Controlled in meso phase crystallization - a method for the structural investigation of membrane proteins

PLoS ONE

Volumn 7, Issue 4, 2012, Pages

  Kubicek, Jan ^a   Schlesinger, Ramona ^b,c   Baeken, Christian ^b   Büldt, Georg ^b   Schäfer, Frank ^a   Labahn, Jörg ^b  

^a QIAGEN GMBH   (Germany)

^b FORSCHUNGSZENTRUM JÜLICH   (Germany)

^c FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIUM SULFATE; BACTERIORHODOPSIN; HALORHODOPSIN; MEMBRANE PROTEIN; RHODOPSIN; ACYLGLYCEROL; GLYCEROL OLEATE; SENSORY RHODOPSIN; SENSORY RHODOPSIN II PROTEIN, ARCHAEAL;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; HALOBACTERIUM SALINARUM; HYDROPHILICITY; IN MESO CRYSTALLIZATION; NONHUMAN; PH; PROTEIN ANALYSIS; PROTEIN STABILITY; PROTEIN STRUCTURE; CHEMISTRY; METHODOLOGY; PHASE TRANSITION; X RAY CRYSTALLOGRAPHY;

HALOBACTERIUM SALINARUM;

BACTERIORHODOPSINS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; GLYCERIDES; HALOBACTERIUM SALINARUM; HALORHODOPSINS; MEMBRANE PROTEINS; PHASE TRANSITION; PROTEIN STABILITY; SENSORY RHODOPSINS;

EID: 84859808292     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0035458     Document Type: Article

References (33)

