High-level secretory production of intact, biologically active staphylokinase from Bacillus subtilis

Biotechnology and Bioengineering

Volumn 62, Issue 1, 1999, Pages 87-96

  Ye, Ruiqiong ^a   Kim, June Hyung ^b   Kim, Byung Gee ^b   Szarka, Steven ^a   Sihota, Elaine ^a   Wong, Sui Lam ^a  

^a UNIVERSITY OF CALGARY   (Canada)

^b Seoul National University   (South Korea)

Author keywords

Bacillus subtilis; Fermentation; Secretory production; Staphylokinase; Sucrose induction

Indexed keywords

AMINO ACIDS; BACTERIA; CELL CULTURE; FERMENTATION; GENES; PROTEINS;

BACILLUS SUBTILIS; SECRETORY PRODUCTION; STAPHYLOKINASE;

ENZYMES;

STAPHYLOKINASE;

ARTICLE; BACILLUS SUBTILIS; CORONARY ARTERY THROMBOSIS; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME SYNTHESIS; FIBRINOLYTIC THERAPY; NONHUMAN; THROMBOSIS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0033524383     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0290(19990105)62:1<87::aid-bit10>3.3.co;2-9     Document Type: Article

References (6)

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2. Collen D, De Cock F, Stassen J-M. 1993. Comparative immunogenicity and thrombolytic properties towards arterial and venous thrombi of streptokinase and recombinant staphylokinase in baboons. Circulation 87:996-1006.
3. Collen D, De Cock F, Vanlinthout I, Declerck PJ, Lijnen HR, Stassen JM. 1992. Comparative thrombolytic and immunogenic properties of staphylokinase and streptokinase. Fibrinolysis 6:232-242.
4. Collen D, Lijnen HR. 1994. Staphylokinase, a fibrin-specific plasminogen activator with therapeutic potential. Blood 84:680-686.
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6. Ye R, Yang LP, Wong S-L. 1996. Construction of protease-deficient Bacillus subtilis strains for expression studies: Inactivation of seven extracellular protease and the intracellular LonA protease. Proc. International symposium on recent advances in bioindustry. p 160-169.