A cellulase gene from a new alkalophilic Bacillus sp. (strain N186-1). Its cloning, nucleotide sequence and expression in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 46, Issue 2, 1996, Pages 149-155

  Sanchez Torres J ^a,c   Perez P ^a   Santamaria R I ^a,b  

^a UNIVERSITY OF SALAMANCA   (Spain)

^b Instituto de Biología Funcional y Genómica and Departamento de Microbiología y Genética   (Spain)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULASE;

ALKALINITY; ARTICLE; BACILLUS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PH; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; CELLULASE; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; SEQUENCE HOMOLOGY, AMINO ACID;

BACILLUS SP.; ESCHERICHIA COLI;

EID: 0029744080     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050797     Document Type: Article

References (37)

1. Baird SD, Hefford MA, Johnson DA, Sung WL, Yaguchi M, Seligy V (1990a) The Glu residue in the conserved Asn-Glu-Pro sequence of two highly divergent endo-β-glucanases is essential for enzymatic activity. Biochem Biophys Res Commun 169: 1035 1039
2. Baird SD, Johnson DA, Seligy V (1990b) Molecular cloning, expression, and characterization of endo-β-1,4 glucanase genes from Bacillus polymyxa and Bacillus circulans. J Bacteriol 172: 1576-1586
3. Béguin P (1983) Detection of cellulase activity in polyacrylamide gels using Congo red-stained replicas. Anal Biochem 131: 333-336
4. Birboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513 1523
5. Fukumori F, Kudo T, Horikoshi K (1985) Purification and properties of a cellulase from alkalophilic Bacillus sp. no. 1139. J Gen Microbiol 131:3339-3345
6. Fukumori F, Kudo T, Narahashi Y, Horikoshi K (1986a) Molecular cloning and nucleotide sequence of the alkaline cellulase gene from the alkalophilic Bacillus sp. strain 1139. J Gen Microbiol 132:2329-2335
7. Fukumori F, Sashihara N, Kudo T, Horikoshi K (1986b) Nucleotide sequence of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology. J Bacteriol 168:479-485
8. Fukumori F, Kudo T, Sashihara N, Nagata Y, Ito K, Horikoshi K (1989) The third cellulase of alkalophilic Bacillus sp. strain N-4: evolutionary relationships within the cel family. Gene 76:289-298
9. Gilkes NR, Henrissat B, Kilburn DG, Miller RC, Warren R (1991) Domains in microbial β-1,4-glycanases: sequence conservation, function and enzyme families. Microb Rev 55:303-315
10. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580
11. Heijne G von (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14:4683-4690
12. Henrissat B (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 280:309-316
13. Henrissat B, Bairoch A (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293:781-788
14. Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73:237-244
15. Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H. (1985) Genetic manipulation of Streptomyces: a laboratory manual. J. I. Foundation, Norwich
16. Horikoshi H, Nakao M, Kurono Y, Sashihara N (1984) Cellulases of an alkalophilic Bacillus strain isolated from soil. Can J Microbiol 30:774-779
17. Ito S, Shikata S, Ozaki K, Kawai S, Okamoto K, Inoue S, Takei A, Ohta Y, Satoh T (1989) Alkaline cellulase for laundry detergents: production by Bacillus sp. KSM-635 and enzymatic properties. Agric Biol Chem 53:1275-1281
18. Janssen GR, Bibb MJ (1993) Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 124:133-134
19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227:680-685
20. Lowry O, Rosebrough A, Farr A, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265 275
21. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Biochem 31:426-428
22. Moran CP, Lang N, Le Grice SFJ, Lee G, Stephen M, Sonenshein AL, Pero J, Losick R (1982) Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet 186:339-346
23. Nakamura A, Uozomi T, Beppu T (1987) Nucleotide sequence of a cellulase gene of Bacillus subtilis. Eur J Biochem 164:317 320
24. Neu HC, Heppel LA (1965) The release of enzymes from E. coli by osmotic shock and during the formation of spheroplasts. J Biol Chem 240:3685-3692
25. Ozaki K, Shikata S, Kawai S, Ito S, Okamoto K (1990) Molecular cloning and nucleotide sequence of a gene for alkaline cellulase from Bacillus sp. KSM-635. J Gen Microbiol 136:1327 1334
26. Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444-2448
27. Pustell J, Kafatos F (1982) A high speed, high capacity similarity matrix: zooming through SV40 and polyoma. Nucleic Acids Res 10:4765-4782
28. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239:487-491
29. Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
30. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467
31. Simonen M, Palva I (1993) Protein secretion in Bacillus species. Microbiol Rev 57:109-137
32. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 184:60-89
33. Tabernero C, Coll PM, Fernández-Abalos JM, Pérez P, Santamaria R (1994) Cloning and DNA sequencing of bgaA, a gene encoding an endo-β-1,3-1,4-glucanase, from an alkalophilic Bacillus strain (N137). Appl Environ Microbiol 60:1213-1220.
34. Teather RM, Wood PJ (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777 780
35. Vieira J, Messing J (1987) Production of single-stranded plasmid DNA. Methods Enzymol 153:3-11
36. 280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-β-1,4-glucanase. J Biol Chem 268:14096-14102
37. Zappe H, Jones Wa, Jones DT, Woods DR (1988) Structure of an endo-β-1,4-glucanase gene from Clostrididium acetobutylicum P262 showing homology with endoglucanase genes from Bacillus spp. Appl Environ Microbiol 54:1289-1292