Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Journal of Assisted Reproduction and Genetics

Volumn 26, Issue 2-3, 2009, Pages 151-157

  Fariello, Roberta Maria ^a   Del Giudice, Paula Toni ^a   Spaine, Deborah Montagnini ^a   Fraietta, Renato ^a   Bertolla, Ricardo Pimenta ^a   Cedenho, Agnaldo Pereira ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

DNA damage; Leukocytospermia; Mitochondria metabolism; Semen processing; Sperm

Indexed keywords

CELL NUCLEUS DNA; DNA FRAGMENT;

ADULT; ARTICLE; CLINICAL ARTICLE; COLORIMETRY; COMET ASSAY; CONTROLLED STUDY; DENSITY GRADIENT; HUMAN; HUMAN CELL; LEUKOCYTE; LEUKOCYTOSPERMIA; MALE; MITOCHONDRION; PRIORITY JOURNAL; SEMEN ABNORMALITY; SPERMATOZOON MOTILITY;

ADULT; CENTRIFUGATION, DENSITY GRADIENT; COMET ASSAY; DNA; DNA FRAGMENTATION; HUMANS; INFERTILITY, MALE; LEUKOCYTES; MALE; MIDDLE AGED; MITOCHONDRIA; OXIDATIVE STRESS; SEMEN; SEMEN ANALYSIS; SPECIMEN HANDLING; SPERMATOZOA;

EID: 62449125901     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10815-008-9288-0     Document Type: Article

References (40)

1. World Health Organization. Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. New York: Cambridge University Press; 1999.
2. RJ Aitken JS Clarkson 1987 Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa J Reprod Fertil 81 2 459 469
3. H Wolff 1995 The biologic significance of white blood cells in semen Fertil Steril 63 6 1143 1157
4. JG Alvarez JC Touchstone L Blasco BT Storey 1987 Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity J Androl 8 5 338 348
5. A Iwasaki C Gagnon 1992 Formation of reactive oxygen species in spermatozoa of infertile patients Fertil Steril 57 2 409 416
6. FH Comhaire AM Mahmoud CE Depuydt AA Zalata AB Christophe 1999 Mechanisms and effects of male genital tract infection on sperm quality and fertilizing potential: the andrologist's viewpoint Hum Reprod Update 5 5 393 398
7. E de Lamirande C Gagnon 1993 A positive role for the superoxide anion in triggering hyperactivation and capacitation of human spermatozoa Int J Androl 16 1 21 25
8. E de Lamirande C Gagnon 1995 Capacitation-associated production of superoxide anion by human spermatozoa Free Radic Biol Med 18 3 487 495
9. J Aitken H Fisher 1994 Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk Bioessays 16 4 259 267
10. RK Sharma AE Pasqualotto DR Nelson AJ Thomas Jr A Agarwal 2001 Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic J Androl 22 4 575 583
11. RJ Aitken HW Baker 1995 Seminal leukocytes: passengers, terrorists or good samaritans? Hum Reprod 10 7 1736 1739
12. R Henkel G Maass M Hajimohammad R Menkveld T Stalf J Villegas R Sanchez TF Kruger WB Schill 2003 Urogenital inflammation: changes of leucocytes and ROS Andrologia 35 5 309 313
13. SC Sikka M Rajasekaran WJ Hellstrom 1995 Role of oxidative stress and antioxidants in male infertility J Androl 16 6 464 468
14. JG Alvarez BT Storey 1995 Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa Mol Reprod Dev 42 3 334 346
15. RK Sharma A Agarwal 1996 Role of reactive oxygen species in male infertility Urology 48 6 835 850
16. X Wang RK Sharma A Gupta V George AJ Thomas T Falcone A Agarwal 2003 Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study Fertil Steril 80 844 850
17. H Kodama R Yamaguchi J Fukuda H Kasai T Tanaka 1997 Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients Fertil Steril 68 3 519 524
18. S Chatterjee RG Chowdhury B Khan 2006 Medical management of male infertility J Indian Med Assoc 104 2 74
19. N Mousset-Simeon N Rives L Masse F Chevallier B Mace 2004 Comparison of six density gradient media for selection of cryopreserved donor spermatozoa J Androl 25 6 881 884
20. RR Henkel WB Schill 2003 Sperm preparation for ART Reprod Biol Endocrinol 1 108
21. RJ Aitken JS Clarkson 1988 Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques J Andro 9 6 367 376
22. WCL Ford 1990
23. TF Kruger R Menkveld FS Stander CJ Lombard JP Van der Merwe JA van Zyl K Smith 1986 Sperm morphologic features as a prognostic factor in in vitro fertilization Fertil Steril 46 6 1118 1123
24. Mortimer D. Practical laboratory andrology. New York: Oxford University Press; 1994. p. 57-8.
25. ET Donnelly M O'Connell N McClure SE Lewis 2000 Differences in nuclear DNA fragmentation and mitochondrial integrity of semen and prepared human spermatozoa Hum Reprod 15 7 1552 1561
26. F Hrudka 1987 Cytochemical and ultracytochemical demonstration of cytochrome c oxidase in spermatozoa and dynamics of its changes accompanying ageing or induced by stress Int J Androl 10 6 809 828
27. F Comhaire G Verschraegen L Vermeulen 1980 Diagnosis of accessory gland infection and its possible role in male infertility Int J Androl 3 1 32 45
28. CG Blumer RM Fariello AE Restelli DM Spaine RP Bertolla AP Cedenho 2008 Sperm nuclear DNA fragmentation and mitochondrial activity in men with varicocele Fertil Steril 90 5 1716 1722
29. A Agarwal RA Saleh MA Bedaiwy 2003 Role of reactive oxygen species in the pathophysiology of human reproduction Fertil Steril 79 829 843
30. RM Turner 2003 Tales from the tail: what do we really know about sperm motility? J Androl 24 790 803
31. T Bourgeron 2000 Mitochondrial function and male infertility Results Probl Cell Differ 28 187 210
32. LJ Marnett 1999 Lipid peroxidation-DNA damage by malondialdehyde Mutat Res 424 83 95
33. M Klaude S Eriksson J Nygren G Ahnstrom 1996 The comet assay: mechanisms and technical considerations Mutat Res 363 89 96
34. S Engel T Schreiner R Petzoldt 1999 Lipid peroxidation in human spermatozoa and maintenance of progressive sperm motility Andrologia 31 17 22
35. SE Lewis RJ Aitken 2005 DNA damage to spermatozoa has impacts on fertilization and pregnancy Cell Tissue Res 322 1 33 41
36. MJ Tomlinson CL Barratt ID Cooke 1993 Prospective study of leukocytes and leukocyte subpopulations in semen suggests they are not a cause of male infertility Fertil Steril 60 6 1069 1075
37. S Kaleli F Oçer T Irez E Budak MF Aksu 2000 Does leukocytospermia associate with poor semen parameters and sperm functions in male infertility? The role of different seminal leukocyte concentrations Eur J Obstet Gynecol Reprod Biol 89 2 185 191
38. A Zini V Mak D Phang K Jarvi 1999 Potencial adverse effect of semen processing on human sperm deoxyribonucleic acid integrity Fertil Steril 72 496 499
39. A Zini A Finelli D Phang K Jarvi 2000 Influence of semen processing technique on human sperm DNA integrity Urology 56 1081 1084
40. J Stevanato RP Bertolla V Barradas DM Spaine AP Cedenho V Ortiz 2008 Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates Fertil Steril 90 3 889 890