RNA interference targeting cytosolic NADP+-dependent isocitrate dehydrogenase exerts anti-obesity effect in vitro and in vivo

Biochimica et Biophysica Acta - Molecular Basis of Disease

Volumn 1822, Issue 8, 2012, Pages 1181-1188

  Nam, Woo Suk ^a   Park, Kwon Moo ^b   Park, Jeen Woo ^a  

^a Kyungpook National University   (South Korea)

^b Kyungpook National University School of Medicine   (South Korea)

Author keywords

Adipogenesis; Lipogenesis; NADPH; Obesity; RNAi

Indexed keywords

ISOCITRATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ADIPOCYTE; ADIPOGENESIS; ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; DIET; GENE SILENCING; GLUCOSE TRANSPORT; IN VITRO STUDY; IN VIVO STUDY; LIPOGENESIS; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN EXPRESSION; RNA INTERFERENCE; TRIACYLGLYCEROL BLOOD LEVEL; WEIGHT REDUCTION;

3T3-L1 CELLS; ADIPOGENESIS; ANIMALS; GENE KNOCKDOWN TECHNIQUES; HYPERLIPIDEMIAS; ISOCITRATE DEHYDROGENASE; MALE; MICE; MICE, INBRED BALB C; MICE, OBESE; MICE, TRANSGENIC; NADP; OBESITY; REACTIVE OXYGEN SPECIES; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

MUS;

EID: 84861120128     PISSN: 09254439     EISSN: 1879260X     Source Type: Journal    
DOI: 10.1016/j.bbadis.2012.04.003     Document Type: Article

References (36)

