Use of NaCl prevents aggregation of recombinant COMP-Angiopoietin-1 in Chinese hamster ovary cells

Journal of Biotechnology

Volumn 143, Issue 2, 2009, Pages 145-150

  Ju, Hye Kyung ^a   Hwang, Su Jeong ^b   Jeon, Choon Ju ^a   Lee, Gyun Min ^b   Yoon, Sung Kwan ^a  

^a Aprogen   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Aggregation; CHO cells; COMP Ang1; Hyperosmotic medium; Specific COMP Ang1 productivity

Indexed keywords

AGGREGATION; CHO CELLS; COMP-ANG1; HYPEROSMOTIC MEDIUM; SPECIFIC COMP-ANG1 PRODUCTIVITY;

ALCOHOLS; BOTTLES; CELL CULTURE; CELL MEMBRANES; GROWTH KINETICS; PRODUCTIVITY; SODIUM ALLOYS; SODIUM CHLORIDE;

AGGLOMERATION;

ANGIOPOIETIN 1; CARTILAGE OLIGOMERIC MATRIX PROTEIN; SODIUM CHLORIDE; SORBITOL;

ANIMAL CELL; ARTICLE; CELL GROWTH; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; GENE EXPRESSION; HYPEROSMOLARITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ENGINEERING; PROTEIN SYNTHESIS;

ANIMALS; BLOTTING, WESTERN; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; OSMOLAR CONCENTRATION; POLYMERASE CHAIN REACTION; PROTEIN MULTIMERIZATION; RECOMBINANT FUSION PROTEINS; SODIUM CHLORIDE; SORBITOL;

CRICETULUS GRISEUS;

EID: 68549086867     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.06.017     Document Type: Article

References (19)

1. Brindle N.P.J., Saharinen P., and Alitalo K. Signaling and functions of Angiopoietin-1 in vascular protection. Circ. Res. 98 (2006) 1014-1023
2. Burg M.B., and Ferraris J.D. Intracellular organic osmolytes: function and regulation. J. Biol. Chem. 283 (2008) 7309-7313
3. Cho C.-.H., Kammerer R.A., Lee H.J., Steinmetz M.O., Ryu Y.S., Lee S.H., Yasunaga K., Kim K.-.T., Kim I., Choi H.-.H., Kim W., Kim S.H., Park S.K., Lee G.M., and Kho G.Y. COMP-Ang1: a designed Angiopoietin-1 variant with nonleaky angiogenic activity. Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 5547-5552
4. Cromwell M.E.M., Hilario E., and Jacobson F. Protein aggregation and bioprocessing. AAPS J. 8 (2006) E572-E579
5. Daugherty A.L., and Mrsny R.J. Formulation and delivery issues for monoclonal antibody therapeutics. Adv. Drug Delivery Rev. 58 (2006) 686-706
6. Davis S., Papadopoulos N., Aldrich T.H., Maisonpierre P.C., Huang T., Kovac L., Xu A., Leidich R., Radziejewska E., Rafique A., Goldberg J., Jain V., Bailey K., Karow M., Fandl J., Samuelsson S.J., Ioffe E., Rudge J.S., Daly T.J., Radziejewski C., and Yancopoulos G.D. Angiopoietins have distinct modular domains essential for receptor binding, dimerization and superclustering. Nat. Struct. Biol. 10 (2003) 38-44
7. Hwang S.J., Kim S.H., Kim H.Z., Steinmetz M.O., Koh G.Y., and Lee G.M. High-level expression and purification of a designed Angiopoietin-1 chimeric protein, COMP-Ang1, produced in Chinese hamster ovary cells. Protein J. 27 (2008) 319-326
8. Kempf B., and Bremer E. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch. Microbiol. 170 (1998) 319-330
9. Kim N.S., and Lee G.M. Response of recombinant Chinese hamster ovary cells to hyperosmotic pressure: effect of Bcl-2 overexpression. J. Biotechnol. 95 (2002) 237-248
10. Kim I., Moon S.O., Park S.K., Chae S.W., and Koh G.Y. Angiopoietin-1 reduces VEGF-stimulated leukocyte adhesion to endothelial cells by reducing ICAM-1, VCAM-1, and E-selectin expression. Circ. Res. 89 (2001) 477-479
11. Narhi L.O., Philo J.S., Sun B., Chang B.S., and Arakawa T. Reversibility of heat-induced denaturation of the recombinant human megakaryocyte growth and development factor. Pharm. Res. 16 (1999) 799-807
12. Procopio W.N., Pelavin P.I., Lee W.M., and Yeilding N.M. Angiopoietin-1 and -2 coiled coil domains mediate distinct homo-oligomerization patterns, but fibrinogen-like domains mediate ligand activity. J. Biol. Chem. 274 (1999) 30196-30201
13. Rodriguez J., Spearman M., Huzel N., and Butler M. Enhanced production of monomeric interferon-β by CHO cells through the control of culture conditions. Biotechnol. Prog. 21 (2005) 22-30
14. Ryff J.-C., and Schellekens H. Immunogenicity of rDNA-derived pharmaceuticals. Trends Pharmacol. Sci. 23 (2002) 254-256
15. Ryu J.S., Kim T.K., Chung J.Y., and Lee G.M. Osmoprotectant effect of glycine betaine on foreign protein production in hyperosmotic recombinant Chinese hamster ovary cell cultures differs among cell lines. Biotechnol. Bioeng. 70 (2000) 167-175
16. Ryu J.S., Lee M.S., and Lee G.M. Effects of cloned gene dosage on the response of recombinant CHO cells to hyperosmotic pressure in regard to cell growth and antibody production. Biotechnol. Prog. 17 (2001) 993-999
17. Spearman M., Rodriguez J., Huzel N., Sunley K., and Butler M. Effect of culture conditions on glycosylation of recombinant beta-interferon in CHO cells. In: Smith R. (Ed). Cell Technology for Cell Products (2007), Springer 71-85
18. Wang W. Protein aggregation and its inhibition in biopharmaceutics. Int. J. Pharm. 289 (2005) 1-30
19. Welch W.J., and Brown C.R. Influence of molecular and chemical chaperones on protein folding. Cell Stress Chaperon. 1 (1996) 109-115