An efficient method to limit microglia-dependent effects in astroglial cultures

Journal of Neuroscience Methods

Volumn 207, Issue 1, 2012, Pages 59-71

  Losciuto, Sophie ^a   Dorban, Gauthier ^a   Gabel, Sébastien ^a   Gustin, Audrey ^a   Hoenen, Claire ^a   Grandbarbe, Luc ^a   Heuschling, Paul ^a   Heurtaux, Tony ^a  

^a UNIVERSITY OF LUXEMBOURG   (Luxembourg)

Author keywords

Astrocytes; Beta amyloid; Brain; Immune cells; Inflammation

Indexed keywords

AMYLOID BETA PROTEIN; GAMMA INTERFERON; LIPOPOLYSACCHARIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ASTROCYTE; CELL ACTIVATION; CELL CULTURE; CELL SELECTION; CELL VIABILITY; CONTROLLED STUDY; INFLAMMATION; MICROGLIA; MOUSE; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ANIMALS; ASTROCYTES; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION PROFILING; INFLAMMATION; MICE; MICE, INBRED C57BL; MICROGLIA; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84859708632     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2012.03.010     Document Type: Article

References (50)

1. Amor S., Puentes F., Baker D., van der Valk P. Inflammation in neurodegenerative diseases. Immunology 2010, 129:154-169.
2. Beutner C., Roy K., Linnartz B., Napoli I., Neumann H. Generation of microglial cells from mouse embryonic stem cells. Nat Protoc 2010, 5:1481-1494.
3. Block M.L., Zecca L., Hong J.S. Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat Rev Neurosci 2007, 8:57-69. [Review].
4. Booher J., Sensenbrenner M. Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiology 1972, 2:97-105.
5. Bramanti V., Tomassoni D., Avitabile M., Amenta F., Avola R. Biomarkers of glial cell proliferation and differentiation in culture. Front Biosci (Schol Ed) 2010, 2:558-570. [Review].
6. Buchanan M.M., Hutchinson M., Watkins L.R., Yin H. Toll-like receptor 4 in CNS pathologies. J Neurochem 2010, 114:13-27. [Review].
7. Buffo A., Rite I., Tripathi P., Lepier A., Colak D., Horn A.P., et al. Origin and progeny of reactive gliosis: a source of multipotent cells in the injured brain. Proc Natl Acad Sci U S A 2008, 105:3581-3586.
8. Carmody R.J., Chen Y.H. Nuclear factor-kappaB: activation and regulation during toll-like receptor signaling. Cell Mol Immunol 2007, 4:31-41.
9. Cho S.H., Chen C.H., Tsai F.S., Godin J.M., Lo Y.H. Human mammalian cell sorting using a highly integrated micro-fabricated fluorescence-activated cell sorter (microFACS). Lab Chip 2010, 10:1567-1573.
10. Crocker S.J., Frausto R.F., Whitton J.L., Milner R. A novel method to establish microglia-free astrocyte cultures: comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia. Glia 2008, 56:1187-1198.
11. Dainiak M.B., Kumar A., Galaev I.Y., Mattiasson B. Methods in cell separations. Adv Biochem Eng Biotechnol 2007, 106:1-18.
12. Dobrenis K. Microglia in cell culture and in transplantation therapy for central nervous system disease. Methods 1998, 16:320-344. [Review].
13. Ebenezer P.J., Weidner A.M., Levine Iii H., Markesbery W.R., Murphy M.P., Zhang L., et al. Neuron specific toxicity of oligomeric amyloid-β: role for JUN-kinase and oxidative stress. J Alzheimers Dis 2010, 22:839-848.
14. Ekdahl C.T., Kokaia Z., Lindvall O. Brain inflammation and adult neurogenesis: the dual role of microglia. Neuroscience 2009, 158:1021-1029. [Review].
15. Farfara D., Lifshitz V., Frenkel D. Neuroprotective and neurotoxic properties of glial cells in the pathogenesis of Alzheimer's disease. J Cell Mol Med 2008, 12:762-780.
16. Floden A.M., Combs C.K. Microglia repetitively isolated from in vitro mixed glial cultures retain their initial phenotype. J Neurosci Methods 2007, 164:218-224.
17. Fuller S., Steele M., Münch G. Activated astroglia during chronic inflammation in Alzheimer's disease - do they neglect their neurosupportive roles. Mutat Res 2010, 690:40-49.
18. Fuss I.J., Kanof M.E., Smith P.D., Zola H. Isolation of whole mononuclear cells from peripheral blood and spinal cord. Curr Protocol Immunol 2009, [chapter 7: unit 7.1].
19. Gough D.J., Levy D.E., Johnstone R.W., Clarke C.J. IFNgamma signaling-does it mean JAK-STAT. Cytokine Growth Factor Rev 2008, 19:383-394.
20. Graham J.M. Isolation of lysosomes from tissues and cells by differential and density gradient centrifugation. Curr Protoc Cell Biol 2001, [chapter 3: unit 3.6].
21. Grandbarbe L., Bouissac J., Rand M., Hrabé de Angelis M., Artavanis-Tsakonas S., Mohier E. Delta-notch signaling controls the generation of neurons/glia from neural stem cells in a stepwise process. Development 2003, 130:1391-1402.
22. Hamby M.E., Sofroniew M.V. Reactive astrocytes as therapeutic targets for CNS disorders. Neurotherapeutics 2010, 7:494-506.
23. Hamby M.E., Uliasz T.F., Hewett S.J., Hewett J.A. Characterization of an improved procedure for the removal of microglia from confluent monolayers of primary astrocytes. J Neurosci Methods 2006, 150:128-137.
