High-throughput vectors for efficient gene silencing in plants

Functional Plant Biology

Volumn 29, Issue 10, 2002, Pages 1217-1225

  Helliwell, Chris A ^a   Varsha Wesley, S ^a   Wielopolska, Anna J ^a   Waterhouse, Peter M ^a  

^a CSIRO   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; MUTAGENESIS; POLYMERS; RNA;

PHENOTYPE;

PLANTS (BOTANY);

GENE;

ARABIDOPSIS; EMBRYOPHYTA; EUKARYOTA;

EID: 0036028302     PISSN: 14454408     EISSN: None     Source Type: Journal    
DOI: 10.1071/FP02033     Document Type: Article

References (21)

1. Abbott JC, Barakate A, Pinçon G, Legrand M, Lapierre C, Mila I, Schuch W, Halpin C (2001) Simultaneous suppression of multiple genes by single transgenes. Down-regulation of three unrelated lignin biosynthesis genes in tobacco. Plant Physiology 128, 844-853.
2. Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR (1999) EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science 284, 2148-2152.
3. Burn JE, Smyth DR, Peacock WJ, Dennis ES (1993) Genes conferring late flowering in Arabidopsis thaliana. Genetica 90, 147-155.
4. Chuang CF, Meyerowitz EM (2000) Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proceedings of the National Academy of Sciences USA 97, 4985-4990.
5. Clough SJ, Bent AF (1998) Floral dip: A simplified method for Agrobacterim-mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735-743.
6. Ferrandiz C, Gu Q, Martienssen R, Yanofsky MF (2000) Redundant regulation of meristem identity and plant architecture by Fruitful, Apetela1 and Cauliflower. Development 127, 725-734.
7. Gleave AP (1992) A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Molecular Biology 20, 1203-1207.
8. Hedden P, Phillips A (2000) Gibberellin metabolism: New insights revealed by the genes. Trends in Plant Science 5, 523-530.
9. Helliwell CA, Chandler PM, Poole A, Dennis ES, Peacock WJ (2001) The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the giberellin biosynthesis pathway. Proceedings of the National Academy of Sciences USA 98, 2065-2070.
10. Krysan PJ, Young JC, Sussman MR (1999) T-DNA as an insertional mutagen in Arabidopsis. The Plant Cell 11, 2283-2290.
11. Levin JZ, Framond AJ, Tuttle A, Bauer MW, Heifetz PB (2000) Methods of double-stranded RNA-mediated gene inactivation in Arabidopsis and their use to define an essential gene in methionine biosynthesis. Plant Molecular Biology 44, 759-775.
12. Parinov S, Sevugan M, Ye D, Yang W-C, Kumaran M, Sundaresan V (1999) Analysis of flanking sequences from Dissociation insertion lines: A database for reverse genetics in Arabidopsis. The Plant Cell 11, 2263-2270.
13. Pelaz S, Ditta GS, Baumann E, Wisman E, Yanofsky MF (2000) B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405, 200-203.
14. Ratcliff F, Martin-Hernandez AM, Baulcombe DC (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. The Plant Journal 25, 237-245.
15. Sheldon CC, Burn JE, Perez PP, Metzger J, Edwards JA, Peacock WJ, Dennis ES (1999) The FLF MADS box gene: A repressor of flowering in Arabidopsis regulated by vernalization and methylation. The Plant Cell 11, 445-458.
16. Smith NA, Singh SP, Wang M-B, Stoutjesdijk P, Green A, Waterhouse PM (2000) Total silencing by intron-spliced hairpin RNAs. Nature 407, 319-320.
17. Speulman E, Metz PLJ, van Arkel G, Hekkert PTL, Stiekeme WJ, Pereira A (1999) A two-component enhancer-inhibitor mutagenesis system for functional analysis of the Arabidopsis genome. The Plant Cell 11, 1853-1866.
18. The Arabidopsis Genome Initiative (2000) Analysis of the complete genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796-815.
19. Waterhouse PM, Graham MW, Wang M-B (1998) Virus resistance and gene silencing in plants is induced by double stranded RNA. Proceedings of the National Academy of Sciences USA 95, 13959-13964.
20. Waterhouse PM, Wang M-B, Lough T (2001) Gene silencing as an adaptive defence against viruses. Nature 441, 834-842.
21. Wesley SV, Helliwell CA, Smith NA, Wang M-B, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, Waterhouse PM (2001) Construct design for efficient, effective and high throughput gene silencing in plants. The Plant Journal 27, 581-590.