Enhanced viability of corneal epithelial cells for efficient transport/storage using a structurally modified calcium alginate hydrogel

Regenerative Medicine

Volumn 7, Issue 3, 2012, Pages 295-307

  Wright, Bernice ^a   Cave, Richard A ^a   Cook, Joseph P ^a   Khutoryanskiy, Vitaliy V ^a   Mi, Shengli ^a,b   Chen, Bo ^a   Leyland, Martin ^c   Connon, Che J ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b TSINGHUA UNIVERSITY   (China)

^c ROYAL BERKSHIRE HOSPITAL   (United Kingdom)

Author keywords

calcium alginate gel pore size; calcium alginate gel shape; calcium alginate structure; corneal epithelial cell storage; corneal epithelial cell viability; hydroxyethyl cellulose

Indexed keywords

CALCIUM ALGINATE; CYTOKERATIN 14; CYTOKERATIN 3; HYDROXYETHYLCELLULOSE;

ARTICLE; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL ENCAPSULATION; CELL PROLIFERATION; CELL STORAGE; CELL VIABILITY; CHEMICAL COMPOSITION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORNEA EPITHELIUM; CORNEAL EPITHELIAL CELL; CRYOPRESERVATION; EPITHELIUM CELL; GEL; GEL MASS; HUMAN; HUMAN CELL; HYDROGEL; MITOCHONDRION; POROSITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; STORAGE;

ALGINATES; ANIMALS; CATTLE; CELL DIFFERENTIATION; CELL LINE; CELL SURVIVAL; CELLS, IMMOBILIZED; CELLULOSE; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; GLUCURONIC ACID; HEXURONIC ACIDS; HUMANS; HYDROGEL; LIMBUS CORNEAE; MICROSCOPY, ELECTRON, SCANNING; POROSITY; PRESERVATION, BIOLOGICAL; TRANSPORTATION;

EID: 84861308340     PISSN: 17460751     EISSN: 1746076X     Source Type: Journal    
DOI: 10.2217/rme.12.7     Document Type: Article

References (46)

