One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants

New Phytologist

Volumn 187, Issue 1, 2010, Pages 240-250

  Xu, Guoyong ^a   Sui, Ning ^a   Tang, Yang ^a   Xie, Ke ^a   Lai, Yizhen ^a   Liu, Yule ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Hairpin RNA; Ligation independent cloning; Plant; RNAi; Vector; Zero background

Indexed keywords

ARABIDOPSIS PROTEIN; BETA GLUCURONIDASE; GLUCOSYLTRANSFERASE; GREEN FLUORESCENT PROTEIN; PLANT RNA; SGT1 PROTEIN, ARABIDOPSIS; GENETIC MARKER;

CLONAL ORGANISM; COLIFORM BACTERIUM; GENE EXPRESSION; GENETIC ANALYSIS; PHYTOCHEMISTRY; PLANT; POLYMERASE CHAIN REACTION;

ARABIDOPSIS; ARTICLE; CHEMISTRY; CONFORMATION; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC MARKER; GENETICS; INTRON; METABOLISM; METHODOLOGY; MICROBIOLOGY; MOLECULAR CLONING; PHENOTYPE; PHYSIOLOGY; RHIZOBIUM; RNA INTERFERENCE; TOBACCO; TRANSGENIC PLANT; PROCEDURES;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; GENETIC MARKERS; GLUCOSYLTRANSFERASES; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; INTRONS; NUCLEIC ACID CONFORMATION; PHENOTYPE; PLANTS, GENETICALLY MODIFIED; RHIZOBIUM; RNA INTERFERENCE; RNA, PLANT; TOBACCO;

ARABIDOPSIS; ESCHERICHIA COLI; NICOTIANA BENTHAMIANA;

EID: 77953178091     PISSN: 0028646X     EISSN: 14698137     Source Type: Journal    
DOI: 10.1111/j.1469-8137.2010.03253.x     Document Type: Article

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