Gap26, a connexin mimetic peptide, inhibits currents carried by connexin43 hemichannels and gap junction channels

Pharmacological Research

Volumn 65, Issue 5, 2012, Pages 546-552

  Desplantez, T ^a   Verma, V ^b,d   Leybaert, L ^c   Evans, W H ^b   Weingart, R ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c GHENT UNIVERSITY   (Belgium)

^d UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Connexin43; Electrophysiology; Gap junction channel; Gap26; Hemichannel

Indexed keywords

CALCIUM; CONNEXIN 43; PEPTIDE GAP26; PEPTIDOMIMETIC AGENT; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM CELL LEVEL; CELL COMMUNICATION; CELL COMPONENT; CELL HEMICHANNEL; CELL MEMBRANE POTENTIAL; CONTROLLED STUDY; ELECTRIC ACTIVITY; ELECTRIC CURRENT; ELECTROPHYSIOLOGY; GAP JUNCTION; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; REACTION TIME;

CONNEXIN 43; CONNEXINS; ELECTROPHYSIOLOGICAL PHENOMENA; GAP JUNCTIONS; HELA CELLS; HUMANS; MEMBRANE POTENTIALS; PEPTIDES; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 84859802065     PISSN: 10436618     EISSN: 10961186     Source Type: Journal    
DOI: 10.1016/j.phrs.2012.02.002     Document Type: Article

References (51)

