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Volumn 36, Issue 1, 2012, Pages 140-149

Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity

Author keywords

EST; Gene expression; Immune related genes; QPCR; Ruditapes decussatus; Ruditapes philippinarum; Vibrio alginolyticus

Indexed keywords

APOPTOTIC PROTEASE ACTIVATING FACTOR 1; GALECTIN; HIGH MOBILITY GROUP B1 PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INHIBITOR OF APOPTOSIS PROTEIN; TOLL LIKE RECEPTOR;

EID: 84860389463     PISSN: 0145305X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dci.2011.06.012     Document Type: Article
Times cited : (50)

References (55)
  • 1
    • 18744382506 scopus 로고    scopus 로고
    • ProtTest, selection of bestfit models of protein evolution
    • Abascal F., Zardoya R., Posada D. ProtTest, selection of bestfit models of protein evolution. Bioinformatics 2005, 21:2104-2105.
    • (2005) Bioinformatics , vol.21 , pp. 2104-2105
    • Abascal, F.1    Zardoya, R.2    Posada, D.3
  • 2
    • 77956680686 scopus 로고    scopus 로고
    • Survivin and IAP proteins in cell-death mechanisms
    • Altieri D.C. Survivin and IAP proteins in cell-death mechanisms. Biochem. J. 2010, 430:199-205.
    • (2010) Biochem. J. , vol.430 , pp. 199-205
    • Altieri, D.C.1
  • 6
  • 7
    • 0034043778 scopus 로고    scopus 로고
    • Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis
    • Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol. Biol. Evol. 2000, 17:540-552.
    • (2000) Mol. Biol. Evol. , vol.17 , pp. 540-552
    • Castresana, J.1
  • 8
    • 79952196607 scopus 로고    scopus 로고
    • Expression level, evolutionary rate, and the cost of expression
    • Cherry J.L. Expression level, evolutionary rate, and the cost of expression. Genome Biol. Evol. 2010, 2:757-769.
    • (2010) Genome Biol. Evol. , vol.2 , pp. 757-769
    • Cherry, J.L.1
  • 9
    • 24644503098 scopus 로고    scopus 로고
    • Blast2GO, a universal tool for annotation, visualization and analysis in functional genomics research
    • Conesa A., Götz S., García-Gómez J.M., Terol J., Talón M., Robles M. Blast2GO, a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 2005, 21:3674-3676.
    • (2005) Bioinformatics , vol.21 , pp. 3674-3676
    • Conesa, A.1    Götz, S.2    García-Gómez, J.M.3    Terol, J.4    Talón, M.5    Robles, M.6
  • 10
    • 77951298887 scopus 로고    scopus 로고
    • Molecular evidence for the existence of lipopolysaccharide-induced TNF-alpha factor (LITAF) and Rel/NF-κB pathways in disk abalone (Haliotis discus discus)
    • De Zoysa M., Nikapitiya C., Oh C., Whang I., Lee J.S., Jung S.J., Choi C.Y., Lee J. Molecular evidence for the existence of lipopolysaccharide-induced TNF-alpha factor (LITAF) and Rel/NF-κB pathways in disk abalone (Haliotis discus discus). Fish Shellfish Immunol. 2010, 28:754-763.
    • (2010) Fish Shellfish Immunol. , vol.28 , pp. 754-763
    • De Zoysa, M.1    Nikapitiya, C.2    Oh, C.3    Whang, I.4    Lee, J.S.5    Jung, S.J.6    Choi, C.Y.7    Lee, J.8
  • 12
    • 0033977618 scopus 로고    scopus 로고
    • Determinants of substitution rates in mammalian genes, expression pattern affects selection intensity but not mutation rate
    • Duret L., Mouchiroud D. Determinants of substitution rates in mammalian genes, expression pattern affects selection intensity but not mutation rate. Mol. Biol. Evol. 2000, 17:68-74.
