The influence of leukocytospermia on the outcomes of assisted reproductive technology

Reproductive Biology and Endocrinology

Volumn 10, Issue , 2012, Pages

  Cavagna, Mario ^a,b,c   Oliveira, Joao Batista A ^a,c,d   Petersen, Claudia G ^a,c,d   Mauri, Ana L ^a,c   Silva, Liliane F I ^a,c,d   Massaro, Fabiana C ^a,c   Baruffi, Ricardo L R ^a,c   Franco Jr, José G ^a,c,d  

^a Center for Human Reproduction Prof Franco Jr   (Brazil)

^b Hospital Perola Byington   (Brazil)

^c Research and Training   (Brazil)

^d SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

DNA damage; ICSI; IMSI; Leukocytospermia; Reactive oxygen species

Indexed keywords

GONADORELIN DERIVATIVE; RECOMBINANT FOLLITROPIN; RECOMBINANT LUTEINIZING HORMONE;

ARTICLE; CHI SQUARE TEST; CLINICAL ARTICLE; CONTROLLED STUDY; DATA ANALYSIS; FEMALE; HEMATOLOGIC DISEASE; HUMAN; HUMAN CELL; IMPLANTATION; INFERTILITY THERAPY; INTRACYTOPLASMIC SPERM INJECTION; LEUKOCYTE COUNT; LEUKOCYTOSPERMIA; LIVE BIRTH; MALE; MALE GENITAL SYSTEM DISEASE; MEDICAL RECORD; OUTCOME ASSESSMENT; RANK SUM TEST; SPONTANEOUS ABORTION; STUDENT T TEST;

ADULT; FEMALE; HUMANS; LEUKOCYTE COUNT; LEUKOCYTES; MALE; PREGNANCY; PREGNANCY OUTCOME; REPRODUCTIVE TECHNIQUES, ASSISTED; SEMEN; SPERM INJECTIONS, INTRACYTOPLASMIC; SPERMATOZOA; TREATMENT OUTCOME;

EID: 84862210649     PISSN: None     EISSN: 14777827     Source Type: Journal    
DOI: 10.1186/1477-7827-10-44     Document Type: Article

References (58)