1. Benvenuti M, Mangani S, (2007) Crystallization of soluble proteins in vapor diffusion for X-ray crystallography. Nature Protoc 2: 1633-1651.
2. McPherson A, Cudney B, (2006) Searching for silver bullets: An alternative strategy for crystallizing macromolecules. J Struct Biol 156: 387-406.
3. Landau EM, Rosenbusch JP, (1996) Lipidic cubic phases: A novel concept for the crystallization of membrane proteins. Proc Natl Acad Sci USA 93: 14532-14535.
4. Pebay-Peyroula E, Rummel G, Rosenbusch JP, Landau EM, (1997) X-ray structure of BR at 2.5 Angstroms from microcrystals grown in lipidic cubic phases. Science 277: 1676-1681.
5. Qiu H, Caffrey M, (2000) The phase diagram of the monoolein/water system: metastability and equilibrium aspects. Biomaterials 21: 223-234.
6. Cherezov V, Peddi A, Muthusubramaniam L, Zheng YF, Caffrey M, (2004) A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Acta Cryst D 60: 1795-1807.
7. Caffrey M, Cherezov V, (2009) Crystallizing membrane proteins using lipidic mesophases. Nature Protoc 4: 706-731.
8. Cheng A, Hummel B, Caffrey M, (1998) A simple mechanical mixer for small viscous lipid-containing samples. Chem Phys Lipids 95: 11-21.
9. Enström S, Alfons K, Rasmusson M, Ljusberg-Wahren H, (1998) Solvent-induced sponge (L3) phases in the solvent-monoolein-water system. Prog Coll Pol Sci S 108: 93-98.
10. Wöhri AB, Johansson LC, Wadsten-Hindrichsen P, Wahlgren WY, Fischer G, et al. (2008) Lipidic-sponge phase screen for membrane protein crystallization. Structure 16: 1003-1009.
11. Wadsten P, Wöhri AB, Snijder A, Katona G, Gardiner AT, et al. (2006) Lipidic sponge phase crystallization of membrane proteins. J Mol Biol 364: 44-53.
12. Nollert P, Qiu H, Caffrey M, Rosenbusch JP, Landau EM, (2001) Molecular mechanism for the crystallisation of BR in lipidic cubic phases. FEBS Letters 504: 179-186.
13. Kolbe M, Besir H, Essen LO, Oesterhelt D, (2000) Structure of the light-driven chloride pump Halorhodopsin at 1.8 A resolution. Science 288: 1358-1359.
14. Grabe M, Neu J, Oster G, Nollert P, (2003) Protein interaction and membrane geometry. Biophys J 84: 854-868.
15. Luecke H, Schobert B, Richter HT, Cartailler JP, Lanyi JK, (1999) Structure of Bacteriorhodopsin at 1.55 Å. J Mol Biol 291: 899-911.
16. Michel H, (1982) Characterization and crystal packing of three-dimensional BR crystals. EMBO J 1: 1267-1271.
17. Luecke H, Schobert B, Lanyi JK, Spudich EN, Spudich JL, (2001) Crystal structure of Sensory Rhodopsin II at 2.4 Å: insights into color tuning and transducer interaction. Science 293: 1499-1503.
18. Royant A, Nollert P, Edman K, Neutze R, Landau EM, et al. (2001) X-ray structure of sensory rhodopsin II at 2.1-Å resolution. Proc Natl Acad Sci USA 98: 10131-10136.
19. Gordeliy VI, Labahn J, Moukhametzianov R, Efremov R, Granzin J, et al. (2002) Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex. Nature 419: 484-487.
20. Misquitta Y, Caffrey M, (2003) Detergents destabilize the cubic phase of monoolein: implications for membrane protein crystallization. Biophys J 85: 3084-3096.
21. Misquitta LV, Misquitta Y, Cherezov V, Slattery O, Mohan JM, et al. (2004) Membrane crystallisation in lipidic cubic meso phases with tailored bilayers. Structure 12: 2113-2124.
22. Brumshtein B, Greenblatt HM, Futerman AH, Silman I, Sussman JL, (2008) Control of the rate of evaporation in protein crystallization by the microbatch under oil method. J Appl Cryst 41: 969-971.
23. Chayen NE, (1997) A novel technique to control the rate of vapor diffusion, giving larger protein crystals. J Appl Cryst 30: 198-202.
24. Rassmussen SGF, Choi HJ, Rosenbaum DM, Kobilka TS, Thian FS, et al. (2007) Crystal structure of the human beta2 adrenergic G-protein-coupled receptor. Nature 450: 383-388.
25. Cherezov V, Rosenbaum DM, Hanson MA, Rasmussen SGF, Thian FS, et al. (2007) High resolution crystal structure of an engineered human β2-adrenergic G protein-coupled receptor. Science 318: 1258-1265.
26. Hato M, Yamashita J, Shiono M, (2009) Aqueous phase behavior of lipids with isoprenoid type hydrophobic chains. J Phys Chem B 113: 10196.
27. Misquitta Y, Cherezov V, Havas F, Patterson S, Mohan, et al. (2004) Rational design of lipid for membrane protein crystallization. J Struct Biol 148: 169-175.
28. Liu W, Hanson M, Stevens R, Cherezov V, (2010) LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment. Biophys J 98: 1539-1548.
29. Moiseeva ES, Reshetnyak AB, Borshchevsky VI, Baeken C, Büldt G, et al. (2008) Comparative analysis of the quality of membrane protein Bacteriorhodopsin crystals during crystallization in octylglycoside and octylthioglucoside. J Surface Invest 3: 29-32.
30. Mironova OS, Efremov RG, Person B, Heberle J, Budayak, et al. (2005) Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli. FEBS Lett 579: 3147-3151.
31. Heymann JA, Havelka WA, Oesterhelt D, (1993) Homologous overexpression of a light-driven anion pump in an archaebacterium. Mol Microbiol 7: 623-630.
32. Kors CA, Wallace E, Davies DR, Li L, Laiblea PD, et al. (2009) Effects of impurities on membrane-protein crystallization in different systems. Acta Cryst D 65: 1062-1073.
33. Caffrey M, (2008) On the mechanism of membrane protein crystallization in lipidic mesophases. Cryst Growth Des 8: 4244-4254.