    • (2000) Mol. Biol. Evol. , vol.17 , pp. 68-74
    • Duret, L.1    Mouchiroud, D.2
  • 13
    • 0036549588 scopus 로고    scopus 로고
    • The significance of changes in high mobility group-1 protein mRNA expression in rats after thermal injury
    • Fang W.H., Yao Y.M., Shi Z.G., Yu Y., Wu Y., Lu L.R., Sheng Z.Y. The significance of changes in high mobility group-1 protein mRNA expression in rats after thermal injury. Shock 2002, 17:329-333.
    • (2002) Shock , vol.17 , pp. 329-333
    • Fang, W.H.1    Yao, Y.M.2    Shi, Z.G.3    Yu, Y.4    Wu, Y.5    Lu, L.R.6    Sheng, Z.Y.7
  • 14
    • 85190489940 scopus 로고    scopus 로고
    • FAO, Cultured aquatic species information programme. R. philippinarum. In: Goulletquer, P. (Ed.), Cultured Aquatic Species Information Programme, FAO Fisheries and Aquaculture Department [online], Rome. Accessed on: 15 January 2011.
    • FAO, 2005. Cultured aquatic species information programme. R. philippinarum. In: Goulletquer, P. (Ed.), Cultured Aquatic Species Information Programme, FAO Fisheries and Aquaculture Department [online], Rome. Accessed on: 15 January 2011. http://www.fao.org/fishery/culturedspecies/Ruditapes_philippinarum/en.
    • (2005)
  • 17
    • 36749021586 scopus 로고    scopus 로고
    • Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus, identification of new antimicrobial peptides
    • Gestal C., Costa M., Figueras A., Novoa B. Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus, identification of new antimicrobial peptides. Gene 2007, 406:134-143.
    • (2007) Gene , vol.406 , pp. 134-143
    • Gestal, C.1    Costa, M.2    Figueras, A.3    Novoa, B.4
  • 19
    • 77954089746 scopus 로고    scopus 로고
    • MgC1q, a novel C1q-domain-containing protein involved in the immune response of Mytilus galloprovincialis
    • Gestal C., Pallavicini A., Venier P., Novoa B., Figueras A. MgC1q, a novel C1q-domain-containing protein involved in the immune response of Mytilus galloprovincialis. Dev. Comp. Immunol. 2010, 34:926-934.
    • (2010) Dev. Comp. Immunol. , vol.34 , pp. 926-934
    • Gestal, C.1    Pallavicini, A.2    Venier, P.3    Novoa, B.4    Figueras, A.5
  • 21
    • 12244309071 scopus 로고    scopus 로고
    • Isolation of V. alginolyticus and Vibrio splendidus from aquacultured carpet shell clam (Ruditapes decussatus) larvae associated with mass mortalities
    • Gómez-León J., Villamil L., Lemos M.L., Novoa B., Figueras A. Isolation of V. alginolyticus and Vibrio splendidus from aquacultured carpet shell clam (Ruditapes decussatus) larvae associated with mass mortalities. Appl. Environ. Micorb. 2005, 71:98-104.
    • (2005) Appl. Environ. Micorb. , vol.71 , pp. 98-104
    • Gómez-León, J.1    Villamil, L.2    Lemos, M.L.3    Novoa, B.4    Figueras, A.5
  • 23
    • 0242578620 scopus 로고    scopus 로고
    • A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood
    • Guindon S., Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst, Biol. 2003, 52:696-704.
    • (2003) Syst, Biol. , vol.52 , pp. 696-704
    • Guindon, S.1    Gascuel, O.2
  • 24
    • 65649115127 scopus 로고    scopus 로고
    • Regulation of HSP27 on NF-kappaB pathway activation may be involved in metastatic hepatocellular carcinoma cells apoptosis
    • Guo K., Kang N.X., Li Y., Sun L., Gan L., Cui F.J., Gao M.D., Liu K.Y. Regulation of HSP27 on NF-kappaB pathway activation may be involved in metastatic hepatocellular carcinoma cells apoptosis. BMC Cancer 2009, 9:100.
