Using hydrogen/deuterium exchange mass spectrometry to study conformational changes in granulocyte colony stimulating factor upon PEGylation

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 3, 2012, Pages 498-504

  Wei, Hui ^a   Ahn, Joomi ^b,c   Yu, Ying Qing ^b   Tymiak, Adrienne ^a   Engen, John R ^c   Chen, Guodong ^a  

^a BRISTOL MYERS SQUIBB   (United States)

^b WATERS CORPORATION   (United States)

^c NORTHEASTERN UNIVERSITY   (United States)

Author keywords

Biopharmaceutical; Comparability; Polyethylene glycol; Posttranslational modification; Protein conformation; Therapeutic protein

Indexed keywords

BIOPHARMACEUTICAL; COMPARABILITY; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN CONFORMATION; THERAPEUTIC PROTEIN;

DATA PROCESSING; DEUTERIUM; MASS SPECTROMETRY; POLYETHYLENE GLYCOLS; PROCESS MONITORING;

PROTEINS;

DEUTERIUM; GRANULOCYTE COLONY STIMULATING FACTOR; MACROGOL DERIVATIVE; PEGYLATED GRANULOCYTE COLONY STIMULATING FACTOR; PEGYLATED GRANULOCYTE COLONY-STIMULATING FACTOR; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN;

ARTICLE; AUTOMATION; CHEMICAL MODIFICATION; COMPARATIVE STUDY; COMPUTER PROGRAM; CONFORMATIONAL TRANSITION; DATA PROCESSING; HYDROGEN DEUTERIUM EXCHANGE MASS SPECTROMETRY; MASS SPECTROMETRY; PEGYLATION; PROTEIN ANALYSIS; PROTEIN CONFORMATION; AMINO ACID SEQUENCE; CHEMISTRY; DEUTERIUM HYDROGEN EXCHANGE; METABOLISM; METHODOLOGY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; DEUTERIUM EXCHANGE MEASUREMENT; GRANULOCYTE COLONY-STIMULATING FACTOR; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; POLYETHYLENE GLYCOLS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 84860591482     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0310-x     Document Type: Article

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