1. World Health Organization WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. edn 1999, Published on behalf of the World Health Organization by Cambridge University Press, Cambridge, UK, World Health Organization.
2. Gambera L, Serafini F, Morgante G, Focarelli R, De Leo V, Piomboni P. Sperm quality and pregnancy rate after COX-2 inhibitor therapy of infertile males with abacterial leukocytospermia. Hum Reprod 2007, 22:1047-1051. 10.1093/humrep/del490, 17208944.
3. Kiessling AA, Lamparelli N, Yin HZ, Seibel MM, Eyre RC. Semen leukocytes: friends or foes?. Fertil Steril 1995, 64:196-198.
4. Fedder J. Nonsperm cells in human semen: with special reference to seminal leukocytes and their possible influence on fertility. Fertil Steril 1996, 36:41-65.
5. Arata de Bellabarba G, Tortolero I, Villarroel V, Molina CZ, Bellabarba C, Velazquez E. Nonsperm cells in human semen and their relationship with semen parameters. Fertil Steril 2000, 45:131-136.
6. Henkel R, Ichikawa T, Sanchez R, Miska W, Ohmori H, Schill WB. Differentiation of ejaculates showing reactive oxygen species production by spermatozoa or leukocytes. Andrologia 1997, 29:295-301.
7. Saleh RA, Agarwal A, Kandirali E, Sharma RK, Thomas AJ, Nada EA, Evenson DP, Alvarez JG. Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa. Fertil Steril 2002, 78:1215-1224. 10.1016/S0015-0282(02)04237-1, 12477515.
8. Lemkecher T, Dartigues S, Vaysse J, Kulski O, Barraud-Lange V, Gattegno L, Wolf JP. Leucocytospermia, oxidative stress and male fertility: facts and hypotheses. Gynecol Obstet Fertil 2005, 33:2-10. 10.1016/j.gyobfe.2005.01.001, 15752659.
9. Moskovtsev SI, Willis J, White J, Mullen JB. Leukocytospermia: relationship to sperm deoxyribonucleic acid integrity in patients evaluated for male factor infertility. Fertil Steril 2007, 88:737-740. 10.1016/j.fertnstert.2006.11.132, 17336968.
10. Fariello RM, Del Giudice PT, Spaine DM, Fraietta R, Bertolla RP, Cedenho AP. Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity. J Assist Reprod Genet 2009, 26:151-157. 10.1007/s10815-008-9288-0, 2654935, 19184399.
11. Domes T, Lo KC, Grober ED, Mullen JB, Mazzulli T, Jarvi K. The incidence and effect of bacteriospermia and elevated seminal leukocytes on semen parameters. Fertil Steril 2012, 97:1050-1055. 10.1016/j.fertnstert.2012.01.124, 22341372.
12. Talbert LM, Hammond MG, Halme J, O'Rand M, Fryer JG, Ekstrom RD. Semen parameters and fertilization of human oocytes in vitro: a multivariable analysis. Fertil Steril 1987, 48:270-277.
13. Krausz C, Mills C, Rogers S, Tan SL, Aitken RJ. Stimulation of oxidant generation by human sperm suspensions using phorbol esters and formyl peptides: relationships with motility and fertilization in vitro. Fertil Steril 1994, 62:599-605.
14. Sukcharoen N, Keith J, Irvine DS, Aitken RJ. Predicting the fertilizing potential of human sperm suspensions in vitro: importance of sperm morphology and leukocyte contamination. Fertil Steril 1995, 63:1293-1300.
15. Yilmaz S, Koyuturk M, Kilic G, Alpak O, Aytoz A. Effects of leucocytospermia on semen parameters and outcomes of intracytoplasmic sperm injection. Int J Androl 2005, 28:337-342. 10.1111/j.1365-2605.2005.00562.x, 16300665.
16. Lackner JE, Mark I, Sator K, Huber J, Sator M. Effect of leukocytospermia on fertilization and pregnancy rates of artificial reproductive technologies. Fertil Steril 2008, 90:869-871. 10.1016/j.fertnstert.2007.07.1310, 18001714.
17. Barraud-Lange V, Pont JC, Ziyyat A, Pocate K, Sifer C, Cedrin-Durnerin I, Fechtali B, Ducot B, Wolf JP. Seminal leukocytes are Good Samaritans for spermatozoa. Fertil Steril 2011, 96:1315-1319. 10.1016/j.fertnstert.2011.09.035, 22047665.
18. Petersen CG, Vagnini LD, Mauri AL, Massaro FC, Cavagna M, Baruffi RL, Oliveira JB, Franco JG. Relationship between DNA damage and sperm head birefringence. Reprod Biomed Online 2011, 22:583-589. 10.1016/j.rbmo.2011.03.017, 21507722.