1. Notara M, Alatza A, Gilfillan J et al. In sickness and in health: corneal epithelial stem cell biology, pathology and therapy. Exp. Eye Res. 90(2), 188-195 (2010).
2. Colabelli Gisoldi RA, Pocobelli A, Villani CM, Amato D, Pellegrini G. Evaluation of molecular markers in corneal regeneration by means of autologous cultures of limbal cells and keratoplasty. Cornea 29(7), 715-722 (2010).
3. Pels E, Beele H, Claerhout I. Eye bank issues: II. Preservation techniques: warm versus cold storage. Int. Ophthalmol. 28(3), 155-163 (2008).
4. Pels E, Rijneveld WJ. Organ culture preservation for corneal tissue. Technical and quality aspects. Dev. Ophthalmol. 43, 31-46 (2009).
5. Talbot M, Carrier P, Giasson CJ et al. Autologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstruction. Mol. Vis. 12, 65-75 (2006).
6. Deshpande P, McKean R, Blackwood KA et al. Using poly(lactide-co- glycolide) electrospun scaffolds to deliver cultured epithelial cells to the cornea. Regen. Med. 5(3), 395-401 (2010).
7. Mi S, Chen B, Wright B, Connon CJ. Ex vivo construction of an artificial ocular surface by combination of corneal limbal epithelial cells and a compressed collagen scaffold containing keratocytes. Tissue Eng. Part A 16(6), 2091-2100 (2010).
8. Mi S, Chen B, Wright B, Connon CJ. Plastic compression of a collagen gel forms a much improved scaffold for ocular surface tissue engineering over conventional collagen gels. J. Biomed. Mater. Res. A 95(2), 447-453 (2010).
9. Wikstrom J, Elomaa M, Syvajarvi H et al. Alginate-based microencapsulation of retinal pigment epithelial cell line for cell therapy. Biomaterials 29(7), 869-876 (2008).
10. Capretto L, Mazzitelli S, Luca G, Nastruzzi C. Preparation and characterization of polysaccharidic microbeads by a microfluidic technique: application to the encapsulation of Sertoli cells. Acta Biomater. 6(2), 429-435 (2010).
11. Cook MT, Tzortzis G, Charalampopoulos D, Khutoryanskiy VV. Production and evaluation of dry alginate-chitosan microcapsules as an enteric delivery vehicle for probiotic bacteria. Biomacromolecules 12(7), 2834-2840 (2011).
12. Krol S, del Guerra S, Grupillo M, Diaspro A, Gliozzi A, Marchetti P. Multilayer nanoencapsulation. New approach for immune protection of human pancreatic islets. Nano Lett. 6(9), 1933-1939 (2006).
13. Cabane P, Gac P, Amat J. Allotransplant of microencapsulated parathyroid tissue in severe postsurgical hypoparathyroidism: a case report. Transplant. Proc. 41(9), 3879-3883 (2009).
14. Kintzios S, Yiakoumetis I, Moschopoulos G, Mangana O, Nomikou K, Simonian A. Differential effect of the shape of calcium alginate matrices on the physiology of immobilized neuroblastoma N2a and Vero cells: a comparative study. Biosens. Bioelectron. 23(4), 543-548 (2007).
15. Hunt NC, Shelton RM, Grover L. An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co-culture. Biotechnol. J. 4(5), 730-737 (2009).
16. Li RH, Altreuter DH, Gentile FT. Transport characterization of hydrogel matrices for cell encapsulation. Biotechnol. Bioeng. 50(4), 365-373 (1996).
17. Hwang CM, Sant S, Masaeli M et al. Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering. Biofabrication 2(3), 1-12 (2010).
18. Seo SJ, Choi YJ, Akaike T, Higuchi A, Cho CS. Alginate/galactosylated chitosan/heparin scaffold as a new synthetic extracellular matrix for hepatocytes. Tissue Eng. Part A 12(1), 33-44 (2006).
19. Kuo CK, Ma PX. Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: part 1. Structure, gelation rate and mechanical properties. Biomaterials 22(6), 511-521 (2001).
20. Veriter S, Mergen J, Goebbels RM et al. In vivo selection of biocompatible alginates for islet encapsulation and subcutaneous transplantation. Tissue Eng. Part A 16(5), 1503-1513 (2010).
21. Bidarra SJ, Barrias CC, Barbosa MA, Soares R, Granja PL. Immobilization of human mesenchymal stem cells within RGD-grafted alginate microspheres and assessment of their angiogenic potential. Biomacromolecules 11(8), 1956-1964 (2010).
22. Mazumder MAJ, Burke NAD, Shen F, Potter MA, Stover HDH. Core-cross-linked alginatemicrocapsules for cell encapsulation. Biomacromolecules 10(6), 1365-1373 (2009).
23. Sakai S, Yamaguchi S, Takei T, Kawakami K. Oxidized alginate-cross-linked alginate/ gelatin hydrogel fibers for fabricating tubular constructs with layered smooth muscle cells and endothelial cells in collagen gels. Biomacromolecules 9(7), 2036-2041 (2008).
