Enhancing IgG purification from serum albumin containing feedstock with hydrophobic charge-induction chromatography

Journal of Chromatography A

Volumn 1244, Issue , 2012, Pages 116-122

  Tong, Hong Fei ^a   Lin, Dong Qiang ^a   Yuan, Xiao Ming ^a   Yao, Shan Jing ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Hydrophobic charge induction chromatography; Immunoglobulin G; PH control; Purification; Salt addition; Serum albumin

Indexed keywords

ANTIBODY PURIFICATION; BOVINE SERUM; BOVINE SERUM ALBUMINS; ELUTION BEHAVIOR; IMMUNOGLOBULIN G; PH CONTROL; PROTEIN-LIGAND INTERACTIONS; SALT ADDITION; SEPARATION SELECTIVITY; SERUM ALBUMIN; WASHING BUFFERS;

ADSORPTION; ANTIBODIES; BODY FLUIDS; FEEDSTOCKS; HYDROPHOBICITY; LOADING; MAMMALS; PURIFICATION; RESINS; SODIUM CHLORIDE;

HYDROPHOBIC CHROMATOGRAPHY;

4 MERCAPTOETHYL PYRIDINE RESIN; ALBUMIN; BUFFER; IMMUNOGLOBULIN G; LIGAND; RESIN; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ADSORPTION; ANALYTICAL PARAMETERS; ANTIGEN BINDING; ANTIGEN PURIFICATION; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DESORPTION; DRUG IMPURITY; ELUTION; FEEDSTOCK; HYDROPHOBIC CHARGE INDUCTION CHROMATOGRAPHY; MATERIALS; PH; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN LIGAND INTERACTION; SALT ADDITION; SCALE UP; STATIC ELECTRICITY;

ADSORPTION; ANIMALS; CATTLE; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; IMMUNOGLOBULIN G; SERUM ALBUMIN, BOVINE; SODIUM CHLORIDE; SODIUM HYDROXIDE;

BOVINAE;

EID: 84861758283     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2012.04.073     Document Type: Article

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