The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: Insights into the mechanisms responsible for improved affinity

Journal of Molecular Biology

Volumn 332, Issue 1, 2003, Pages 195-204

  Pal, Gabor ^a,b   Kossiakoff, Anthony A ^a,b   Sidhu, Sachdev S ^c  

^a UNIVERSITY OF CHICAGO   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

^c GENENTECH INC   (United States)

Author keywords

Alanine scanning; Combinatorial mutagenesis; Phage display; Protein protein interactions; Shotgun scanning

Indexed keywords

ALANINE; EPITOPE; GROWTH HORMONE; GROWTH HORMONE RECEPTOR;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; COMBINATORIAL CHEMISTRY; CONTROLLED STUDY; ENERGY; MOLECULAR INTERACTION; MUTAGENESIS; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

EID: 0043237620     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(03)00898-2     Document Type: Article

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