    • (2009) BMC Cancer , vol.9 , pp. 100
    • Guo, K.1    Kang, N.X.2    Li, Y.3    Sun, L.4    Gan, L.5    Cui, F.J.6    Gao, M.D.7    Liu, K.Y.8
  • 25
    • 34247890064 scopus 로고    scopus 로고
    • Nafamostat mesilate inhibits the expression of HMGB1 in lipopolysaccharide-induced acute lung injury
    • Hagiwara S., Iwasaka H., Noguchi T. Nafamostat mesilate inhibits the expression of HMGB1 in lipopolysaccharide-induced acute lung injury. J. Anesth. 2007, 21:164-170.
    • (2007) J. Anesth. , vol.21 , pp. 164-170
    • Hagiwara, S.1    Iwasaka, H.2    Noguchi, T.3
  • 26
    • 0032849859 scopus 로고    scopus 로고
    • CAP3, A ADN sequence assembly program
    • Huang X., Madan A. CAP3, A ADN sequence assembly program. Genome Res. 1999, 9:868-877.
    • (1999) Genome Res. , vol.9 , pp. 868-877
    • Huang, X.1    Madan, A.2
  • 27
    • 33847273186 scopus 로고    scopus 로고
    • The Drosophila inhibitor of apoptosis (IAP) DIAP2 is dispensable for cell survival, required for the innate immune response to gram-negative bacterial infection, and can be negatively regulated by the reaper/hid/grim family of IAP-binding apoptosis inducers
    • Huh J.R., Foe I., Muro I., Chen C.H., Seol J.H., Yoo S.J., Guo M., Park J.M., Hay B.A. The Drosophila inhibitor of apoptosis (IAP) DIAP2 is dispensable for cell survival, required for the innate immune response to gram-negative bacterial infection, and can be negatively regulated by the reaper/hid/grim family of IAP-binding apoptosis inducers. J. Biol. Chem. 2007, 282:2056-2068.
    • (2007) J. Biol. Chem. , vol.282 , pp. 2056-2068
    • Huh, J.R.1    Foe, I.2    Muro, I.3    Chen, C.H.4    Seol, J.H.5    Yoo, S.J.6    Guo, M.7    Park, J.M.8    Hay, B.A.9
  • 28
    • 33746283951 scopus 로고    scopus 로고
    • Analysis of EST and lectin expression in hemocytes of Manila clams (Ruditapes phylippinarum) (Bivalvia, Mollusca) infected with P. olseni
    • Kang Y.S., Kim Y.M., Park K.L., Cho S.K., Choi K.S., Cho M. Analysis of EST and lectin expression in hemocytes of Manila clams (Ruditapes phylippinarum) (Bivalvia, Mollusca) infected with P. olseni. Dev. Comp. Immunol. 2006, 30:1119-1131.
    • (2006) Dev. Comp. Immunol. , vol.30 , pp. 1119-1131
    • Kang, Y.S.1    Kim, Y.M.2    Park, K.L.3    Cho, S.K.4    Choi, K.S.5    Cho, M.6
  • 29
    • 46549085873 scopus 로고    scopus 로고
    • Noble tandem-repeat galectin of Manila clam R. philippinarum is induced upon infection with the protozoan parasite P. olseni
    • Kim J.Y., Kim Y.M., Cho S.K., Choi K.S., Cho M. Noble tandem-repeat galectin of Manila clam R. philippinarum is induced upon infection with the protozoan parasite P. olseni. Dev. Comp. Immunol. 2008, 32:1131-1141.
    • (2008) Dev. Comp. Immunol. , vol.32 , pp. 1131-1141
    • Kim, J.Y.1    Kim, Y.M.2    Cho, S.K.3    Choi, K.S.4    Cho, M.5
  • 30
    • 0034623005 scopus 로고    scopus 로고
    • T-Coffee, a novel method for fast and accurate multiple sequence alignment
    • Notredame C., Higgins D.G., Heringa J. T-Coffee, a novel method for fast and accurate multiple sequence alignment. J. Mol. Biol. 2000, 302:205-217.