19. Petersen CG, Massaro FC, Mauri AL, Oliveira JB, Baruffi RL, Franco JG. Efficacy of hyaluronic acid binding assay in selecting motile spermatozoa with normal morphology at high magnification. Reprod Biol Endocrinol 2010, 8:149. 10.1186/1477-7827-8-149, 3016298, 21129168.
20. Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y. Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl 2002, 23:1-8.
21. Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, Artzi S, Gross M, Barak Y. Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril 2003, 80:1413-1419. 10.1016/j.fertnstert.2003.05.016, 14667877.
22. Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Dd'Angelo D, Antinori S. Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online 2008, 16:835-841. 10.1016/S1472-6483(10)60150-2, 18549694.
23. Nadalini M, Tarozzi N, Distratis V, Scaravelli G, Borini A. Impact of intracytoplasmic morphologically selected sperm injection on assisted reproduction outcome: a review. Reprod Biomed Online 2009, 19(Suppl 3):45-55.
24. Oliveira JB, Massaro FC, Mauri AL, Petersen CG, Nicoletti AP, Baruffi RL, Franco JG. Motile sperm organelle morphology examination is stricter than Tygerberg criteria. Reprod Biomed Online 2009, 18:320-326. 10.1016/S1472-6483(10)60088-0, 19298729.
25. Oliveira JB, Massaro FC, Baruffi RL, Mauri AL, Petersen CG, Silva LF, Vagnini LD, Franco JG. Correlation between semen analysis by motile sperm organelle morphology examination and sperm DNA damage. Fertil Steril 2010, 94:1937-1940. 10.1016/j.fertnstert.2010.01.042, 20189558.
26. Wilding M, Coppola G, di Matteo L, Palagiano A, Fusco E, Dale B. Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet 2011, 28:253-262. 10.1007/s10815-010-9505-5, 3082654, 21072684.
27. Cassuto NG, Bouret D, Plouchart JM, Jellad S, Vanderzwalmen P, Balet R, Larue L, Barak Y. A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality. Fertil Steril 2009, 92:1616-1625. 10.1016/j.fertnstert.2008.08.088, 18990390.
28. Knez K, Zorn B, Tomazevic T, Vrtacnik-Bokal E, Virant-Klun I. The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: a comparative prospective randomized study. Reprod Biol Endocrinol 2011, 9:123. 10.1186/1477-7827-9-123, 3170257, 21875440.
29. Balaban B, Yakin K, Alatas C, Oktem O, Isiklar A, Urman B. Clinical outcome of intracytoplasmic injection of spermatozoa morphologically selected under high magnification: a prospective randomized study. Reprod Biomed Online 2011, 22:472-476.
30. Oliveira JB, Cavagna M, Petersen CG, Mauri AL, Massaro FC, Silva LF, Baruffi RL, Franco JG. Pregnancy outcomes in women with repeated implantation failures after intracytoplasmic morphologically selected sperm injection (IMSI). Reprod Biol Endocrinol 2011, 9:99. 10.1186/1477-7827-9-99, 3156729, 21781299.
31. Sermondade N, Hafhouf E, Dupont C, Bechoua S, Palacios C, Eustache F, Poncelet C, Benzacken B, Levy R, Sifer C. Successful childbirth after intracytoplasmic morphologically selected sperm injection without assisted oocyte activation in a patient with globozoospermia. Hum Reprod 2011, 26:2944-2949. 10.1093/humrep/der258, 21857011.
32. Cassuto NG, Hazout A, Benifla JL, Balet R, Larue L, Viot G. Decreasing birth defect in children by using high magnification selected spermatozoon injection. Fertil Steril 2011, 96:S85.
33. Aziz N, Agarwal A, Lewis-Jones I, Sharma RK, Thomas AJ. Novel associations between specific sperm morphological defects and leukocytospermia. Fertil Steril 2004, 82:621-627. 10.1016/j.fertnstert.2004.02.112, 15374705.
34. ASTM Standart E1951-02 Physical and mechanical testing standards 2007, West Conshohocken, PA, www.astm.org , ASTM.
35. Tomlinson MJ, White A, Barratt CL, Bolton AE, Cooke ID. The removal of morphologically abnormal sperm forms by phagocytes: a positive role for seminal leukocytes?. Hum Reprod 1992, 7:517-522.
36. Ziyyat A, Barraud-Lange V, Sifer C, Ducot B, Wolf JP, Soufir JC. Paradoxical increase of sperm motility and seminal carnitine associated with moderate leukocytospermia in infertile patients. Fertil Steril 2008, 90:2257-2263. 10.1016/j.fertnstert.2007.10.032, 18439589.
