Cytoskeletal re-arrangement in TGF-β1-induced alveolar epithelial-mesenchymal transition studied by atomic force microscopy and high-content analysis

Nanomedicine: Nanotechnology, Biology, and Medicine

Volumn 8, Issue 3, 2012, Pages 355-364

  Buckley, Stephen T ^a   Medina, Carlos ^a   Davies, Anthony M ^a   Ehrhardt, Carsten ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

A549 cell line; Actin fibers; Extracellular matrix; Idiopathic pulmonary fibrosis; Scanning probe microscopy

Indexed keywords

A549 CELLS; ACTIN FIBERS; EXTRACELLULAR MATRICES; IDIOPATHIC PULMONARY FIBROSIS; SCANNING PROBES;

ATOMIC FORCE MICROSCOPY; BIOMECHANICS; CELL CULTURE; DYNAMICS; PROTEINS; STIFFNESS; STIFFNESS MATRIX;

CELLS;

COLLAGEN TYPE 1; F ACTIN; TRANSFORMING GROWTH FACTOR BETA1;

ARTICLE; ATOMIC FORCE MICROSCOPY; CELL MEMBRANE; CELL SHAPE; CELL STIMULATION; CELL STRUCTURE; CELL SURFACE; CELLULAR DISTRIBUTION; CONTENT ANALYSIS; CYTOARCHITECTURE; CYTOSKELETON; EPITHELIAL MESENCHYMAL TRANSITION; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; RIGIDITY;

ACTINS; ANIMALS; CELL LINE; CELL SHAPE; COLLAGEN TYPE I; CYTOSKELETON; EPITHELIAL-MESENCHYMAL TRANSITION; FLUORESCENCE; HUMANS; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, ATOMIC FORCE; PULMONARY ALVEOLI; RATS; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84860395096     PISSN: 15499634     EISSN: 15499642     Source Type: Journal    
DOI: 10.1016/j.nano.2011.06.021     Document Type: Article

References (31)