    • (2000) J. Mol. Biol. , vol.302 , pp. 205-217
    • Notredame, C.1    Higgins, D.G.2    Heringa, J.3
  • 31
    • 0029155874 scopus 로고
    • Role of lectins (C-reactive protein) in defense of marine bivalves against bacteria
    • Olafsen J.A. Role of lectins (C-reactive protein) in defense of marine bivalves against bacteria. Adv. Exp. Med. Biol. 1995, 371A:343-348.
    • (1995) Adv. Exp. Med. Biol. , vol.371 A , pp. 343-348
    • Olafsen, J.A.1
  • 32
    • 0034288184 scopus 로고    scopus 로고
    • Modulation of the chemiluminescence response of Mediterranean mussel (Mytilus gallorpovincialis) haemocytes
    • Ordás M.C., Novoa B., Figueras A. Modulation of the chemiluminescence response of Mediterranean mussel (Mytilus gallorpovincialis) haemocytes. Fish Shellfish Immunol. 2000, 10:611-622.
    • (2000) Fish Shellfish Immunol. , vol.10 , pp. 611-622
    • Ordás, M.C.1    Novoa, B.2    Figueras, A.3
  • 33
    • 0034288742 scopus 로고    scopus 로고
    • Immune parameters in carpet shell clams naturally infected with Perkinsus atlanticus
    • Ordás M.C., Ordás A., Beloso C., Figueras A. Immune parameters in carpet shell clams naturally infected with Perkinsus atlanticus. Fish Shellfish Immunol. 2000, 10:597-609.
    • (2000) Fish Shellfish Immunol. , vol.10 , pp. 597-609
    • Ordás, M.C.1    Ordás, A.2    Beloso, C.3    Figueras, A.4
  • 34
    • 11244351527 scopus 로고    scopus 로고
    • Bacterial disease in marine bivalves, review of recent studies. Trends and evolution
    • Paillard C., Leroux F., Borrego J.J. Bacterial disease in marine bivalves, review of recent studies. Trends and evolution. Aquat. Living Resour. 2004, 17:477-498.
    • (2004) Aquat. Living Resour. , vol.17 , pp. 477-498
    • Paillard, C.1    Leroux, F.2    Borrego, J.J.3
  • 35
    • 36549039976 scopus 로고    scopus 로고
    • Sequence variability of myticins identified in haemocytes from mussels suggests ancient host-pathogen interactions
    • Pallavicini A., Costa M.M., Gestal C., Dreos R., Figueras A., Venier P., Novoa B. Sequence variability of myticins identified in haemocytes from mussels suggests ancient host-pathogen interactions. Dev. Comp. Immunol. 2008, 32:213-226.
    • (2008) Dev. Comp. Immunol. , vol.32 , pp. 213-226
    • Pallavicini, A.1    Costa, M.M.2    Gestal, C.3    Dreos, R.4    Figueras, A.5    Venier, P.6    Novoa, B.7
  • 36
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29:2002-2007.
    • (2001) Nucleic Acids Res. , vol.29 , pp. 2002-2007
    • Pfaffl, M.W.1
  • 37
    • 59349110435 scopus 로고    scopus 로고
    • Differentially expressed genes of the carpet shell clam Ruditapes decussatus against P. olseni
    • Prado-Alvarez M., Gestal C., Novoa B., Figueras A. Differentially expressed genes of the carpet shell clam Ruditapes decussatus against P. olseni. Fish Shellfish Immunol. 2009, 26:72-83.
    • (2009) Fish Shellfish Immunol. , vol.26 , pp. 72-83
    • Prado-Alvarez, M.1    Gestal, C.2    Novoa, B.3    Figueras, A.4
  • 38
    • 60649110212 scopus 로고    scopus 로고
    • Characterization of a C3 and a factor B-like in the carpet-shell clam, Ruditapes decussatus
    • Prado-Alvarez M., Rotllant J., Gestal C., Novoa B., Figueras A. Characterization of a C3 and a factor B-like in the carpet-shell clam, Ruditapes decussatus. Fish Shellfish Immunol. 2009, 26:305-315.