37. Aitken RJ, Baker HW. Seminal leukocytes: passengers, terrorists or good samaritans?. Hum Reprod 1995, 10:1736-1739.
38. Wolff H. The biologic significance of white blood cells in semen. Fertil Steril 1995, 63:1143-1157.
39. Sharma RK, Pasqualotto AE, Nelson DR, Thomas AJ, Agarwal A. Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic. J Androl 2001, 22:575-583.
40. Punab M, Loivukene K, Kermes K, Mandar R. The limit of leucocytospermia from the microbiological viewpoint. Andrologia 2003, 35:271-278.
41. Cooper TG, Hellenkemper B. Improving precision in the assessment of round cell numbers in human semen. Asian J Androl 2010, 12:111-114. 10.1038/aja.2009.15, 20111090.
42. Lackner JE, Lakovic E, Waldhor T, Schatzl G, Marberger M. Spontaneous variation of leukocytospermia in asymptomatic infertile males. Fertil Steril 2008, 90:1757-1760. 10.1016/j.fertnstert.2007.08.041, 18054920.
43. Tremellen K, Tunc O. Macrophage activity in semen is significantly correlated with sperm quality in infertile men. Int J Androl 2010, 33:823-831. 10.1111/j.1365-2605.2009.01037.x, 20132344.
44. Erenpreiss J, Hlevicka S, Zalkalns J, Erenpreisa J. Effect of leukocytospermia on sperm DNA integrity: a negative effect in abnormal semen samples. J Androl 2002, 23:717-723.
45. Ricci G, Perticarari S, Boscolo R, Simeone R, Martinelli M, Fischer-Tamaro L, Guaschino S, Presani G. Leukocytospermia and sperm preparation-a flow cytometric study. Reprod Biol Endocrinol 2009, 7:128. 10.1186/1477-7827-7-128, 2789083, 19925640.
46. Tremellen K. Oxidative stress and male infertility-a clinical perspective. Hum Reprod update 2008, 14:243-258. 10.1093/humupd/dmn004, 18281241.
47. Ford WC. Comments on the release of the 5th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen. Asian J Androl 2010, 12:59-63. 10.1038/aja.2008.57, 20111082.
48. Ramya T, Misro MM, Sinha D, Nandan D, Mithal S. Altered levels of seminal nitric oxide, nitric oxide synthase, and enzymatic antioxidants and their association with sperm function in infertile subjects. Fertil Steril 2011, 95:135-140. 10.1016/j.fertnstert.2010.06.044, 20684956.
49. Herrero MB, Gagnon C. Nitric oxide: a novel mediator of sperm function. J Androl 2001, 22:349-356.
50. Vicino M, Loverro G, Simonetti S, Mei L, Selvaggi L. The correlation between idiopathic leukocytospermia, embryo quality and outcome in the FIVET and ICSI procedures. Minerva Ginecol 1999, 51:413-420.
51. Barraud-Lange V, Pont JC, Pocate K, Kunstmann JM, Chalas-Boissonas C, Ducot B, Wolf JP. Seminal leukocytes and clinical outcomes with donor sperm insemination. Fertil Steril 2011, 96:1320-1324 e1321. 10.1016/j.fertnstert.2011.08.025, 21982729.
52. Zorn B, Vidmar G, Meden-Vrtovec H. Seminal reactive oxygen species as predictors of fertilization, embryo quality and pregnancy rates after conventional in vitro fertilization and intracytoplasmic sperm injection. Int J Androl 2003, 26:279-285. 10.1046/j.1365-2605.2003.00424.x, 14511216.
53. Attaran M, Pasqualotto E, Falcone T, Goldberg JM, Miller KF, Agarwal A, Sharma RK. The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization. Int J Fertil Womens Med 2000, 45:314-320.
54. Villegas J, Kehr K, Soto L, Henkel R, Miska W, Sanchez R. Reactive oxygen species induce reversible capacitation in human spermatozoa. Andrologia 2003, 35:227-232. 10.1046/j.1439-0272.2003.00564.x, 12950407.
55. Rivlin J, Mendel J, Rubinstein S, Etkovitz N, Breitbart H. Role of hydrogen peroxide in sperm capacitation and acrosome reaction. Biol Reprod 2004, 70:518-522. 10.1095/biolreprod.103.020487, 14561655.
56. Ford WC. Regulation of sperm function by reactive oxygen species. Hum Reprod update 2004, 10:387-399. 10.1093/humupd/dmh034, 15218008.
57. De Jonge C. Biological basis for human capacitation. Hum Reprod update 2005, 11:205-214. 10.1093/humupd/dmi010, 15817522.
58. Franco JG, Mauri AL, Petersen CG, Massaro FC, Silva LF, Felipe V, Cavagna M, Pontes A, Baruffi RL, Oliveira JB, Vagnini LD. Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl 2012, 35:46-51. 10.1111/j.1365-2605.2011.01154.x, 21535011.