Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines

Biotechnology and Bioengineering

Volumn 70, Issue 2, 2000, Pages 167-175

  Ryu, Joon Soo ^a   Kim, Tae Kyung ^a   Chung, Joo Young ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Foreign protein production; Glycine betaine; Hyperosmotic pressure; Recombinant Chinese hamster ovary (rCHO) cells; Specific productivity

Indexed keywords

ANIMAL CELL CULTURE; BATCH CELL CULTURE; COMPOSITION EFFECTS; OSMOSIS; PHYSIOLOGY; PRESSURE EFFECTS; RNA; SODIUM CHLORIDE;

GLYCINE BETAINE;

ANTIBODIES;

BETAINE; CELL PROTEIN;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL LINE; CELL PROTECTION; CHO CELL; GENE DOSAGE; HYPEROSMOLARITY; NONHUMAN; NORTHERN BLOTTING; OSMOSIS; PROTEIN SYNTHESIS;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0034693457     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20001020)70:2<167::AID-BIT6>3.0.CO;2-P     Document Type: Article

References (25)

1. Hyperosmolality leads to an increase in tissue-type plasminogen activator production by a Chinese hamster ovary cell line
2. Increased synthesis of secreted proteins induces expression of glucose-regulated proteins in butyrate-treated Chinese hamster ovary cells
3. Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate
4. Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture
5. Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for pre-S2 antigen of hepatitis B virus
6. Evolution of chromosomal regions containing transfected and amplified dihydrofolate reductase sequences
7. Decreased chimeric antibody productivity of KR12H-1 transfectoma during long-term culture results from decreased antibody gene copy number
8. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure
9. Extrinsic protein stabilization by the naturally occurring osmolytes beta-hydroxyectoine and betaine
10. Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific
11. Production of monoclonal antibody using free-suspended and immobilized hybridoma cells: Effects of serum
12. Enhanced monoclonal antibody production by gradual increase of osmotic pressure
13. Survey of osmolytes in renal cell lines
14. Osmoregulation of betaine transport in mammalian renal medullary cells
15. Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate
16. Intracellular responses of productive hybridomas subjected to high osmotic pressure
17. Utilization of osmoprotective compounds by hybridoma cells exposed to hyperosmotic stress
18. Hyperosmotic hybridoma cell cultures: Increased monoclonal antibody production with addition of glycine betaine
19. Transport of osmoprotective compounds in hybridoma cells exposed to hyperosmotic stress
20. Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production
21. Effects of abrupt and gradual osmotic stress on antibody production and content in hybridoma cells that differ in production kinetics
22. Influence of hyperosmolar basal media on hybridoma cell growth and antibody production
23. Manufacture of proteins based on recombinant Chinese hamster ovary cells: Assessment of genetic issues and assurance of consistency and quality
24. Living with water stress: Evolution of osmolyte systems