Fusion of cellulose binding domain to the catalytic domain improves the activity and conformational stability of chitinase in Bacillus licheniformis DSM13

Bioresource Technology

Volumn 101, Issue 10, 2010, Pages 3635-3641

  Neeraja, Chilukoti ^a,b   Moerschbacher, Bruno ^b   Podile, Appa Rao ^a  

^a UNIVERSITY OF HYDERABAD   (India)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Bacillus licheniformis; CD analysis; Cellulose binding domain; Chitinase

Indexed keywords

BACILLUS LICHENIFORMIS; CATALYTIC DOMAINS; CELLULOSE BINDING DOMAINS; CHITIN BINDING DOMAINS; CHITINASES; COLLOIDAL CHITIN; CONFORMATIONAL STABILITIES; DELETION MUTANTS; N-TERMINALS; SUBSTRATE-BINDING DOMAINS;

BACTERIOLOGY; CELLULOSE; CHITIN; GLYCOLS;

POLYMERASE CHAIN REACTION;

CELLULOSE; CHITIN; CHITINASE; GLYCOL; HYBRID PROTEIN;

BACTERIUM; CATALYSIS; CELLULOSE; CHITIN; POLYMERASE CHAIN REACTION;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS LICHENIFORMIS; BINDING KINETICS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DELETION MUTANT; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME ENGINEERING; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN;

BACILLUS; CATALYTIC DOMAIN; CELLULOSE; CHITINASE; CHROMATOGRAPHY, THIN LAYER; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

BACTERIA; BACTERIOLOGY; BINDING; CELLULOSE; CHITIN; GLYCOLS;

BACILLUS LICHENIFORMIS;

EID: 76049087800     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2009.12.118     Document Type: Article

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