Characterization of an improved procedure for the removal of microglia from confluent monolayers of primary astrocytes

Journal of Neuroscience Methods

Volumn 150, Issue 1, 2006, Pages 128-137

  Hamby, Mary E ^a   Uliasz, Tracy F ^a   Hewett, Sandra J ^a   Hewett, James A ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

Astrocytes; Cell culture; Cyclooxygenase 2 (COX 2); Inducible nitric oxide synthase (iNOS; NOS 2); L Leucine methyl ester; Lipopolysaccharide (LPS); Microglia; Nitric oxide (NO); Reactive astrocytosis

Indexed keywords

CYCLOOXYGENASE 2; GAMMA INTERFERON; INDUCIBLE NITRIC OXIDE SYNTHASE; LEUCINE METHYL ESTER;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ASTROCYTE; CELL CULTURE; CELL ISOLATION; CONTROLLED STUDY; ENZYME PURIFICATION; GLIA CELL; MICROGLIA; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

ANIMALS; ANTIMETABOLITES, ANTINEOPLASTIC; ASTROCYTES; CELL SEPARATION; CELLS, CULTURED; CYCLOOXYGENASE 2; CYTARABINE; LEUCINE; MICE; MICE, INBRED STRAINS; MICROGLIA; MITOSIS; NITRIC OXIDE SYNTHASE TYPE II;

EID: 28844446890     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2005.06.016     Document Type: Article

References (44)

