. Macarron, R.P. Hertzberg, Design and implementation of high throughput screening assays, Mol. Biotechnol. 47 (2011) 270-285.
26. D. Lee, S.A. Long, J.L. Adams, G. Chan, K.S. Vaidya, T.A. Francis, K. Kikly, J.D. Winkler, C.M. Sung, C. Debouck, S. Richardson, M.A. Levy, W.E. DeWolf Jr., P.M. Keller, T. Tomaszek, M.S. Head, M.D. Ryan, R.C. Haltiwanger, P.H. Liang, C.A. Janson, P.J. McDevitt, K. Johanson, N.O. Concha, W. Chan, S.S. Abdel-Meguid, A.M. Badger, M.W. Lark, D.P. Nadeau, L.J. Suva, M. Gowen, M.E. Nuttall, Potent and selective nonpeptide inhibitors of caspases 3 and 7 inhibit apoptosis and maintain cell functionality, J. Biol. Chem. 275 (2000) 16007-16014. (Pubitemid 30366906)
27. D. Lee, S.A. Long, J.H. Murray, J.L. Adams, M.E. Nuttall, D.P. Nadeau, K. Kikly, J.D. Winkler, C.M. Sung, M.D. Ryan, M.A. Levy, P.M. Keller, W.E. DeWolf Jr., Potent and selective nonpeptide inhibitors of caspases 3 and 7, J. Med. Chem. 44 (2001) 2015-2026. (Pubitemid 32884524)
28. M. Tsiang, G.S. Jones, M. Hung, D. Samuel, N. Novikov, S. Mukund, K.M. Brendza, A. Niedziela-Majka, D. Jin, X. Liu, M. Mitchell, R. Sakowicz, R. Geleziunas, Dithiothreitol causes HIV-1 integrase dimer dissociation while agents interacting with the integrase dimer interface promote dimer formation, Biochemistry 50 (2011) 1567-1581.