    • (2009) Fish Shellfish Immunol. , vol.26 , pp. 305-315
    • Prado-Alvarez, M.1    Rotllant, J.2    Gestal, C.3    Novoa, B.4    Figueras, A.5
  • 39
    • 33846561574 scopus 로고    scopus 로고
    • Molecular cloning and expression of a toll receptor gene homologue from Zhikong Scallop, C. farreri
    • Qiu L., Song L., Xu W., Ni D., Yu Y. Molecular cloning and expression of a toll receptor gene homologue from Zhikong Scallop, C. farreri. Fish Shellfish Immunol. 2007, 22:451-466.
    • (2007) Fish Shellfish Immunol. , vol.22 , pp. 451-466
    • Qiu, L.1    Song, L.2    Xu, W.3    Ni, D.4    Yu, Y.5
  • 40
    • 79951821141 scopus 로고    scopus 로고
    • New Insights into the apoptotic process in Mollusks: characterization of caspase genes in Mytilus galloprovincialis
    • Romero A., Estévez-Calvar N., Dios S., Figueras A., Novoa B. New Insights into the apoptotic process in Mollusks: characterization of caspase genes in Mytilus galloprovincialis. PLoS One 2011, 6:e17003.
    • (2011) PLoS One , vol.6
    • Romero, A.1    Estévez-Calvar, N.2    Dios, S.3    Figueras, A.4    Novoa, B.5
  • 41
    • 0033990048 scopus 로고    scopus 로고
    • Primer3 on the WWW for general users and for biologist programmers
    • Rozen S., Skaletsky H.J. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 2000, 132:365-386.
    • (2000) Methods Mol. Biol. , vol.132 , pp. 365-386
    • Rozen, S.1    Skaletsky, H.J.2
  • 42
    • 63349103637 scopus 로고    scopus 로고
    • Ferritin functions as a proinflammatory cytokine via iron-independent protein kinase C zeta/nuclear factor kappaB-regulated signaling in rat hepatic stellate cells
    • Ruddell R.G., Hoang-Le D., Barwood J.M., Rutherford P.S., Piva T.J., Watters D.J., Santambrogio P., Arosio P., Ramm G.A. Ferritin functions as a proinflammatory cytokine via iron-independent protein kinase C zeta/nuclear factor kappaB-regulated signaling in rat hepatic stellate cells. Hepatology 2009, 49:887-900.
    • (2009) Hepatology , vol.49 , pp. 887-900
    • Ruddell, R.G.1    Hoang-Le, D.2    Barwood, J.M.3    Rutherford, P.S.4    Piva, T.J.5    Watters, D.J.6    Santambrogio, P.7    Arosio, P.8    Ramm, G.A.9
  • 43
    • 0036401433 scopus 로고    scopus 로고
    • Cytokine expression in three mouse models of experimental hepatitis
    • Sass G., Heinlein S., Agli A., Bang R., Schumann J., Tiegs G. Cytokine expression in three mouse models of experimental hepatitis. Cytokine 2002, 19:115-120.
    • (2002) Cytokine , vol.19 , pp. 115-120
    • Sass, G.1    Heinlein, S.2    Agli, A.3    Bang, R.4    Schumann, J.5    Tiegs, G.6
  • 44
    • 0027168447 scopus 로고
    • NF-kappa B controls expression of inhibitor I kappa B alpha: evidence for an inducible autoregulatory pathway
    • Sun S.C., Ganchi P.A., Ballard D.W., Greene W.C. NF-kappa B controls expression of inhibitor I kappa B alpha: evidence for an inducible autoregulatory pathway. Science 1993, 259:1912-1915.
    • (1993) Science , vol.259 , pp. 1912-1915
    • Sun, S.C.1    Ganchi, P.A.2    Ballard, D.W.3    Greene, W.C.4
  • 45
    • 0037363820 scopus 로고    scopus 로고
    • Nitric oxide production by carpet shell clam (Ruditapes decussatus) hemocytes
    • Tafalla C., Gómez-León J., Novoa B., Figueras A. Nitric oxide production by carpet shell clam (Ruditapes decussatus) hemocytes. Dev. Comp. Immunol. 2003, 27:197-205.
