Mitochondrial calcium transients in adult rabbit cardiac myocytes: Inhibition by ruthenium red and artifacts caused by lysosomal loading of Ca2+-indicating fluorophores

Biophysical Journal

Volumn 79, Issue 1, 2000, Pages 39-50

  Trollinger, Donna R ^a,c   Cascio, Wayne E ^b   Lemasters, John J ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CALCIUM ION; FLUORESCENT DYE; RHODAMINE 123; RUTHENIUM RED;

ANIMAL CELL; ARTICLE; ARTIFACT; CALCIUM TRANSPORT; CONTROLLED STUDY; CYTOSOL; ELECTROSTIMULATION; ENDOSOME; FLUORESCENCE; HEART MITOCHONDRION; HEART MUSCLE CELL; LYSOSOME; NONHUMAN; RABBIT; TRANSPORT KINETICS;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0033918278     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(00)76272-2     Document Type: Article

References (46)

1. Achterberg, P. W. 1988. Adenine nucleotides, purine metabolism and myocardial function. In Myocardial Energy Metabolism. J. W. De Jong, editor. Kluwer Academic, Boston. 45-52.
2. Bowser, D. N., T. Minamikawa, P. Nagley, and D. A. Williams. 1998. Role of mitochondria in calcium regulation of spontaneously contracting cardiac muscle cells. Biophys. J. 75:2004-2014.
3. Calviello, G., and M. Chiesi. 1989. Rapid kinetic analysis of the calcium release channels of skeletal sarcoplasmic reticulum: the effect of inhibitors. Biochemistry. 28:1301-1306.
4. Chacon, E., H. Ohata, I. S. Harper, D. R. Trollinger, B. Herman, and J. J. Lemasters. 1996. Mitochondrial free calcium transients during excitation-contraction in rabbit cardiac myocytes. FEBS Lett. 382:31-36.
5. 2+, and cell volume inside cultured adult rabbit cardiac myocytes during chemical hypoxia: a multiparameter digitized confocal microscopic study. Biophys. J. 66:942-952.
6. Chamberlain, B. K., P. Volpe, and S. Fleischer. 1984. Inhibition of calcium-induced calcium release from purified cardiac sarcoplasmic reticulum vesicles. J. Biol. Chem. 259:7547-7553.
7. Chiesi, M., R. Schwaller, and G. Calviello. 1988. Inhibition of rapid Ca-release from isolated skeletal and cardiac sarcoplasmic reticulum (SR) membranes. Biochem. Biophys. Res. Commun. 154:1-8.
8. Di Lisa, F., G. Gambassi, H. Spurgeon, and R. G. Hansford. 1993. Intramitochondrial free calcium in cardiac myocytes in relation to dehydrogenase activation. Cardiovasc. Res. 27:1840-1844.
9. Gallitelli, M. F., M. Schultz, G. Isenberg, and F. Rudolf. 1999. Twitch-potentiation increases calcium in peripheral more than in central mitochondria of guinea-pig ventricular myocytes. J. Physiol. (Lond.). 518(Part 2):433-447.
10. 2+ stores in sarcoplasmic and endoplasmic reticulum. Science. 275:1643-1648.
11. Griffiths, E. J. 1999. Species dependence of mitochondrial calcium transients during excitation-contraction coupling in isolated cardiomyocytes. Biochem. Biophys. Res. Commun. 263:554-559.
12. Griffiths, E. J., C. J. Ocampo, J. S. Savage, G. A. Rutter, R. G. Hansford, M. D. Stern, and H. S. Silverman. 1998. Mitochondrial calcium transporting pathways during hypoxia and reoxygenation in single rat cardiomyocytes. Cardiovasc. Res. 39:423-433.
13. Griffiths, E. J., M. D. Stern, and H. S. Silverman. 1997. Measurement of mitochondrial calcium in single living cardiomyocytes by selective removal of cytosolic indo 1. Am J. Physiol. 273:C37-C44.
14. 2+ transients. Biochim. Biophys. Acta. 1366:5-15.
15. Gunter, T. E., and D. R. Pfeiffer. 1990. Mechanisms by which mitochondria transport calcium. Am. J. Physiol. 258:C755-C768.
16. Hajnoczky, G., L. D. Robb-Gaspers, M. B. Seitz, and A. P. Thomas. 1995. Decoding of cytosolic calcium oscillations in the mitochondria. Cell. 82:415-425.
17. 2+. Biochem. J. 244:159-164.
18. 2+ ions on pyruvate dehydrogenase interconversion in intact rat heart mitochondria. Biochem. J. 194:721-732.
19. Isenberg, G., S. Han, A. Schemer, and M. F. Wendt-Gallitelli. 1993. Changes in mitochondrial calcium concentration during the cardiac contraction cycle. Cardiovasc. Res. 27:1800-1809.
20. 2+ concentration in single cultured rat brain astrocytes. J. Physiol. (Lond). 497:299-308.
