Increased thermostability of microbial transglutaminase by combination of several hot spots evolved by random and saturation mutagenesis

Amino Acids

Volumn 42, Issue 2-3, 2012, Pages 987-996

  Buettner, Karin ^a   Hertel, Thomas C ^a   Pietzsch, Markus ^a  

^a Department of Downstream Processing   (Germany)

Author keywords

DNA shuffling; Microbial transglutaminase; Saturation mutagenesis; Thermostability

Indexed keywords

PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; DISPASE; HISTIDINE; PROTEINASE; PROTEINASE K;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DNA SHUFFLING; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME STABILITY; ESCHERICHIA COLI; GENE SEQUENCE; GENETIC RECOMBINATION; GENETIC SCREENING; GENETIC VARIABILITY; HALF LIFE TIME; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; RESIDUE ANALYSIS; STREPTOMYCES; STREPTOMYCES MOBARAENSIS; THERMOSTABILITY; WILD TYPE; AFFINITY CHROMATOGRAPHY; ENZYME ACTIVATION; ENZYMOLOGY; MASS SPECTROMETRY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

STREPTOMYCES MOBARAENSIS;

CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASE K; ENDOPEPTIDASES; ENZYME ACTIVATION; ENZYME STABILITY; HISTIDINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STREPTOMYCES; TRANSGLUTAMINASES;

EID: 84861657293     PISSN: 09394451     EISSN: 14382199     Source Type: Journal    
DOI: 10.1007/s00726-011-1015-y     Document Type: Article

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