New method for the selective capture of antibodies under physiological conditions

Bioseparation

Volumn 9, Issue 4, 2000, Pages 211-221

  Guerrier, L ^a   Girot, P ^a   Schwartz, W ^b   Boschetti, E ^a  

^a Life Technologies   (France)

^b Life Technologies Inc   (United States)

Author keywords

4 mercaptoethyl pyridine; Antibodies; Capture; Hydrophobic charge induction chromatography; Immunoglobulins

Indexed keywords

ADSORPTION; BIOSEPARATION; CELL CULTURE; DESORPTION; HYBRIDOMA; HYDROPHOBIC CHARGE INDUCTION CHROMATOGRAPHY; HYDROPHOBICITY; LIGAND BINDING; MOLECULAR INTERACTION; PH;

ANIMALS; ANTIBODIES; ANTIBODIES, MONOCLONAL; ASCITIC FLUID; BUFFERS; CATTLE; CHROMATOGRAPHY; CULTURE MEDIA; CULTURE MEDIA, SERUM-FREE; HUMANS; HYBRIDOMAS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; LIGANDS; MICE;

BOVINAE;

EID: 0034468019     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008170226665     Document Type: Article

References (23)

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5. Investigations on the specificity of thiophilic interaction for monoclonal antibodies of different subclasses
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8. Design, synthesis and application of a Protein A mimetic
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14. Process-scale purification from cell culture supernatants: Monoclonal antibodies
15. Affinity purification of immunoglobulin M using a novel synthetic ligand
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18. A simplified procedure for the isolation of immunoglobulins from human serum using a novel type of thiophilic gel at low salt concentration
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20. Five-membered mercaptoheterocyclic ligands for thiophilic adsorption chromatography
21. Application of phase partitioning and thiophilic adsorption chromatography to the purification of monoclonal antibodies from cell culture fluids
22. Isolation of monoclonal antibodies from cell containing hybridoma broth using a Protein A coated adsorbent in expanded beds