Intercellular calcium waves in HeLa cells expressing GFP-labeled connexin 43, 32, or 26

Molecular Biology of the Cell

Volumn 11, Issue 5, 2000, Pages 1815-1827

  Paemeleire, Koen ^b   Martin, Patricia E M ^c   Coleman, Sharon L ^c   Fogarty, Kevin E ^a   Carrington, Walter A ^a   Leybaert, Luc ^b   Tuft, Richard A ^a   Evans, W Howard ^c   Sanderson, Michael J ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b GHENT UNIVERSITY   (Belgium)

^c CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

APYRASE; CALCIUM; CONNEXIN 26; CONNEXIN 32; CONNEXIN 43; FLUORESCENT DYE; FURA 2; GREEN FLUORESCENT PROTEIN;

ARTICLE; CALCIUM TRANSPORT; CELL COMMUNICATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GAP JUNCTION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; MICROSCOPE IMAGE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0034026936     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.11.5.1815     Document Type: Article

References (58)

1. Boitano, S., Dirksen, E.R., and Evans W.H. (1998). Sequence-specific antibodies to connexins block intercellular calcium signaling through gap junctions. Cell Calcium 23, 1-9.
2. Boitano, S., Dirksen, E.R., and Sanderson, M.J. (1992). Intercellular propagation of calcium waves mediated by inositol trisphosphate. Science 258, 292-295.
3. 2+ signals underlying waves and graded responses in HeLa cells. Curr. Biol. 6, 855-865.
4. 2+ mobilization from the intracellular stores of single histamine-stimulated HeLa cells. J. Physiol. (Lond.) 450, 162-178.
5. Cao, F., Eckert, R., Elfgang, C., Nitsche, J.M., Snyder, S.A., H-ulser, D.F., Willecke, K., and Nicholson, B.J. (1998). A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes. J. Cell Sci. 111, 31-43.
6. Carrington, W.A., Lynch, R.M., Moore, E.D., Isenberg, G., Fogarty, K.E., and Fay, F.S. (1995). Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. Science 268, 1483-1487.
7. Charles, A. (1998). Intercellular calcium waves in glia. Glia 24, 39-49.
8. Charles, A.C., Merrill, J.E., Dirksen, E.R., and Sanderson, M.J. (1991). Intercellular signaling in glial cells: calcium waves and oscillations in response to mechanical stimulation and glutamate. Neuron 6, 983-992.
9. Charles, A.C., Naus, C.C., Zhu, D., Kidder, G.M., Dirksen, E.R., and Sanderson, M.J. (1992). Intercellular calcium signaling via gap junctions in glioma cells. J. Cell Biol. 118, 195-201.
10. Churchill, G.C., Atkinson, M.M., and Louis, C.F. (1996). Mechanical stimulation initiates celt-to-cell calcium signaling in ovine lens epithelial cells. J. Cell Sci. 109, 355-365.
11. 2+ waves in astrocytes. Cell Calcium 12, 185-204.
12. Cotrina, M.L., Lin, J.H., Alves-Rodrigues, A., Liu, S., Li, J., Azmi-Ghadimi, H., Kang, J., Naus, C.C., and Nedergaard, M. (1998). Connexins regulate calcium signaling by controlling ATP release. Proc. Natl. Acad. Sci. USA 95, 15735-15740.
13. Diez, J.A., Ahmad, S., and Evans, W.H. (1999). Assembly of heteromeric connexons in guinea-pig liver en route to the golgi apparatus, plasma membrane and gap junctions. Eur. J. Biochem. 262, 142-148.
14. Enkvist, M.O., and McCarthy, K.D. (1992). Activation of protein kinase C blocks astroglial gap junction communication and inhibits the spread of calcium waves. J. Neurochem. 59, 519-526.
15. Finkbeiner, S. (1992). Calcium waves in astrocytes: filling in the gaps. Neuron 8, 1101-1108.
16. Frame, M.K., and de Feijter, A.W. (1997). Propagation of mechanically induced intercellular calcium waves via gap junctions and ATP receptors in rat liver epithelial cells. Exp. Cell Res. 230, 197-207.
17. Furuya, K., Enomoto, K., Maeno, T., and Yamagishi, S. (1993). Mechanically induced calcium signal in mammary epithelial cells. Jpn. J. Physiol. 43(Suppl 1), S105-108.
18. George, C.H., Kendall, J.M., Campbell, A.K., and Evans, W.H. (1998a). Connexin-aequorin chimerae report cytoplasmic calcium environments along trafficking pathways leading to gap junction biogenesis in living COS-7 cells. J. Biol. Chem. 273, 29822-29829.
19. George, C.H., Martin, P.E., and Evans, W.H. (1998b). Rapid determination of gap junction formation using HeLa cells microinjected with cDNAs encoding wild-type and chimeric connexins. Biochem. Biophys. Res. Commun. 247, 785-789.
20. 2+, in co-perfused smooth muscle cells. FEBS Lett. 240, 59-64.
21. Graeber, S.H., and Hulser, D.F. (1998). Connexin transfection induces invasive properties in HeLa cells. Exp. Cell Res. 243, 142-149.
22. Grandolfo, M., Calabrese, A., and D'Andrea, P. (1998). Mechanism of mechanically induced intercellular calcium waves in rabbit articular chondrocytes and in HIG-82 synovial cells. J. Bone Miner. Res. 13, 443-453.
23. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3450.
24. Guthrie, P.B., Knappenberger, J., Segal, M., Bennett, M.V.L., Charles, A.C., and Kater, S.B. (1999). ATP released from astrocytes mediates glial calcium waves. J. Neurosci. 19, 520-528.