24. Awad HA, Wickham MQ, Leddy HA, Gimble JM, Guilak F. Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate and gelatin scaffolds. Biomaterials 25(16), 3211-3222 (2004).
25. Kurth T, Hedbom E, Shintani N et al. Chondrogenic potential of human synovial mesenchymal stem cells in alginate. Osteoarthritis Cartilage 15(10), 1178-1189 (2007).
26. Endres M, Wenda N, Woehlecke H et al. Microencapsulation and chondrogenic differentiation of human mesenchymal progenitor cells from subchondral bone marrow in Ca-alginate for cell injection. Acta Biomater. 6(2), 436-444 (2009).
27. Chayosumrit M, Tuch B, Sidhu K. Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Biomaterials 31(3), 505-514 (2010).
28. Wang N, Adams G, Buttery L, Falcone FH, Stolnik S. Alginate encapsulation technology supports embryonic stem cells differentiation into insulin-producing cells. J. Biotechnol. 144(4), 304-312 (2009).
29. Banerjee A, Arha M, Choudhary S et al. The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells. Biomaterials 30(27), 4695-4699 (2009).
30. Yang IH, Kim SH, Kim YH, Sun HJ, Kim SJ, Lee JW. Comparison of phenotypic characterisation between 'alginate bead' and 'pellet' culture systems as chondrogenic differentiation models for human mesenchymal stem cells. Yonsei Med. J. 45(5), 891-900 (2004).
31. Utheim TP, Raeder S, Utheim OA et al. A novel method for preserving cultured limbal epithelial cells. Br. J. Ophthalmol. 91(6), 797-800 (2007).
32. Raeder S, Utheim TP, Otheim OA et al. Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes and apoptosis in cultured corneal epithelium. invest. Ophthalmol. Vis. Sci. 48(12), 5484-5493 (2007).
33. Araki-Sasaki K, Ohashi Y, Sasabe T et al. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest. Ophthalmol. Vis. Sci. 36(3), 614-621 (1995).
34. Chen B, Mi S, Wright B, Connon CJ. Differentiation status of limbal epithelial cells cultured on intact and denuded amniotic membrane before and after air-lifting. Tissue Eng. Part A 16(9), 2721-2729 (2010).
35. Chhatbar M, Meena R, Prasad K, Siddhanta AK. Microwave assisted rapid method for hydrolysis of sodium alginate for M/G ratio determination. Carbohydr. Polym. 76(4), 650-656 (2009).
36. Chandía NP, Matsuhiro B, Vásquez AE. Alginic acids in Lessonia trabeculata: characterization by formic acid hydrolysis and FT-IR spectroscopy. Carbohydr. Polym. 46(1), 81-87 (2001).
37. Slaughter BV, Khurshid SS, Fisher OZ, Khademhosseini A, Peppas NA. Hydrogels in regenerative medicine. Adv. Mater. 21(32-33), 3307-3329 (2009).
38. Ko HF, Sfeir C, Kumta PN. Novel synthesis strategies for natural polymer and composite biomaterials as potential scaffolds for tissue engineering. Philos. Transact. A Math. Phys. Eng. Sci. 368(1917), 1981-1997 (2010).
39. Davis IS, Shachar-Hill B, Curry MR, Kim KS, Pedley TJ, Hill AE. Osmosis in semi-permeable pores: an examination of the basic flow equations based on an experimental and molecular dynamics study. Proc. R. Soc. A 463(2079), 881-896 (2007).
40. Orive G, Tam SK, Pedraz JL, Halle JP. Biocompatibility of alginate poly-l-lysine microcapsules for cell therapy. Biomaterials 27(20), 3691-3700 (2006).
41. Faustini M, Bucco M, Galeati G et al. Boar sperm encapsulation reduces in vitro polyspermy. Reprod. Domest. Anim. 45(2), 359-362 (2010).
42. Tamponnet C, Boisseau S, Lirsac PN et al. Storage and growth of neuroblastoma cells immobilized in calcium-alginate beads. Appl. Microbiol. Biotechnol. 33(4), 442-447 (1990).
43. Sakurai T, Kimura M, Sato M. Temporary developmental arrest after storage of fertilized mouse oocytes at 4 degrees C: effects on embryonic development, maternal mRNA processing and cell cycle. Mol. Hum. Reprod. 11(5), 325-333 (2005).
44. Dontchos BN, Coyle CH, Izzo NJ et al. Optimizing CO2 normalizes pH and enhances chondrocyte viability during cold storage. J. Orthop. Res. 26(5), 643-650 (2008).
45. Horbinski C, Mojesky C, Kyprianou N. Live free or die: tales of homeless (cells) in cancer. Am. J. Pathol. 177(3), 1044-1052 (2010).
46. Komuro A, Hodge DO, Gores GJ, Bourne WM. Cell death during corneal storage at 4°C. Invest. Ophthalmol. Vis. Sci. 40(12), 2827-2832 (1999).