Magnetic cell sorting: A fast and effective method of concurrent isolation of high purity viable astrocytes and microglia from neonatal mouse brain tissue

Journal of Neuroscience Methods

Volumn 175, Issue 1, 2008, Pages 108-118

  Marek, Ryan ^a   Caruso, Michael ^a   Rostami, Abdolmohamad ^a   Grinspan, Judith B ^b   Sarma, Jayasri Das ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

^b CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

Author keywords

Astrocytes; Central nervous system; Complement mediated cytolysis; Glial cell isolation; Magnetic beads; Microglia; Neuroinflammation; Oligodendrocytes

Indexed keywords

GELATINASE A; GELATINASE B; INTERLEUKIN 10; INTERLEUKIN 12P40; INTERLEUKIN 13; INTERLEUKIN 17; INTERLEUKIN 18; INTERLEUKIN 1ALPHA; INTERLEUKIN 1BETA; INTERLEUKIN 2; INTERLEUKIN 23; INTERLEUKIN 27; INTERLEUKIN 4; INTERLEUKIN 5; INTERLEUKIN 6; MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA; MACROPHAGE INFLAMMATORY PROTEIN 1BETA; MACROPHAGE INFLAMMATORY PROTEIN 2; MONOCYTE CHEMOTACTIC PROTEIN 1; RANTES; STROMELYSIN; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ANIMAL TISSUE; ANTIGEN ANTIBODY COMPLEX; ARTICLE; ASTROCYTE; BRAIN TISSUE; CELL FUNCTION; CELL ISOLATION; CELL POPULATION; CELL SELECTION; CELL VIABILITY; CONTROLLED STUDY; CYTOLYSIS; GLIA CELL; MAGNETIC FIELD; MICROGLIA; MOUSE; NEWBORN; NONHUMAN; OLIGODENDROGLIA; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; ANIMALS, NEWBORN; ANTIGENS, CD11B; ASTROCYTES; BRAIN; CELL COUNT; CELLS, CULTURED; CYTOKINES; FLOW CYTOMETRY; GANGLIOSIDES; GLIAL FIBRILLARY ACIDIC PROTEIN; IMMUNOMAGNETIC SEPARATION; METALLOPROTEASES; MICE; MICROGLIA;

EID: 52049087817     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2008.08.016     Document Type: Article

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