A high-throughput, homogeneous, fluorescence resonance energy transfer-based assay for phospho-N-acetylmuramoyl-pentapeptide translocase (MraY)

Journal of Biomolecular Screening

Volumn 17, Issue 5, 2012, Pages 662-672

  Shapiro, Adam B ^a   Jahić, Haris ^a   Gao, Ning ^a   Hajec, Laurel ^a   Rivin, Olga ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

anti infective drugs; enzyme assays or enzyme kinetics; fluorescence methods; high throughput screening

Indexed keywords

ANTIINFECTIVE AGENT; CARRIER PROTEIN; DIMETHYL SULFOXIDE; ENZYME INHIBITOR; KANAMYCIN; MEMBRANE PROTEIN; PEPTIDOGLYCAN; PHOSPHATE BINDING PROTEIN; PHOSPHO N ACETYLMURAMOYLPENTAPEPTIDE TRANSLOCASE; PHOSPHOTRANSFERASE INHIBITOR; TREHALOSE; TRITON X 100; TUNICAMYCIN; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLMURAMIC ACID; URIDINE PHOSPHATE;

ARTICLE; BACTERIAL CELL WALL; BACTERIUM CULTURE; CATALYSIS; CONTROLLED STUDY; DRUG SCREENING; ENERGY TRANSFER; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BINDING; ENZYME KINETICS; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; GENE SEQUENCE; GLYCOPROTEIN SYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; IC 50; MICELLE; NONHUMAN; PRIORITY JOURNAL;

DRUG DISCOVERY; ENZYME ASSAYS; ENZYME INHIBITORS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INHIBITORY CONCENTRATION 50; RECOMBINANT PROTEINS; SMALL MOLECULE LIBRARIES; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

EID: 84861817783     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112436885     Document Type: Article

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