1. Willecke K, Eiberger J, Degen J, Eckardt D, Romualdi A, Guldenagel M, et al. Structural and functional diversity of connexin genes in the mouse and human genome. Biol Chem 2002;383:725-37. (Pubitemid 34649891)
2. Evans WH, De Vuyst E, Leybaert L. The gap junction cellular internet: connexin hemichannels enter the signalling limelight. Biochem J 2006;397:1-14. (Pubitemid 44032830)
3. Spray DC, Rozental R, Srinivas M. Prospects for rational development of pharmacological gap junction channel blockers. Curr Drug Targets 2002;3:455-64. (Pubitemid 35331902)
4. 2+ signaling through gap junctions in airway cells. Am J Physiol Lung Cell Mol Physiol 2000;279:L623-30.
5. Dhein S. Peptides acting at gap junctions. Peptides 2002;23:1701-9. (Pubitemid 35246290)
6. Herve JC, Dhein S. Peptides targeting gap junctional structures. Curr Pharm Des 2010;16:3056-70.
7. Berman RS, Martin PEM, Evans WH, Griffith TM. Relative contributions of NO and gap junctional communication to endothelium-dependent relaxations of rabbit resistance arteries vary with vessel size. Microvasc Res 2002;63:115-28. (Pubitemid 34971514)
8. Isakson BE, Evans WH, Boitano S. Intercellular Ca2+ signaling in alveolar epithelial cells through gap junctions and by extracellular ATP. Am J Physiol-Lung Cell Mol Physiol 2001;280:L221-8.
9. Anselmi F, Hernandez VH, Crispino G, Seydel A, Ortolano S, Roper SD, et al. ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear. Proc Natl Acad Sci U S A 2008;105:18770-5.
10. Braet K, Vandamme W, Martin PE, Evans WH, Leybaert L. Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26. Cell Calcium 2003;33:37-48. (Pubitemid 36230881)
11. Verma V, Hallett MB, Leybaert L, Martin PE, Evans WH. Perturbing plasma membrane hemichannels attenuates calcium signalling in cardiac cells and HeLa cells expressing connexins. Eur J Cell Biol 2009;88:79-90.
12. De Vuyst E, Decrock E, De Bock M, Yamasaki H, Naus CC, Evans WH, et al. Connexin hemichannels and gap junction channels are differentially influenced by lipopolysaccharide and basic fibroblast growth factor. Mol Biol Cell 2007;18:34-46. (Pubitemid 46066708)
13. Jiang JX, Gu S. Gap junction- and hemichannel-independent actions of connexins. Biochim Biophys Acta 2005;1711:208-14.
14. Saez JC, Retamal MA, Basilio D, Bukauskas FF, Bennett MVL. Connexin-based gap junction hemichannels: gating mechanisms. Biochim Biophys Acta 2005;1711:215-24.
15. Decrock E, De Vuyst E, Vinken M, Van Moorhem M, Vranckx K, Wang N, et al. Connexin 43 hemichannels contribute to the propagation of apoptotic cell death in a rat C6 glioma cell model. Cell Death Differ 2009;16:151-63.
16. Harris AL. Emerging issues of connexin channels: biophysics fills the gap. Q Rev Biophys 2001;34:325-72.
17. Ebihara L, Berthoud VM, Beyer EC. Distinct behavior of connexin56 and connexin46 gap junctional channels can be predicted from the behavior of their hemi-gap-junctional channels. Biophys J 1995;68:1796-803.
18. Contreras JE, Saez JC, Bukauskas FF, Bennett MVL. Gating and regulation of connexion 43 (Cx43) hemichannels. Proc Natl Acad Sci U S A 2003;100:11388-93. (Pubitemid 37205945)
19. De Vries SH, Schwartz EA. Hemi-gap-junction channels in solitary horizontal cells of the catfish retina. J Physiol 1992;445:201-30.
20. Kondo RP, Wang SY, John SA, Weiss JN, Goldhaber JI. Metabolic inhibition activates a non-selective current through connexin hemichannels in isolated ventricular myocytes. J Mol Cell Cardiol 2000;32:1859-72.
21. Li H, Liu TF, Lazrak A, Peracchia C, Goldberg GS, Lampe PD, et al. Properties and regulation of gap junctional hemichannels in the plasma membranes of cultured cells. J Cell Biol 1996;134:1019-30. (Pubitemid 26278225)
22. Valiunas V, Beyer EC, Brink PR. Cardiac gap junction channels show quantitative differences in selectivity. Circ Res 2002;91:104-11. (Pubitemid 34827220)
23. Valiunas V, Weingart R. Electrical properties of gap junction hemichannels identified in transfected HeLa cells. Pflügers Arch 2000;440:366-79. (Pubitemid 30445710)
24. Chaytor AT, Evans WH, Griffith TM. Peptides homologous to extracellular loop motifs of connexin 43 reversibly abolish rhythmic contractile activity in rabbit arteries. J Physiol 1997;503(Pt 1):99-110. (Pubitemid 27390023)
25. Bader P, Weingart R. Conductive and kinetic properties of connexin45 hemichannels expressed in transfected HeLa cells. J Membr Biol 2004;199:143-54. (Pubitemid 38982204)
26. Bader P, Weingart R. Pitfalls when examining gap junction hemichannels: interference from volume-regulated anion channels. Pflügers Arch 2006;452:396-406. (Pubitemid 43962049)
