Direct monitoring of mitochondrial calcium levels in cultured cardiac myocytes using a novel fluorescent indicator protein, GCaMP2-mt

International Journal of Cardiology

Volumn 158, Issue 2, 2012, Pages 225-234

  Iguchi, Moritake ^a   Kato, Masashi ^a   Nakai, Junichi ^b   Takeda, Toshihiro ^a   Matsumoto Ida, Madoka ^a   Kita, Toru ^a   Kimura, Takeshi ^a   Akao, Masaharu ^a  

^a KYOTO UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b SAITAMA UNIVERSITY   (Japan)

Author keywords

Cytosolic calcium; Mitochondrial calcium; Mitochondrial permeability transition pore; Oxidative stress

Indexed keywords

CALCIUM; GCAMP2 PROTEIN; INDICATOR; PROTEIN; TETRAMETHYLRHODAMINE ETHYL ESTER; UNCLASSIFIED DRUG;

ADENOVIRUS; ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CALCIUM CELL LEVEL; CELL DEATH; CELL HETEROGENEITY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; HEART MUSCLE CELL; MEMBRANE POTENTIAL; MITOCHONDRIAL PERMEABILITY; MITOCHONDRION; MONITORING; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; SENSOR; SIGNAL NOISE RATIO;

ANIMALS; ANIMALS, NEWBORN; CALCIUM; CELL DEATH; CELLS, CULTURED; FLUORESCENT DYES; HELA CELLS; HUMANS; MITOCHONDRIA, HEART; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; MYOCYTES, CARDIAC; RATS; RATS, WISTAR;

EID: 84862198234     PISSN: 01675273     EISSN: 18741754     Source Type: Journal    
DOI: 10.1016/j.ijcard.2011.01.034     Document Type: Article

References (37)

1. D.R. Green, and G. Kroemer The pathophysiology of mitochondrial cell death Science 305 2004 626 629
2. G. Kroemer, and J.C. Reed Mitochondrial control of cell death Nat Med 6 2000 513 519
3. A.P. Halestrap, S.J. Clarke, and S.A. Javadov Mitochondrial permeability transition pore opening during myocardial reperfusion-a target for cardioprotection Cardiovasc Res 61 2004 372 385
4. J.N. Weiss, P. Korge, H.M. Honda, and P. Ping Role of the mitochondrial permeability transition in myocardial disease Circ Res 93 2003 292 301
5. M. Crompton The mitochondrial permeability transition pore and its role in cell death Biochem J 341 Pt. 2 1999 233 249
6. P. Bernardi Mitochondrial transport of cations: channels, exchangers, and permeability transition Physiol Rev 79 1999 1127 1155
7. A.P. Halestrap What is the mitochondrial permeability transition pore? J Mol Cell Cardiol 46 2009 821 831
8. 2+ signaling Biochim Biophys Acta 1763 2006 442 449
9. S. Orrenius, B. Zhivotovsky, and P. Nicotera Regulation of cell death: the calcium-apoptosis link Nat Rev Mol Cell Biol 4 2003 552 565
10. D.R. Trollinger, W.E. Cascio, and J.J. Lemasters Mitochondrial calcium transients in adult rabbit cardiac myocytes: inhibition by ruthenium red and artifacts caused by lysosomal loading of Ca(2+)-indicating fluorophores Biophys J 79 2000 39 50
11. K. Bianchi, A. Rimessi, A. Prandini, G. Szabadkai, and R. Rizzuto Calcium and mitochondria: mechanisms and functions of a troubled relationship Biochim Biophys Acta 1742 2004 119 131
12. A.E. Palmer, and R.Y. Tsien Measuring calcium signaling using genetically targetable fluorescent indicators Nat Protoc 1 2006 1057 1065
13. 2+ Proc Natl Acad Sci USA 98 2001 3197 3202
14. S. Arnaudeau, W.L. Kelley, J.V. Walsh Jr., and N. Demaurex Mitochondria recycle Ca(2+) to the endoplasmic reticulum and prevent the depletion of neighboring endoplasmic reticulum regions J Biol Chem 276 2001 29430 29439
15. 2+ indicator targeted to mitochondria J Mol Cell Cardiol 46 2009 891 901
16. 2+ sensors into the mitochondrial matrix Cell Calcium 37 2005 129 136
17. 2+ indicator GCaMP2 Proc Natl Acad Sci USA 103 2006 4753 4758
18. J. Nakai, M. Ohkura, and K. Imoto A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein Nat Biotechnol 19 2001 137 141
19. M. Akao, A. Ohler, B. O'Rourke, and E. Marban Mitochondrial ATP-sensitive potassium channels inhibit apoptosis induced by oxidative stress in cardiac cells Circ Res 88 2001 1267 1275
20. O. Griesbeck, G.S. Baird, R.E. Campbell, D.A. Zacharias, and R.Y. Tsien Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications J Biol Chem. 276 2001 29188 29194
21. 2+ responses Science 280 1998 1763 1766
22. M. Akao, B. O'Rourke, Y. Teshima, J. Seharaseyon, and E. Marban Mechanistically distinct steps in the mitochondrial death pathway triggered by oxidative stress in cardiac myocytes Circ Res 92 2003 186 194
23. M.P. Gupta, I.M. Dixon, D. Zhao, and N.S. Dhalla Influence of ruthenium red on rat heart subcellular calcium transport Can J Cardiol 5 1989 55 63
24. 2+ uptake into mitochondria in vitro and in situ in single cardiac myocytes J Biol Chem 273 1998 10223 10231
25. + antiporter Science 326 2009 144 147
26. L. Filippin, P.J. Magalhaes, G. Di Benedetto, M. Colella, and T. Pozzan Stable interactions between mitochondria and endoplasmic reticulum allow rapid accumulation of calcium in a subpopulation of mitochondria J Biol Chem 278 2003 39224 39234
27. 2 J Mol Cell Cardiol 37 2004 63 70
28. 2+ transients during the contractile cycle in adult rabbit cardiac myocytes Biochem Biophys Res Commun 236 1997 738 742
29. D.W. Eley, B. Korecky, H. Fliss, and M. Desilets Calcium homeostasis in rabbit ventricular myocytes. Disruption by hypochlorous acid and restoration by dithiothreitol Circ Res 69 1991 1132 1138
30. + exchange inhibitor, inhibits the mitochondrial death pathway Circulation 108 2003 2275 2281
31. Y. Kirichok, G. Krapivinsky, and D.E. Clapham The mitochondrial calcium uniporter is a highly selective ion channel Nature 427 2004 360 364
32. 2+ transport J Biol Chem 261 1986 16478 16483
33. 2+ channels Circulation 119 2009 2435 2443
34. M. Murgia, C. Giorgi, P. Pinton, and R. Rizzuto Controlling metabolism and cell death: at the heart of mitochondrial calcium signalling J Mol Cell Cardiol 46 2009 781 788
35. M. Matsumoto-Ida, M. Akao, T. Takeda, M. Kato, and T. Kita Real-time 2-photon imaging of mitochondrial function in perfused rat hearts subjected to ischemia/reperfusion Circulation 114 2006 1497 1503
36. M.R. Duchen Roles of mitochondria in health and disease Diabetes 53 Suppl. 1 2004 S96 S102
37. L.G. Shewan, and A.J. Coats Ethics in the authorship and publishing of scientific articles Int J Cardiol 144 2010 1 2