1. Thannickal V.J., Toews G.B., White E.S., Lynch J.P., Martinez F.J. Mechanisms of pulmonary fibrosis. Annu Rev Med 2004, 55:395-417.
2. Selman M., Pardo A. The epithelial/fibroblastic pathway in the pathogenesis of idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol 2003, 29:S93-S97.
3. Willis B.C., duBois R.M., Borok Z. Epithelial origin of myofibroblasts during fibrosis in the lung. Proc Am Thorac Soc 2006, 3:377-382.
4. Willis B.C., Borok Z. TGF-beta-induced EMT: mechanisms and implications for fibrotic lung disease. Am J Physiol Lung Cell Mol Physiol 2007, 293:L525-L534.
5. Kalluri R., Weinberg R.A. The basics of epithelial-mesenchymal transition. J Clin Invest 2009, 119:1420-1428.
6. Radisky D.C., Kenny P.A., Bissell M.J. Fibrosis and cancer: do myofibroblasts come also from epithelial cells via EMT?. J Cell Biochem 2007, 101:830-839.
7. Stricker J., Falzone T., Gardel M.L. Mechanics of the F-actin cytoskeleton. J Biomech 2010, 43:9-14.
8. Daley W.P., Peters S.B., Larsen M. Extracellular matrix dynamics in development and regenerative medicine. J Cell Sci 2008, 121:255-264.
9. Hay E.D. Extracellular matrix alters epithelial differentiation. Curr Opin Cell Biol 1993, 5:1029-1035.
10. Zeisberg M., Bonner G., Maeshima Y., Colorado P., Müller G.A., Strutz F., et al. Renal fibrosis: collagen composition and assembly regulates epithelial-mesenchymal transdifferentiation. Am J Pathol 2001, 159:1313-1321.
11. Kim K.K., Kugler M.C., Wolters P.J., Robillard L., Galvez M.G., et al. Alveolar epithelial cell mesenchymal transition develops in vivo during pulmonary fibrosis and is regulated by the extracellular matrix. Proc Natl Acad Sci U S A 2006, 103:13180-13185.
12. Shintani Y., Maeda M., Chaika N., Johnson K.R., Wheelock M.J., Collagen I. promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling. Am J Respir Cell Mol Biol 2008, 38:95-104.
13. DeMaio L., Buckley S.T., Banfalvi A., Flodby P., Minoo P., et al. Extracellular matrix (ECM) modulates epithelial-mesenchymal transition (EMT) of alveolar epithelial cells (AEC). Am J Respir Crit Care Med 2010, 181:A1223.
14. Dudek S.M., Garcia J.G. Cytoskeletal regulation of pulmonary vascular permeability. J Appl Physiol 2001, 91:1487-1500.
15. Krouskop T.A., Wheeler T.M., Kallel F., Garra B.S., Hall T. Elastic moduli of breast and prostate tissues under compression. Ultrason Imaging 1998, 20:260-274.
16. Kilpatrick D., Xu C., Vito R., Glagov S. Correlation of mechanical behaviour and MMP-1 presence in human atherosclerotic plaque. J Mech Med Biol 2002, 2:1-7.
17. Tajaddini A., Kilpatrick D.L., Vince D.G. A novel experimental method to estimate stress-strain behavior of intact coronary arteries using intravascular ultrasound (IVUS). J Biomech Eng 2003, 125:120-123.
18. Samani A. PlewesD A method to measure the hyperelastic parameters of ex vivo breast tissue samples. Phys Med Biol 2004, 49:4395-4405.
19. Willis B.C., Liebler J.M., Luby-Phelps K., Nicholson A.G., Crandall E.D., et al. Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-beta1: potential role in idiopathic pulmonary fibrosis. Am J Pathol 2005, 166:1321-1332.
20. Mofrad M.R.K., Kamm R.D. Cytoskeletal mechanics: models and measurements 2006, 1-252. Cambridge University Press, New York.
21. Kasai H., Allen J.T., Mason R.M., Kamimura T., Zhang Z. TGF-beta1 induces human alveolar epithelial to mesenchymal cell transition (EMT). Respir Res 2005, 6:56.
22. Buckley S.T., Medina C., Ehrhardt C. Differential susceptibility to epithelial-mesenchymal transition (EMT) of alveolar, bronchial and intestinal epithelial cells in vitro and the effect of angiotensin II receptor inhibition. Cell Tissue Res 2010, 342:39-51.
23. Crosby L.M., Waters C.M. Epithelial repair mechanisms in the lung. Am J Physiol Lung Cell Mol Physiol 2010, 298:L715-L731.
24. Darling E.M., Zauscher S., Block J.A., Guilak F. A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy: do cell properties reflect metastatic potential?. Biophys J 2007, 92:1784-1791.
25. Tilghman R.W., Parsons J.T. Focal adhesion kinase as a regulator of cell tension in the progression of cancer. Semin Cancer Biol 2008, 18:45-52.
26. Kidoaki S., Matsuda T. Shape-engineered fibroblasts: cell elasticity and actin cytoskeletal features characterized by fluorescence and atomic force microscopy. J Biomed Mater Res A 2007, 81:803-810.
27. Thoelking G., Reiss B., Wegener J., Oberleithner H., Pavenstaedt H., et al. Nanotopography follows force in TGF-beta1 stimulated epithelium. Nanotechnology 2010, 21:265102.
28. Kuznetsova T.G., Starodubtseva M.N., Yegorenkov N.I., Chizhik S.A., Zhdanov R.I. Atomic force microscopy probing of cell elasticity. Micron 2007, 38:824-833.
29. Dimitriadis E.K., Horkay F., Maresca J., Kachar B., Chadwick R.S. Determination of elastic moduli of thin layers of soft material using the atomic force microscope. Biophys J 2002, 82:2798-2810.
30. Oliver W.C., Pharr G.M. An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments. J Mater Res 1992, 7:1564-1583.
31. McPhee G., Dalby M.J., Riehle M., Yin H. Can common adhesion molecules and microtopography affect cellular elasticity? A combined atomic force microscopy and optical study. Med Biol Eng Comput 2010, 48:1043-1053.