A novel method for preserving cultured limbal epithelial cells

British Journal of Ophthalmology

Volumn 91, Issue 6, 2007, Pages 797-800

  Utheim, Tor Paaske ^a   Raeder, Sten ^a   Utheim, Øygunn Aass ^a   Cai, Yiqing ^a   Roald, Borghild ^b   Drolsum, Liv ^a   Lyberg, Torstein ^b   Nicolaissen, Bjørn ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKERATIN 19; PROTEIN P63; VIMENTIN;

AMNION; ARTICLE; CALORIMETRY; CELL CULTURE; CELL MEMBRANE; CELL NUCLEUS; CELL VIABILITY; CORNEA EPITHELIUM; HUMAN; IMMUNOHISTOCHEMISTRY; MICROSCOPY; ORGAN CULTURE TECHNIQUE; PHENOTYPE; PRESERVATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; STEM CELL; TISSUE ENGINEERING;

EID: 34249809129     PISSN: 00071161     EISSN: None     Source Type: Journal    
DOI: 10.1136/bjo.2006.103218     Document Type: Article

References (20)

1. Pellegrini G, Traverso CE, Franzi AT, et al. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 1997;349:990-3.
2. Lee SH, Tseng SC. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol 1997;123:303- 12.
3. Meller D, Pires RT, Tseng SC. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br J Ophthalmol 2002;86:463-71.
4. Hershey FB, Cruickshank CN, Mullins LI. The quantitative reduction of 2,3,5-triphenyl tetrazolium chloride by skin in vitro. J Histochem Cytochem 1958;6:191-6.
5. Bravo D, Rigley TH, Gibran N, et al. Effect of storage and preservation methods on viability in transplantable human skin allografts. Burns 2000;26:367-78.
6. Kito K, Kagami H, Kobayashi C, et al. Effects of cryopreservation on histology and viability of cultured corneal epithelial cell sheets in rabbit. Cornea 2005;24:735-41.
7. James SE, Rowe A, Ilari L, et al. The potential for eye bank limbal rings to generate cultured corneal epithelial allografts. Cornea 2001;20:488-94.
8. Joseph A, Powell-Richards AO, Shanmuganathan VA, et al. Epithelial cell characteristics of cultured human limbal explants. Br J Ophthalmol 2004;88:393-8.
9. Shanmuganathan VA, Rotchford AP, Tullo AB, et al. Epithelial proliferative potential of organ cultured corneoscleral rims; implications for allo-limbal transplantation and eye banking. Br J Ophthalmol 2006;90:55-8.
10. Armitage WJ, Easty DL. Factors influencing the suitability of organ-cultured corneas for transplantation. Invest Ophthalmol Vis Sci 1997;38:16-24.
11. Summerlin WT, Miller GE, Harris JE, et al. The organ-cultured cornea: an in vitro study. Invest Ophthalmol 1973;12:176-80.
12. Sachs U, Goldman K, Volenti J, et al. Corneal storage at room temperature. Arch Ophthalmol 1978;96:1075-7.
13. Tamaki K, Varnell ED, Kaufman HE. K-Sol corneal preservation at room temperature. Br J Ophthalmol 1988;72:370-6.
14. Reim M, Hesse R, Pietruschka G. The metabolism of organ cultures of cornea in TC 199 with added dextran 500 or hydroxyethyl starch 450. Klin Monatsbl Augenheilkd 1990;196:76-80.
15. Sandboe FD, Medin W, Froslie KF. Influence of temperature on corneas stored in culture medium. A comparative study using functional and morphological methods. Acta Ophthalmol Scand 2003;81:54-9.
16. Borderie VM, Kantelip BM, Delbosc BY, et al. Morphology, histology, and ultrastructure of human C31 organ-cultured corneas. Cornea 1995;14:300-10.
17. Van Horn DL, Doughman DJ, Harris JE, et al. Ultrastructure of human organ-cultured cornea. II. Stroma and epithelium. Arch Ophthalmol 1975;93:275-7.
18. Grueterich M, Espana E, Tseng SC. Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane. Invest Ophthalmol Vis Sci 2002;43:63-71.
19. Hernandez Galindo EE, Theiss C, Steuhl KP, et al. Gap junctional communication in microinjected human limbal and peripheral corneal epithelial cells cultured on intact amniotic membrane. Exp Eye Res 2003;76:303-14.
20. Ban Y, Cooper U, Fullwood NJ, et al. Comparison of ultrastructure, tight junction-related protein expression and barrier function of human corneal epithelial cells cultivated on amniotic membrane with and without air-lifting. Exp Eye Res 2003;76:735-43.