Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants

Nature Protocols

Volumn 1, Issue 4, 2006, Pages 2019-2025

  Sparkes, Imogen A ^a   Runions, John ^a   Kearns, Anne ^a   Hawes, Chris ^a  

^a OXFORD BROOKES UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL FUSION; CELL TRANSFORMATION; CULTURE TECHNIQUE; ENDOPLASMIC RETICULUM; GENE EXPRESSION; GOLGI COMPLEX; IN VIVO STUDY; NONHUMAN; PEROXISOME; PLANT LEAF; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN METABOLISM; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; RHIZOBIUM; TRANSGENE;

CLONING, MOLECULAR; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; RECOMBINANT FUSION PROTEINS; RHIZOBIUM; TISSUE CULTURE TECHNIQUES; TOBACCO;

NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 34248653184     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.286     Document Type: Article

References (22)

1. Lippincott-Schwartz, J. & Patterson, G.H. Development and use of fluorescent protein markers in living cells. Science 300, 87-91 (2003).
2. Chapman, S., Oparka, K.J. & Roberts, A.G. New tools for in vivo fluorescence tagging. Curr. Opin. Plant Biol. 8, 565-573 (2005).
3. Lukyanov, K.A., Chudakov, D.M., Lukyanov, S. & Verkhusha, V.V. Photoactivatable fluorescent proteins. Nat. Rev. Mol. Cell Biol. 6, 885-890 (2005).
4. Runions, J., Hawes, C. & Kurup, S. Fluorescent protein fusions for protein localization in plants. in Methods in Molecular Biology: Protein Targeting Protocols (ed. van der Giezen, M.) Humana Press, New Jersey, USA, in the press.
5. Runions, J., Brach, T., Kühner, S. & Hawes, C. Photoactivation of GFP reveals protein dynamics within the endoplasmic reticulum. J. Exp. Bot. 57, 43-50 (2006).
6. Hawes, C. Cell biology of the plant Golgi apparatus. New Phytol. 165, 29-44 (2005).
7. Brandizzi, F., Irons, S.L., Johansen, J., Kotzer, A. & Neumann, U. GFP is the way to glow: Bioimaging of the plant endomembrane system. J. Microsc. 214, 138-158 (2004).
8. Kotzer, A.M. et al. AtRabF2b (Ara7) acts on the vacuolar trafficking pathway in tobacco leaf epidermal cells. J. Cell Sci. 117, 6377-6389 (2004).
9. Sparkes, I.A., Hawes, C. & Baker, A. AtPEX2 and AtPEX10 are targeted to peroxisomes independently of known endoplasmic reticulum trafficking routes. Plant Physiol. 139, 690-700 (2005).
10. Irons, S.L., Evans, D.E. & Brandizzi, F. The first 238 amino acids of the human lamin B receptor are targeted to the nuclear envelop in plants. J. Exp. Bot. 54, 943-950 (2003).
11. Walter, M. et al. Visualisation of protein interactions in living plants cells using bimolecular fluorescence complementation. Plant J. 40, 428-438.
12. daSilva, L.L.P. et al. ER export sites and Golgi bodies behave as single mobile secretory units in plant cells. Plant Cell 16, 1753-1771 (2004).
13. Clough, S.J. & Bent, A.F. Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735-743 (1998).
14. Zhang, X., Henriques, R., Lin, S.-S., Niu, Q.-W. & Chua, N.-H. Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat. Protocols 1, 641-646 (2006).
15. Valvekens, D., van Montagu, M. & van Lijsebettens, M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc. Natl. Acad. Sci. USA 85, 5536-5540 (1988).
16. Sparkes, I.A. et al. An Arabidopsis pex10 null mutant is embryo lethal, implicating peroxisomes in an essential role during plant embryogenesis. Plant Physiol. 133, 1809-1819 (2003).
17. Earley, K.W. et al. Gateway-compatible vectors for plant functional genomics and proteomics. Plant J. 45, 616-629 (2006).
18. Hellen, R., Mullineaux, P. & Klee, H. Technical focus: A guide to Agrobacterium binary Ti vectors. Trends Plant Sci. 5, 446-451 (2000).
19. Wroblewski, T., Tomczak, A. & Michelmore, R. Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol. J. 3, 259-273 (2005).
20. Hofgen, R. & Willmitzer, L. Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res. 16, 9877 (1988).
21. Evans, D.E., Coleman, J.O.D. & Kearns, A. Plant Cell culture. BIOS Scientific Publishers, London, (2003).
22. Boevink, P. et al. Stacks on tracks: The plant Golgi apparatus traffics on an actin/ ER network. Plant J. 15, 441-447 (1998).