Isolation of whole mononuclear Cells from peripheral blood and cord blood

Current Protocols in Immunology

Volumn , Issue SUPPL. 85, 2009, Pages

  Fuss, Ivan J ^a   Kanof, Marjorie E ^b   Smith, Phillip D ^c   Zola, Heddy ^d  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b US General Accounting Office   (United States)

^c NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH   (United States)

^d CHILD HEALTH RESEARCH INSTITUTE   (Australia)

Author keywords

Density gradient; Ficoll Hypaque; Mononuclear cell; Peripheral blood

Indexed keywords

DIATRIZOATE; FICOLL; LEUCINE METHYL ESTER;

CELL ADHESION; CELL DENSITY; CELL FRACTIONATION; CELL ISOLATION; CELL SEPARATION; CELL SIZE; CELL SUSPENSION; DENSITY GRADIENT CENTRIFUGATION; HUMAN; MACROPHAGE; MONONUCLEAR CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; REVIEW; SEPARATION TECHNIQUE; UMBILICAL CORD BLOOD;

CELL SEPARATION; CENTRIFUGATION, DENSITY GRADIENT; ERYTHROBLASTS; FETAL BLOOD; FICOLL; HUMANS; IMMUNOLOGIC TECHNIQUES; INFANT, NEWBORN; LEUCINE; LEUKOCYTES, MONONUCLEAR; MACROPHAGES; METHYL ETHERS;

EID: 64249117460     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/0471142735.im0701s85     Document Type: Review

References (12)

1. Boyum, A. 1968. Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Invest. Suppl. 21:77-89.
2. Ferrante, A. and Thong, Y. 1980. Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method. J. Immunol. Methods 36:109.
3. Kaplan, J., Nolan, D., and Ree, A. 1982. Altered lymphocyte markers and blastogenic response associated with 24 hour delay in processing of blood samples. J. Immunol. Methods 50:187-191.
4. Niku, S., Hoon, D., Cochran, A., and Morton, D. 1987. Isolation of lymphocytes from clotted blood. J. Immunol. Methods. 105:9-14.
5. Nisperos, B. 1990. Density gradient isolation of peripheral blood lymphocytes. In ASHI Laboratory manual, 2nd ed. (A.A. Zachary and G.A. Teresi, eds.) pp. 23-27. American Society for Histocompatibility and Immunogenics, Lenexa, Kans.
6. Ridings, J., Weedon, H., Ioannu, C., Flego, L., Macadle, P., and Zola, H. 1996. Purification of cord blood lymphocytes. J. Immunol. Methods. 195:43-48.
7. Skoog, W. and Beck, W. 1956. Studies of the fibrinogen, dextran and phytohaemagglutinin methods of isolating leukocytes. Blood 11:436.
8. Tamul,K., Schmitz, J., Kane, K., and Folds, J. 1995. Comparison of the effects of Ficoll-Hypaque separation and whole blood lysis on results of immunophenotypic analysis of blood and bone marrow samples from patients with hematologic malignancies. Clin. Diag. Lab. Immunol. 2:337-342.
9. Thiele, D., Kurosaka, M., and Lipsky, P. 1983. Phenotype of the accessory cell necessary for mitogen-stimulated T and B cell responses in human peripheral blood: Delineation by its sensitivity to the lysosomotropic agent, L-leucine methyl ester. J. Immunol. 131:2282-2290.
10. Thong, Y., Currell, J., and Rodwell, R. 1983. The rapid one-step gradient centrifugation procedure for simultaneous isolation of granulocytic and mononuclear leukocytes from human blood: Biological, physical and chemical bases. Med. Hypotheses 12:103-111.
11. Tse, H. and Dutton, R. 1976. Separation of helper and suppressor T lymphocytes on a Ficoll velocity sedimentation gradient. J. of Exp. Med. 143:1199-1210.
12. Vissers, M., Jester, S., and Fantone, J. 1988. Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll- Hypaque and Sepracell-MN. J. Immunol. Methods 13:203-207.