27. Desplantez T, Halliday D, Dupont E, Weingart R. Cardiac connexins Cx43 and Cx45: formation of diverse gap junction channels with diverse electrical properties. Pflügers Arch 2004;448:363-75. (Pubitemid 39006000)
28. Spray DC, Ye ZC, Ransom BR. Functional connexin hemichannels: a critical appraisal. Glia 2006;54:758-73. (Pubitemid 44629638)
29. Hawat G, Benderdour M, Rousseau G, Baroudi G. Connexin43 mimetic peptide Gap26 confers protection to intact heart against myocardial ischemia injury. Pflügers Arch 2010;460:583-92.
30. Valiunas V, Weingart R, Brink PR. Formation of heterotypic gap junction channels by connexins 40 and 43. Circ Res 2000;86(February (2)):E42-9.
31. Desplantez T, Marics I, Jarry-Guichard T, Veteikis R, Briand JP, Weingart R, et al. Characterization of zebrafish Cx43.4 connexin and its channels. Exp Physiol 2003;88(November (6)):681-90.
32. Bukauskas FF, Kempf C, Weingart R. Cytoplasmic bridges and gap junctions in an insect cell line (Aedes albopictus). J Exp Physiol 1992;77:903-11.
33. Berthoud VM, Minogue PJ, Laing JG, Beyer EC. Pathways for degradation of connexins and gap junctions. Cardiovasc Res 2004;62:256-67. (Pubitemid 38496539)
34. Tang Q, Dowd TL, Verselis VK, Bargiello TA. Conformational changes in a pore-forming region underlie voltage-dependent loop gating of an unapposed connexin hemichannel. J Gen Physiol 2009;133:555-70.
35. Kwak BR, Hermans MM, De Jonge HR, Lohmann SM, Jongsma HJ, Chanson M. Differential regulation of distinct types of gap junction channels by similar phosphorylating conditions. Mol Biol Cell 1995;6(December (12)):1707-19.
36. Moreno AP, Sáez JC, Fishman GI, Spray DC. Human connexin43 gap junction channels. Regulation of unitary conductances by phosphorylation. Circ Res 1994;74(June (6)):1050-7.
37. Pollok S, Pfeiffer AC, Lobmann R, Wright CS, Moll I, Martin PE, et al. Connexin 43 mimetic peptide Gap27 reveals potential differences in the role of Cx43 in wound repair between diabetic and non-diabetic cells. J Cell Mol Med 2011;15(April (4)):861-73.
38. Foote CI, Zhou L, Zhu X, Nicholson BJ. The pattern of disulfide linkages in the extracellular loop regions of connexin 32 suggests a model for the docking interface of gap junctions. J Cell Biol 1998;140(March (5)):1187-97.
39. Martin PE, Evans WH. Incorporation of connexins into plasma membranes and gap junctions. Cardiovasc Res 2004;62(May (2)):378-87 (Review).
40. Thomas T, Jordan K, Simek J, Shao Q, Jedeszko C, Walton P, et al. Mechanisms of Cx43 and Cx26 transport to the plasma membrane and gap junction regeneration. J Cell Sci 2005;118(October (Pt 19)):4451-62.
41. Rhett JM, Jourdan J, Gourdie RG. Connexin 43 connexon to gap junction transition is regulated by zonula occludens-1. Mol Biol Cell 2011;22(May (9)):1516-28.
42. Verselis VK, Trelles MP, Rubinos C, Bargiello TA, Srinivas M. Loop gating of connexin hemichannels involves movement of pore-lining residues in the first extracellular loop domain. J Biol Chem 2009;284:4484-93.
43. Sosinsky GE, Nicholson BJ. Structural organization of gap junction channels. Biochim Biophys Acta 2005;1771:99-125.
44. Bukauskas FF, Verselis VK. Gap junction channel gating. Biochim Biophys Acta 2004;1662:42-60.
45. Peracchia C. Chemical gating of gap junction channels. Roles of calcium, pH and calmodulin. Biochim Biophys Acta 2004;1662:61-80.
46. Boitano S, Dirksen ER, Evans WH. Sequence specific antibodies to connexins block intercellular calcium signaling through gap junctions. Cell Calcium 1998;23:1-9. (Pubitemid 28159592)
47. Paemeleire K, Martin PE, Coleman SL, Fogarty KE, Carrington WA, Leybaert L, et al. Intercellular calcium waves in HeLa cells expressing GFP-labeled connexin 43, 32, or 26. Mol Biol Cell 2000;11:1815-27. (Pubitemid 30307915)
48. Toyofuku T, Yabuki M, Otsu K, Kuzuya T, Hori M, Tada M. Intercellular calcium signaling via gap junction in connexin-43-transfected cells. J Biol Chem 1998;273:1519-28. (Pubitemid 28133675)
49. Parthasarathi K, Ichimura H, Monma E, Lindert J, Quadri S, Issekutz A, et al. Connexin 43 mediates spread of Ca2+-dependent proinflammatory responses in lung capillaries. J Clin Invest 2006;116(August (8)):2193-200.
50. De Vuyst E, Wang N, Decrock E, De Bock M, Vinken M, Van Moorhem M, et al. Ca(2+) regulation of connexin 43 hemichannels in C6 glioma and glial cells. Cell Calcium 2009;46(September (3)):176-87.
51. Bargiotas P, Monyer H, Schwaninger M. Hemichannels in cerebral ischemia. Curr Mol Med 2009;9:186-94.