25. Hansen, M., Boitano, S., Dirksen, E.R., and Sanderson, M.J. (1993). Intercellular calcium signaling induced by extracellular adenosine 5′-triphosphate and mechanical stimulation in airway epithelial cells. J. Cell Sci. 106, 995-1004.
26. Hassinger, T.D., Guthrie, P.B., Atkinson, P.B., Bennett, M.V., and Kater, S.B. (1996). An extracellular signaling component in propagation of astrocyric calcium waves. Proc. Natl. Acad. Sci. USA 93, 13268-13273.
27. 2+ signaling in retinal pigment epithelial cells during mechanical stimulation. FASEB J. 13, S63-68.
28. Jordan, K., Solan, J.L., Dominguez, M., Sia, M., Hand, A., Lampe, P.D., and Laird, D.W. (1999). Trafficking, assembly, and function of a connexin43-green fluorescent protein chimera in live mammalian cells. Mol. Biol. Cell 10, 2033-2050.
29. Kasai, H. (1995). Pancreatic calcium waves and secretion. Ciba Found. Symp. 188, 104-116.
30. 2+ signals in mouse pancreatic acinar cells. J. Physiol. (Lond.) 520, 187-201.
31. Koch, G.L. (1990). The endoplasmic reticulum and calcium storage. BioEssays 12, 527-531.
32. Kumar, N.M., and Gilula, N.B. (1992). Molecular biology and genetics of gap junction channels. Semin. Cell Biol. 3, 3-16.
33. 2+. Biophys. J. 77, 629-638.
34. Leybaert, L., Paemeleire, K., Strahonja, A., and Sanderson, M.J. (1998a). Inositol-trisphosphate-dependent intercellular calcium signaling in and between astrocytes and endothelial cells. Glia 24, 398-407.
35. Leybaert, L., Sneyd, J., and Sanderson, M.J. (1998b). A simple method for high temporal resolution calcium imaging with dual excitation dyes. Biophys. J. 75, 2025-2029.
36. Martin, P.E., George, C.H., Castro, C., Kendall, J.M., Capel, J., Campbell, A.K., Revilla, A., Barrio, L.C., and Evans, W.H. (1998). Assembly of chimeric connexin-aequorin proteins into functional gap junction channels. Reporting intracellular and plasma membrane calcium environments. J. Biol. Chem. 273, 1719-1726.
37. Osipchuk, Y., and Cahalan, M. (1992). Cell-to-cell spread of calcium signals mediated by ATP receptors in mast cells. Nature 359, 241-244.
38. Perez-Terzic, C., Stehno-Bittel, L., and Clapham, D.E. (1997). Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3. Cell Calcium 21, 275-282.
39. 2+ store, with functional properties distinct from those of the endoplasmic reticulum. EMBO J. 17, 5298-5308.
40. 2+ responses. Science 280, 1763-1766.
41. 2+ waves in the intact liver. J. Biol. Chem. 270, 8102-8107.
42. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
43. Sanderson, M.J. (1995). Intercellular calcium waves mediated by inositol trisphosphate. Ciba Found. Symp. 188, 175-189.
44. Sanderson, M.J., Charles, A.C., Boitano, S., and Dirksen, E.R. (1994). Mechanisms and function of intercellular calcium signaling. Mol. Cell. Endocrinol. 98, 173-187.
45. 2+ in epithelial cells. Cell Regul. 1, 585-596.
46. Schroder, H.C., Ugarkovic, D., Merz, H., Kuchino, Y., Okamoto, T., and Muller, W.E. (1990). Protection of HeLa-T4+ cells against human immunodeficiency virus (HIV) infection after stable transfection with HIV LTR-2′,5′-oligoadenylate synthetase hybrid gene. FASEB J. 4, 3124-3130.
47. Simon, A.M. (1999). Gap junctions: more roles and new structural data. Trends Cell Biol. 9, 169-170.
48. Simon, A.M., and Goodenough, D.A. (1998). Diverse functions of vertebrate gap junctions. Trends Cell Biol. 8, 477-483.
49. 2+ in HeLa cells by the interaction with a 5′-nucleotide receptor. Eur. J. Pharmacol. 247, 223-226.
50. 2+ oscillations in exocrine cells evoked by agonists and inositol trisphosphate. Cell 74, 661-668.
51. Touitou, I., Mathieu, M., and Rochefort, H. (1990). Stable transfection of the estrogen receptor cDNA into HeLa cells induces estrogen responsiveness of endogenous cathepsin D gene but not of cell growth. Biochem. Biophys. Res. Commun. 169, 109-115.
52. Toyofuku, T., Yabuki, M., Otsu, K., Kuzuya, T., Hori, M., and Tada, M. (1998). Intercellular calcium signaling via gap junction in connexin-43-transfected cells. J. Biol Chem. 273, 1519-1528 (erratum 273, 22856).
53. Venance, L., Stella, N., Glowinski, J., and Giaume, C. (1997). Mechanism involved in initiation and propagation of receptor-induced intercellular calcium signaling in cultured rat astrocytes. J. Neurosci. 17, 1981-1992.
54. Wilkinson, G.W., and Akrigg, A. (1992). Constitutive and enhanced expression from the CMV major IE promoter in a defective adenovirus vector. Nucleic Acids Res. 20, 2233-2239.
55. 2+ evoked by inositol trisphosphate in Xenopus oocytes. J. Physiol. (Lond.) 482:533-553.
56. Yeager, M., Unger, V.M., and Falk, M.M. (1998). Synthesis, assembly and structure of gap junction intercellular channels. Curr. Opin. Struct. Biol. 8:517-524.
57. 2+ sparks and spontaneous transient outward currents in single smooth muscle cells. J. Gen. Physiol. 113, 215-228.
58. 2+ waves in the blowfly salivary gland. EMBO J. 18, 3222-3231.