1. Kopelman P.G. Obesity as a medical problem. Nature 1994, 404:635-643.
2. Kahn B.B., Flier J.S. Obesity and insulin resistance. J. Clin. Invest. 2000, 106:473-481.
3. Spiegelman B.M., Flier J.S. Obesity and the regulation of energy balance. Cell 2001, 104:531-543.
4. +-dependent isocitrate dehydrogenase plays a key role in lipid metabolism. J. Biol. Chem. 2004, 279:39968-39974.
5. Xu Y., Zhang Z., Hu J., Stillman I.E., Leopold J.A., Handy D.E., Loscalzo J., Stanton R.C. Glucose-6-phosphate dehydrogenase-deficient mice have increased renal oxidative stress and increased albuminuria. FASEB J. 2010, 24:609-616.
6. Salati L.M., Amir-Ahmady B. Dietary regulation of expression of glucose 6-phosphate dehydrogenase. Annu. Rev. Nutr. 2001, 21:121-140.
7. Veech R.L., Eggleston L.V., Krebs H.A. The redox state of free nicotinamide-adenine dinucleotide phosphate in the cytoplasm of rat liver. Biochem. J. 1969, 115:609-619.
8. Gumaa K.A., Novello F., McLean P. The pentose phosphate pathway of glucose metabolism. Hormonal and dietary control of the oxidative and non-oxidative reactions and related enzymes of the cycle in adipose tissue. Biochem. J. 1969, 114:253-264.
9. Persico M.G., Viglietto G., Martini G., Toniolo D., Paonessa G., Moscatelli C., Dono R., Vulliamy T., Luzzatto L., D'Urso M. Isolation of human glucose-6-phosphate dehydrogenase (G6PD) cDNA clones: primary structure of the protein and unusual 5' non-coding region. Nucleic Acids Res. 1986, 14:2511-2522.
10. Pankiewicz A., Sledzinski T., Nogalska A., Swierczynski J. Tissue specific, sex and age-related differences in the 6-phosphogluconate dehydrogenase gene expression. Biochem. Cell Biol. 2003, 35:235-245.
11. Loh K., Deng H., Fukushima A., Cai X., Boivin B., Galic S., Bruce C., Shields B.J., Skiba B., Ooms L.M., Stepto N., Wu B., Mitchell C.A., Tonks N.K., Watt M.J., Febbraio M.A., Crack P.J., Andrikopoulos S., Tiganis T. Reactive oxygen species enhance insulin sensitivity. Cell Metab. 2009, 10:260-272.
12. Lassegue B., Clempus R.E. Vascular NAD(P)H oxidases: specific features, expression, and regulation. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2003, 285:R277-R297.
13. Lambeth J.D. NOX enzymes and the biology of reactive oxygen. Nat. Rev. Immunol. 2004, 4:181-189.
14. +-dependent isocitrate dehydrogenase status modulates oxidative damage to cells. Free Radic. Biol. Med. 2002, 32:1185-1196.
15. Kim Y.M., Zhang Y.H., Zhang M.H., Kattach H., Ratnatunga C., Pillai R., Channon K.M., casadei B. A myocardial Nox2 containing NADPH oxidase contributes to oxidative stress in human atrial fibrillation. Circ. Res. 2005, 97:629-636.
16. Minkenberg I., Ferber E. Lucigenin-dependent chemiluminescence as a new assay for NAD(P)H-oxidase activity in particulate fractions of human polymorphonuclear leukocytes. J. Immunol. 1984, 71:61-67.
17. Zerez C.R., Lee S.J., Tanaka K.R. Spectrophotometric determination of oxidized and reduced pyridine nucleotides in erythrocytes using a single extraction procedure. Anal. Biochem. 1987, 164:367-373.
18. Yamada K., Nakata M., Horimoto N., Saito M., Matsuoka H., Inagaki N. Measurement of glucose uptake and intracellular calcium concentration in single, living pancreatic beta-cells. J. Biol. Chem. 2000, 275:22278-22283.
19. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001, 411:494-498.
20. Furukawa S., Fujita T., Shimabukuro M., Iwaki M., Yamada Y., Nakajima Y., Nakayama O., Makishima M., Matsuda M., Shimomura I. Increased oxidative stress in obesity and its impact on metabolic syndrome. J. Clin. Invest. 2004, 114:1752-1761.
21. Wu Z., Xie Y., Morrison R.F., Bucher N.L., Farmer S.R. PPARgamma induces the insulin-dependent glucose transporter GLUT4 in the absence of C/EBPalpha during the conversion of 3T3 fibroblasts into adipocytes. J. Clin. Invest. 1998, 101:22-32.
22. Lee J.H., Park J.W. Oxalomalate regulates ionizing radiation-induced apoptosis in mice. Free Radic. Biol. Med. 2007, 42:44-51.
23. 2 and plays an integral role in insulin signal transduction. Mol. Cell. Biol. 2004, 24:1844-1854.
24. Martyn K.D., Frederick L.M., von Loehneysen K., Dinauer M.C., Knaus U.G. Functional analysis of Nox4 reveals unique characteristics compared to other NADPH oxidases. Cell. Signal. 2006, 18:69-82.
25. Ford E.S., Giles W.H., Dietz W.H. Prevalence of the metabolic syndrome among US adults: findings from the third National Health and Nutrition Examination Survey. JAMA 2002, 287:356-359.
26. Cupp J.R., McAlister-Henn L. NAD(+)-dependent isocitrate dehydrogenase. Cloning, nucleotide sequence, and disruption of the IDH2 gene from Saccharomyces cerevisiae. J. Biol. Chem. 1991, 266:22199-22205.
27. +-dependent isocitrate dehydrogenase. J. Biol. Chem. 2001, 276:16168-16176.
28. Yan H., Parsons D.W., Jin G., McLendon R., Rasheed B.A., Yuan W., Kos I., Batinic-Haberle I., Jones S., Riggins G.J., Friedman H., Friedman A., Reardon D., Herndon J., Kinzler K.W., Velculescu V.E., Vogelstein B., Bigner D.D. IDH1 and IDH2 mutations in gliomas. N. Engl. J. Med. 2009, 360:765-773.
29. Someya S., Yu W., Hallows W.C., Xu J., Vann J.M., Leeuwenburgh C., Tanokura M., Denu J.M., Prolla T.A. Sirt3 mediates reduction of oxidative damage and prevention of age-related hearing loss under caloric restriction. Cell 2010, 143:802-812.
30. Ronnebaum S.M., Ilkayeva O., Burgess S.C., Joseph J.W., Lu D., Stevens R.D., Becker T.C., Sherry A.D., Newgard C.B., Jensen M.V. A pyruvate cycling pathway involving cytosolic NADP-dependent isocitrate dehydrogenase regulates glucose-stimulated insulin secretion. J. Biol. Chem. 2006, 281:30593-30602.
31. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001, 414:813-820.
32. Munro A.W., Girvan H.M., McLean K.J. Cytochrome P450-redox partner fusion enzymes. Biochim. Biophys. Acta 2007, 1770:345-359.
33. Leopold J.A., Cap A., Scribner A.W., Stanton R.C., Loscalzo J. Glucose-6-phosphate dehydrogenase deficiency promotes endothelial oxidant stress and decreases endothelial nitric oxide bioavailability. FASEB J. 2001, 15:1771-1783.
34. Leopold J.A., Zhang Y.Y., Scribner A.W., Stanton R.C., Loscalzo J. Glucose-6-phosphate dehydrogenase overexpression decreases endothelial cell oxidant stress and increases bioavailable nitric oxide. Arterioscler. Thromb. Vasc. Biol. 2003, 23:411-417.
35. Horton J.D. Sterol regulatory element-binding proteins: transcriptional activators of lipid synthesis. Biochem. Soc. Trans. 2002, 30:1091-1095.
36. Muller G., Morawietz H. NAD(P)H oxidase and endothelial dysfunction. Horm. Metab. Res. 2009, 41:152-158.