24. Hamby M.E., Hewett J.A., Hewett S.J. TGF-beta1 potentiates astrocytic nitric oxide production by expanding the population of astrocytes that express NOS-2. Glia 2006, 54:566-577.
25. Hanisch U.K., Kettenmann H. Microglia: active sensor and versatile effector cells in the normal and pathologic brain. Nat Neurosci 2007, 10:1387-1394.
26. Henze C., Hartmann A., Lescot T., Hirsch E.C., Michel P.P. Proliferation of microglial cells induced by 1-methyl-4-phenylpyridinium in mesencephalic cultures results from an astrocyte-dependent mechanism: role of granulocyte macrophage colony-stimulating factor. J Neurochem 2005, 95:1069-1077.
27. Heurtaux T., Michelucci A., Losciuto S., Gallotti C., Felten P., Dorban G., et al. Microglial activation depends on beta-amyloid conformation: role of the formylpeptide receptor 2. J Neurochem 2010, 114:576-586.
28. Hewett J.A., Hewett S.J., Winkler S., Pfeiffer S.E. Inducible nitric oxide synthase expression in cultures enriched for mature oligodendrocytes is due to microglia. J Neurosci Res 1999, 56:189-198.
29. Jack C.S., Arbour N., Manusow J., Montgrain V., Blain M., McCrea E., et al. TLR signaling tailors innate immune responses in human microglia and astrocytes. J Immunol 2005, 175:4320-4330.
30. Jana M., Jana A., Pal U., Pahan K. A simplified method for isolating highly purified neurons, oligodendrocytes, astrocytes, and microglia from the same human fetal brain tissue. Neurochem Res 2007, 32:2015-2022.
31. Kim Y.S., Joh T.H. Microglia, major player in the brain inflammation: their roles in the pathogenesis of Parkinson's disease. Exp Mol Med 2006, 38:333-347. [Review].
32. Lara O.R., Chalmers J.J. Cell enrichment and immunochemical staining. Methods Mol Biol 2005, 295:301-310.
33. Lehnardt S., Lachance C., Patrizi S., Lefebvre S., Follett P.L., Jensen F.E., et al. The toll-like receptor TLR4 is necessary for lipopolysaccharide-induced oligodendrocyte injury in the CNS. J Neurosci 2002, 22:2478-2486.
34. Liu W., Tang Y., Feng J. Cross talk between activation of microglia and astrocytes in pathological conditions in the central nervous system. Life Sci 2011, 89:141-146. [Review].
35. Lull M.E., Block M.L. Microglial activation and chronic neurodegeneration. Neurotherapeutics 2010, 7:354-365.
36. Marek R., Caruso M., Rostami A., Grinspan J.B. Das Sarma J. Magnetic cell sorting: a fast and effective method of concurrent isolation of high purity viable astrocytes and microglia from neonatal mouse brain tissue. J Neurosci Methods 2008, 175:108-118.
37. Michelucci A., Heurtaux T., Grandbarbe L., Morga E., Heuschling P. Characterization of the microglial phenotype under specific pro-inflammatory and anti-inflammatory conditions: effects of oligomeric and fibrillar amyloid-beta. J Neuroimmunol 2009, 210:3-12.
38. Middeldorp J., Hol E.M. GFAP in health and disease. Prog Neurobiol 2011, 93:421-443.
39. Mishra B.B., Mishra P.K., Teale J.M. Expression and distribution of Toll-like receptors in the brain during murine neurocysticercosis. J Neuroimmunol 2006, 181:46-56.
40. Peri F., Piazza M., Calabrese V., Damore G., Cighetti R. Exploring the LPS/TLR4 signal pathway with small molecules. Biochem Soc Trans 2010, 38:1390-1395.
41. Perry V.H., Nicoll J.A., Holmes C. Microglia in neurodegenerative disease. Nat Rev Neurol 2010, 6:193-201.
42. Röhl C., Lucius R., Sievers J. The effect of activated microglia on astrogliosis parameters in astrocyte cultures. Brain Res 2007, 1129:43-52.
43. Saijo K., Winner B., Carson C.T., Collier J.G., Boyer L., Rosenfeld M.G., et al. A Nurr1/CoREST pathway in microglia and astrocytes protects dopaminergic neurons from inflammation-induced death. Cell 2009, 137:47-59.
44. Saura J. Microglial cells in astroglial cultures: a cautionary note. J Neuroinflamm 2007, 4:26. [Review].
45. Sofroniew M.V. Molecular dissection of reactive astrogliosis and glial scar formation. Trends Neurosci 2009, 32:638-647. [Review].
46. Sofroniew M.V., Vinters H.V. Astrocytes: biology and pathology. Acta Neuropathol 2010, 119:7-35.
47. Solenov E., Watanabe H., Manley G.T., Verkman A.S. Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method. Am J Physiol Cell Physiol 2004, 286:426-432.
48. Vallejo R., Tilley D.M., Vogel L., Benyamin R. The role of glia and the immune system in the development and maintenance of neuropathic pain. Pain Pract 2010, 10:167-184. [Review].
49. Zaheer A., Mathur S.N., Lim R. Overexpression of glia maturation factor in astrocytes leads to immune activation of microglia through secretion of granulocyte-macrophage-colony stimulating factor. Biochem Biophys Res Commun 2002, 294:238-244.
50. Zhang D., Hu X., Qian L., O'Callaghan J.P., Hong J.S. Astrogliosis in CNS pathologies: is there a role for microglia?. Mol Neurobiol 2010, 41:232-241. [Review].