Selective DNA demethylation by fusion of TDG with a sequence-specific DNA-binding domain

Epigenetics

Volumn 7, Issue 4, 2012, Pages 344-349

  Gregory, David J ^a   Mikhaylova, Lyudmila ^b   Fedulov, Alexey V ^b  

^a HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Demethylase; Demethylation; DNA; Gene function; TDG; Transcriptional enhancement

Indexed keywords

GAMMA INTERFERON; INDUCIBLE NITRIC OXIDE SYNTHASE; INTERFERON REGULATORY FACTOR 1; PROSTAGLANDIN SYNTHASE; STAT1 PROTEIN; THYMINE DNA GLYCOSYLASE;

ARTICLE; CONTROLLED STUDY; CPG ISLAND; DEMETHYLATION; DNA BINDING; DNA DEMETHYLATION; DNA METHYLATION; EPIGENETICS; GENE EXPRESSION; GENE INDUCTION; GENE SILENCING; GENETIC TRANSCRIPTION; HUMAN; NONHUMAN; SIGNAL TRANSDUCTION; TRANSCRIPTION INITIATION; UPREGULATION;

EID: 84860589110     PISSN: 15592294     EISSN: 15592308     Source Type: Journal    
DOI: 10.4161/epi.19509     Document Type: Article

References (19)

1. Jin B, Li Y, Robertson KD. DNA methylation: superior or subordinate in the epigenetic hierarchy? Genes Cancer 2011; 2:607-17; PMID:21941617; http://dx.doi.org/10.1177/1947601910393957.
2. Meister GE, Chandrasegaran S, Ostermeier M. Heterodimeric DNA methyltransferases as a platform for creating designer zinc finger methyltransferases for targeted DNA methylation in cells. Nucleic Acids Res 2010; 38:1749-59; PMID:20007601; http://dx.doi.org/10.1093/nar/gkp1126.
3. Wu SC, Zhang Y. Active DNA demethylation: many roads lead to Rome. Nat Rev Mol Cell Biol 2010; 11:607-20; PMID:20683471; http://dx.doi.org/10.1038/nrm2950.
4. Fedulov AV, Kobzik L. Allergy risk is mediated by dendritic cells with congenital epigenetic changes. Am J Respir Cell Mol Biol 2011; 44:285-92; PMID:20118218; http://dx.doi.org/10.1165/rcmb.2009-0400OC.
5. Hollingsworth JW, Maruoka S, Boon K, Garantziotis S, Li Z, Tomfohr J, et al. In utero supplementation with methyl donors enhances allergic airway disease in mice. J Clin Invest 2008; 118:3462-9; PMID:18802477.
6. Zhu B, Zheng Y, Hess D, Angliker H, Schwarz S, Siegmann M, et al. 5-methylcytosine-DNA glycosylase activity is present in a cloned G/T mismatch DNA glycosylase associated with the chicken embryo DNA demethylation complex. Proc Natl Acad Sci USA 2000; 97:5135-9; PMID:10779566; http://dx.doi.org/10.1073/pnas.100107597.
7. Chen ZX, Riggs AD. DNA methylation and demethylation in mammals. J Biol Chem 2011; 286:18347-53; PMID:21454628; http://dx.doi.org/10.1074/jbc.R110.205286.
8. He YF, Li BZ, Li Z, Liu P, Wang Y, Tang Q, et al. Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA. Science 2011; 333:1303-7; PMID:21817016; http://dx.doi.org/10.1126/science.1210944.
9. Cortellino S, Xu J, Sannai M, Moore R, Caretti E, Cigliano A, et al. Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair. Cell 2011; 146:67-79; PMID:21722948; http://dx.doi.org/10.1016/j.cell.2011.06.020.
10. Luscombe NM, Austin SE, Berman HM, Thornton JM. An overview of the structures of protein-DNA complexes. Genome Biol 2000; 1:1; PMID:11104519; http://dx.doi.org/10.1186/gb-2000-1-1-reviews001.
11. Gilmore TD. Introduction to NFkappaB: players, pathways, perspectives. Oncogene 2006; 25:6680-4; PMID:17072321; http://dx.doi.org/10.1038/sj.onc.1209954.
12. Chan GC, Fish JE, Mawji IA, Leung DD, Rachlis AC, Marsden PA. Epigenetic basis for the transcriptional hyporesponsiveness of the human inducible nitric oxide synthase gene in vascular endothelial cells. J Immunol 2005; 175:3846-61; PMID:16148131.
13. Martin E, Nathan C, Xie QW. Role of interferon regulatory factor 1 in induction of nitric oxide synthase. J Exp Med 1994; 180:977-84; PMID:7520478; http://dx.doi.org/10.1084/jem.180.3.977.
14. Bennett MT, Rodgers MT, Hebert AS, Ruslander LE, Eisele L, Drohat AC. Specificity of human thymine DNA glycosylase depends on N-glycosidic bond stability. J Am Chem Soc 2006; 128:12510-9; PMID:16984202; http://dx.doi.org/10.1021/ja0634829.
15. Nabel CS, Kohli RM. Molecular biology. Demystifying DNA demethylation. Science 2011; 333:1229-30; PMID:21885763; http://dx.doi.org/10.1126/science.1211917.
16. Sander JD, Dahlborg EJ, Goodwin MJ, Cade L, Zhang F, Cifuentes D, et al. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nat Methods 2011; 8:67-9; PMID:21151135; http://dx.doi.org/10.1038/nmeth.1542.
17. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Nat Protoc 2009; 4:1471-501; PMID:19798082; http://dx.doi.org/10.1038/nprot.2009.98.
18. Shi Y, Berg JM. A direct comparison of the properties of natural and designed zinc-finger proteins. Chem Biol 1995; 2:83-9; PMID:9383408; http://dx.doi.org/10.1016/1074-5521(95)90280-5.
19. Pan H, Mostoslavsky G, Eruslanov E, Kotton DN, Kramnik I. Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages. J Immunol Methods 2008; 329:31-44; PMID:17967462; http://dx.doi.org/10.1016/j.jim.2007.09.009.