Differential in-gel electrophoresis (DIGE) analysis of CHO cells under hyperosmotic pressure: Osmoprotective effect of glycine betaine addition

Biotechnology and Bioengineering

Volumn 109, Issue 6, 2012, Pages 1395-1403

  Kim, Jee Yon ^a   Kim, Yeon Gu ^b   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

DIGE; Glycine betaine; Hyperosmolality; Proteomics; RCHO cells

Indexed keywords

2D DIGE; ANTIBODY PRODUCTION; CELLULAR ARCHITECTURE; CELLULAR METABOLISM; CHINESE HAMSTER OVARY; CHO CELL; DIGE; GLYCINE BETAINE; HYPEROSMOLALITY; HYPEROSMOTIC CONDITIONS; MASS SPECTROMETRIC ANALYSIS; OSMOPROTECTIVE EFFECTS; PROTEIN PRODUCTION; PROTEIN SECRETION; PROTEIN SPOTS; PROTEOMICS; WESTERN-BLOT ANALYSIS;

AMINO ACIDS; CELLS; CYTOLOGY; ELECTROPHORESIS; MOLECULAR BIOLOGY; PROTEIN FOLDING; RECOMBINANT PROTEINS;

FACTOR ANALYSIS;

ACF PROTEIN; BETA ACTIN; BETA TUBULIN; BETAINE; CELL PROTEIN; GLUCOSE REGULATED PROTEIN 78; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INOSITOL POLYPHOSPHATE MULTIKINASE; MESSENGER RNA; OSMOTIC STRESS PROTEIN 94; PEPCK PROTEIN; PEROXIDOXIN 1; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); PHOSPHOTRANSFERASE; RNA BINDING MOTIF PROTEIN 34; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL ENGINEERING; CELL GROWTH; CELL METABOLISM; CELL PROTECTION; CHO CELL; CONTROLLED STUDY; DIFFERENTIAL IN GEL ELECTROPHORESIS; FEMALE; GEL ELECTROPHORESIS; HYPEROSMOTIC PRESSURE; MASS SPECTROMETRY; NONHUMAN; OSMOTIC PRESSURE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN SECRETION; RNA PROCESSING; WESTERN BLOTTING;

ANIMALS; BETAINE; BLOTTING, WESTERN; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITHELIAL CELLS; MASS SPECTROMETRY; OSMOTIC PRESSURE; PROTEOME; STRESS, PHYSIOLOGICAL;

CRICETULUS GRISEUS;

EID: 84862798518     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24442     Document Type: Article

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