21. Leisey, J. R., L. W. Grotyohann, D. A. Scott, and R. C. Scaduto, Jr. 1993. Regulation of cardiac mitochondrial calcium by average extramitochondrial calcium. Am. J. Physiol. 265:H1203-H1208.
22. Mannella, C. A., K. Buttle, B. K. Rath, and M. Marko. 1998. Electron microscopic tomography of rat-liver mitochondria and their interaction with the endoplasmic reticulum. Biofactors. 8:225-228.
23. 2+ uptake into mitochondria in vitro and in situ in single cardiac myocytes. J. Biol. Chem. 273:10223-10231.
24. McCormack, J. G., and R. M. Denton. 1979. The effects of calcium ions and adenine nucleotides on the activity of pig heart 2-oxoglutarate dehydrogenase complex. Biochem. J. 180:533-544.
25. Miyata, H., E. G. Lakatta, M. D. Stern, and H. S. Silverman. 1992. Relation of mitochondrial and cytosolic free calcium to cardiac myocyte recovery after exposure to anoxia. Circ. Res. 71:605-613.
26. 2+ contraction in living single rat cardiac myocytes. Am. J. Physiol. 261: H1123-H1134.
27. 2+ transport by ruthenium red. Biochem. Biophys. Res. Commun. 42:298-305.
28. Moravec, C. S., and M. Bond. 1991. Calcium is released from the junctional sarcoplasmic reticulum during cardiac muscle contraction. Am. J. Physiol. 260:H989-H987.
29. Moreno-Sanchez, R. 1985a. Contribution of the translocator of adenine nucleotides and the ATP synthase to the control of oxidative phosphorylation and arsenylation in liver mitochondria. J. Biol. Chem. 260: 4028-4034.
30. 2+ concentrations. J. Biol. Chem. 260:12554-12560.
31. Nicchitta, C. V., and J. R. Williamson. 1984. Spermine. A regulator of mitochondrial calcium cycling. J. Biol. Chem. 259:12978-12983.
32. Nicholls, D. G., and M. Crompton. 1980. Mitochondrial calcium transport. FEBS Lett. 111:261-268.
33. Nieminen, A.-L., T. L. Dawson, G. J. Gores, T. Kawanishi, B. Herman, and J. J. Lemasters. 1990. Protection by acidotic pH and fructose against lethal injury to rat hepatocytes from mitochondrial inhibition, ionophores and oxidant chemicals. Biochem. Biophys. Res. Commun. 167: 600-606.
34. Nieminen, A.-L., A. K. Saylor, B. Herman, and J. J. Lemasters. 1994. ATP depletion rather than mitochondrial depolarization mediates hepatocyte killing after metabolic inhibition. Am. J. Physiol. 267:C67-C74.
35. Nieminen, A-L., A. K. Saylor, S. A. Tesfai, B. Herman, and J. J. Lemasters. 1995. Contribution of the mitochondrial permeability transition to lethal injury after exposure of hepatocytes to t-butylhydroperoxide. Biochem. J. 307:99-106.
36. 2+ transients in cardiac myocytes during the excitation-contraction cycle: effects of pacing and hormonal stimulation. J. Bioenerg. Biomembr. 30:207-222.
37. Reed, K. C., and F. L. Bygrave. 1974. The inhibition of mitochondrial calcium transport by lanthanides and ruthenium red. Biochem. J. 140: 143-155.
38. 3-sensitive channels that are sensed by neighboring mitochondria. Science. 262:744-747.
39. 2+ responses. Science. 280:1763-1766.
40. 2+ revealed by specifically targeted recombinant aequorin. Nature. 358:325-332.
41. Robb-Gaspers, L. D., P. Burnett, G. A. Rutter, R. M. Denton, R. Rizzuto, and A. P. Thomas. 1998. Integrating cytosolic calcium signals into mitochondrial metabolic responses. EMBO J. 17:4987-5000.
42. Sparagna, G. C., K. K. Gunter, S. S. Sheu, and T. E. Gunter. 1995. Mitochondrial calcium uptake from physiological-type pulses of calcium. A description of the rapid uptake mode. J. Biol. Chem. 270: 27510-27515.
43. Taegtmeyer, H. 1988. Principles of fuel metabolism in heart muscle. In Myocardial Energy Metabolism. J.W. De Jong, editor. Kluwer Academic, Boston. 17-34.
44. 2+ transients during the contractile cycle in adult rabbit cardiac myocytes. efficient between LysoTracker. Biochem. Biophys. Res. Commun. 236: 738-742.
45. Wendt-Gallitelli, M. F., and G. J. Isenberg. 1991. Total and free myoplasmic calcium during a contraction cycle: x-ray microanalysis in guinea-pig ventricular myocytes. J. Physiol. (Lond.). 435:349-372.
46. Williamson, J. R., and. R. H. Cooper. 1980. Regulation of the citric acid cycle in mammalian systems. FEBS Lett. 117(Suppl.):K73-K85.