Functional cell surface display and controlled secretion of diverse agarolytic enzymes by Escherichia coli with a novel ligation-independent cloning vector based on the autotransporter YfaL

Applied and Environmental Microbiology

Volumn 78, Issue 9, 2012, Pages 3051-3058

  Ko, Hyeok Jin ^a   Park, Eunhye ^a   Song, Joseph ^a   Yang, Taek Ho ^b   Lee, Hee Jong ^b   Kim, Kyoung Heon ^a   Choi, In Geol ^a  

^a Korea University   (South Korea)

^b GS Caltex Corporation   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE DISPLAYS; CELL SURFACES; CLEAVAGE SITES; CLONING VECTORS; CONTROLLED RELEASE; CRITICAL REGION; DETRIMENTAL EFFECTS; DIFFERENT SIZES; DISPLAY SYSTEM; E. COLI; FUNCTIONAL CELLS; HETEROLOGOUS PROTEINS; HOST CELLS; RED FLUORESCENT PROTEINS; REPORTER PROTEIN; SACCHAROPHAGUS DEGRADANS; SINGLE CELLS; SURFACE DISPLAYS; TOBACCO ETCH VIRUS PROTEASE;

CELL MEMBRANES; CLONING; DISPLAY DEVICES; ENZYMES; ESCHERICHIA COLI; PROTEINS; VIRUSES;

SCAFFOLDS (BIOLOGY);

AGARASE; CARRIER PROTEIN; ESCHERICHIA COLI PROTEIN; GLYCOSIDASE; PHOTOPROTEIN; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN; YFAL PROTEIN, E COLI;

CLONE; COLIFORM BACTERIUM; CYTOLOGY; ENZYME ACTIVITY; GENE EXPRESSION; PROTEIN; SECRETION; TOBACCO;

ALTEROMONADACEAE; ARTICLE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; MICROBIAL GENETICS; MOLECULAR WEIGHT; REPORTER GENE; SECRETION;

ALTEROMONADACEAE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; GENETICS, MICROBIAL; GLYCOSIDE HYDROLASES; LUMINESCENT PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR WEIGHT; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; SACCHAROPHAGUS DEGRADANS 2-40; TOBACCO ETCH VIRUS;

EID: 84861177190     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07004-11     Document Type: Article

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