Sulfonium ion derivatization, isobaric stable isotope labeling and data dependent CID- and ETD-MS/MS for enhanced phosphopeptide quantitation, identification and phosphorylation site characterization

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 4, 2012, Pages 577-593

  Lu, Yali ^a   Zhou, Xiao ^a   Stemmer, Paul M ^b   Reid, Gavin E ^a,c  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b WAYNE STATE UNIVERSITY   (United States)

^c Michigan State University   (United States)

Author keywords

Collision induced dissociation (CID); Electron transfer dissociation (ETD); Fixed charge; Phosphopeptide fragmentation

Indexed keywords

AUTOMATED DATA; CAPILLARY HPLC; CHARGE STATE; COLLISION INDUCED DISSOCIATION; DATA DEPENDENT; DERIVATIZATIONS; DETRIMENTAL EFFECTS; DIMETHYLSULFIDE; ELECTRON-TRANSFER DISSOCIATION; FIXED CHARGES; FRAGMENTATION EFFICIENCY; HPLC-ESI MS; ION TRAPS; IONIZATION EFFICIENCY; NEUTRAL LOSS; PHOSPHATE GROUP; PHOSPHOPEPTIDES; PHOSPHORYLATION SITES; PRECURSOR IONS; PROTEIN PHOSPHORYLATION; SEQUENCE IDENTIFICATION; STABLE ISOTOPES; STABLE-ISOTOPE LABELING;

CHARACTERIZATION; DISSOCIATION; ISOTOPES; ORGANIC COMPOUNDS; PEPTIDES; PHOSPHORYLATION;

IONS;

DIMETHYL SULFIDE; DIMETHYLTHIOBUTANOYLHYDROXYSUCCINIMIDE ESTER IODINE; PHOSPHOPEPTIDE; REAGENT; SULFONIUM DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; COLLISIONALLY ACTIVATED DISSOCIATION; DERIVATIZATION; ELECTRON TRANSFER DISSOCIATION; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIZATION; ISOTOPE LABELING; MASS SPECTROMETRY; PEPTIDE ANALYSIS; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; QUANTITATIVE ANALYSIS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; IONS; ISOTOPE LABELING; MOLECULAR SEQUENCE DATA; PHOSPHOPEPTIDES; PHOSPHORYLATION; SUCCINIMIDES; SULFONIUM COMPOUNDS; TANDEM MASS SPECTROMETRY;

EID: 84862516061     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0190-0     Document Type: Article

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