    • (2003) Dev. Comp. Immunol. , vol.27 , pp. 197-205
    • Tafalla, C.1    Gómez-León, J.2    Novoa, B.3    Figueras, A.4
  • 46
    • 77950343791 scopus 로고    scopus 로고
    • Pattern recognition receptors and inflammation
    • Takeuchi O., Akira S. Pattern recognition receptors and inflammation. Cell 2010, 140:805-820.
    • (2010) Cell , vol.140 , pp. 805-820
    • Takeuchi, O.1    Akira, S.2
  • 51
    • 79955527163 scopus 로고    scopus 로고
    • Development of Expressed Sequence Tags from the Pearl Oyster, Pinctada martensii Dunker
    • Wang A., Wang Y., Gu Z., Li S., Shi Y., Guo X. Development of Expressed Sequence Tags from the Pearl Oyster, Pinctada martensii Dunker. Mar. Biotechnol. 2011, 13:275-283.
    • (2011) Mar. Biotechnol. , vol.13 , pp. 275-283
    • Wang, A.1    Wang, Y.2    Gu, Z.3    Li, S.4    Shi, Y.5    Guo, X.6
  • 52
    • 67649503016 scopus 로고    scopus 로고
    • NF-kappaB regulates thrombin-induced ICAM-1 gene expression in cooperation with NFAT by binding to the intronic NF-kappaB site in the ICAM-1 gene
    • Xue J., Thippegowda P.B., Hu G., Bachmaier K., Christman J.W., Malik A.B., Tiruppathi C. NF-kappaB regulates thrombin-induced ICAM-1 gene expression in cooperation with NFAT by binding to the intronic NF-kappaB site in the ICAM-1 gene. Physiol. Genomics 2009, 38:42-53.
    • (2009) Physiol. Genomics , vol.38 , pp. 42-53
    • Xue, J.1    Thippegowda, P.B.2    Hu, G.3    Bachmaier, K.4    Christman, J.W.5    Malik, A.B.6    Tiruppathi, C.7
  • 53
    • 34248582388 scopus 로고    scopus 로고
    • Molecular cloning and characterization of a thioester-containing protein from Zhikong scallop C. farreri
    • Zhang H., Song L., Li C., Zhao J., Wang H., Gao Q., Xu W. Molecular cloning and characterization of a thioester-containing protein from Zhikong scallop C. farreri. Mol. Immunol. 2007, 44:3492-3500.
    • (2007) Mol. Immunol. , vol.44 , pp. 3492-3500
    • Zhang, H.1    Song, L.2    Li, C.3    Zhao, J.4    Wang, H.5    Gao, Q.6    Xu, W.7
  • 54
    • 68949147766 scopus 로고    scopus 로고
    • Molecular characterization and expression analysis of a putative LPS-induced TNF-alpha factor (LITAF) from pearl oyster Pinctada fucata
    • Zhang D., Jiang J., Jiang S., Ma J., Su T., Qiu L., Zhu C., Xu X. Molecular characterization and expression analysis of a putative LPS-induced TNF-alpha factor (LITAF) from pearl oyster Pinctada fucata. Fish Shellfish Immunol. 2009, 27:391-396.
    • (2009) Fish Shellfish Immunol. , vol.27 , pp. 391-396
    • Zhang, D.1    Jiang, J.2    Jiang, S.3    Ma, J.4    Su, T.5    Qiu, L.6    Zhu, C.7    Xu, X.8
  • 55
    • 77953160263 scopus 로고    scopus 로고
    • The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress
    • Zhang L., Wang L., Song L., Zhao J., Qiu L., Dong C., Li F., Zhang H., Yang G. The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress. Mol. Biol. Rep. 2010, 37:1763-1771.
    • (2010) Mol. Biol. Rep. , vol.37 , pp. 1763-1771
    • Zhang, L.1    Wang, L.2    Song, L.3    Zhao, J.4    Qiu, L.5    Dong, C.6    Li, F.7    Zhang, H.8    Yang, G.9


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