1. A. Araque, G. Carmignoto, and P.G. Haydon Dynamic signaling between astrocytes and neurons Annu Rev Physiol 63 2001 795 813
2. K.M. Boje, and P.K. Arora Microglial-produced nitric oxide and reactive nitrogen oxides mediate neuronal cell death Brain Res 587 1992 250 256
3. C.C. Bowman, A. Rasley, S.L. Tranguch, and I. Marriott Cultured astrocytes express toll-like receptors for bacterial products Glia 43 2003 281 291
4. D.R. Brown, B. Schmidt, and H.A. Kretzschmar A neurotoxic prion protein fragment enhances proliferation of microglia but not astrocytes in culture Glia 18 1996 59 67
5. R. Ciccarelli, P. Di Iorio, I. D'Alimonte, P. Giuliani, T. Florio, and F. Caciagli Cultured astrocyte proliferation induced by extracellular guanosine involves endogenous adenosine and is raised by the co-presence of microglia Glia 29 2000 202 211
6. F. Doetsch The glial identity of neural stem cells Nat Neurosci 6 2003 1127 1134
7. Y. Dong, and E.N. Benveniste Immune function of astrocytes Glia 36 2001 180 190
8. M. Eddleston, and L. Mucke Molecular profile of reactive astrocytes - implications for their role in neurologic disease Neuroscience 54 1993 15 36
9. M.R. Ehlers CR3: a general purpose adhesion-recognition receptor essential for innate immunity Microbes Infect 2 2000 289 294
10. S. Fedoroff, W.A. McAuley, J.D. Houle, and R.M. Devon Astrocyte cell lineage. V. Similarity of astrocytes that form in the presence of dBcAMP in cultures to reactive astrocytes in vivo J Neurosci Res 12 1984 14 27
11. R.D. Fields, and B. Stevens-Graham New insights into neuron-glia communication Science 298 2002 556 562
12. M.R. Freeman, S.L. Beckmann, and N. Sueoka Regulation of the S100 protein and GFAP genes is mediated by two common mechanisms in RT4 neuro-glial cell lines Exp Cell Res 182 1989 370 383
13. E. Galea, D.L. Feinstein, and D.J. Reis Induction of calcium-independent nitric oxide synthase activity in primary rat glial cultures Proc Natl Acad Sci USA 89 1992 10945 10949
14. D. Giulian, and T.J. Baker Characterization of ameboid microglia isolated from developing mammalian brain J Neurosci 6 1986 2163 2178
15. D. Giulian, and J.E. Ingeman Colony-stimulating factors as promoters of ameboid microglia J Neurosci 8 1988 4707 4717
16. D. Giulian, K. Vaca, and M. Corpuz Brain glia release factors with opposing actions upon neuronal survival J Neurosci 13 1993 29 37
17. L.C. Green, D.A. Wagner, J. Glogowski, P.L. Skipper, J.S. Wishnok, and S.R. Tannenbaum Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids Anal Biochem 126 1982 131 138
18. G. Guillemin, F.D. Boussin, J. Croitoru, M. Franck-Duchenne, R. Le Grand, and F. Lazarini Obtention and characterization of primary astrocyte and microglial cultures from adult monkey brains J Neurosci Res 49 1997 576 591
19. C. Hao, L.J. Guilbert, and S. Fedoroff Production of colony-stimulating factor-1 (CSF-1) by mouse astroglia in vitro J Neurosci Res 27 1990 314 323
20. J.A. Hewett, S.J. Hewett, S. Winkler, and S.E. Pfeiffer Inducible nitric oxide synthase expression in cultures enriched for mature oligodendrocytes is due to microglia J Neurosci Res 56 1999 189 198
21. S.J. Hewett Interferon-gamma reduces cyclooxygenase-2-mediated prostaglandin E2 production from primary mouse astrocytes independent of nitric oxide formation J Neuroimmunol 94 1999 134 143
22. S.J. Hewett, J.A. Corbett, M.L. McDaniel, and D.W. Choi Interferon-gamma and interleukin-1 beta induce nitric oxide formation from primary mouse astrocytes Neurosci Lett 164 1993 229 232
23. R.C. Janzer The blood-brain barrier: cellular basis J Inherit Metab Dis 16 1993 639 647
24. G.R. John, S.C. Lee, X. Song, M. Rivieccio, and C.F. Brosnan IL-1-regulated responses in astrocytes: relevance to injury and recovery Glia 49 2005 161 176
25. J.K. Krady, A. Basu, S.W. Levison, and R.J. Milner Differential expression of protein tyrosine kinase genes during microglial activation Glia 40 2002 11 24
26. G.W. Kreutzberg Microglia: a sensor for pathological events in the CNS Trends Neurosci 19 1996 312 318
27. S.K. Malhotra, T.K. Shnitka, and J. Elbrink Reactive astrocytes - a review Cytobios 61 1990 133 160
28. L. Minghetti, A. Nicolini, E. Polazzi, A. Greco, M. Perretti, and L. Parente Down-regulation of microglial cyclo-oxygenase-2 and inducible nitric oxide synthase expression by lipocortin 1 Br J Pharmacol 126 1999 1307 1314
29. D.L. Montgomery Astrocytes: form, functions, and roles in disease Vet Pathol 31 1994 145 167
30. S. Murphy Production of nitric oxide by glial cells: regulation and potential roles in the CNS Glia 29 2000 1 13
31. M.K. O'Banion Cyclooxygenase-2: molecular biology, pharmacology, and neurobiology Crit Rev Neurobiol 13 1999 45 82
32. R. Parri, and V. Crunelli An astrocyte bridge from synapse to blood flow Nat Neurosci 6 2003 5 6
33. L. Santambrogio, S.L. Belyanskaya, F.R. Fischer, B. Cipriani, C.F. Brosnan, and P. Ricciardi-Castagnoli Developmental plasticity of CNS microglia Proc Natl Acad Sci USA 98 2001 6295 6300
34. M.L. Simmons, and S. Murphy Induction of nitric oxide synthase in glial cells J Neurochem 59 1992 897 905
35. K.J. Smith, and H. Lassmann The role of nitric oxide in multiple sclerosis Lancet Neurol 1 2002 232 241
36. E. Solenov, H. Watanabe, G.T. Manley, and A.S. Verkman Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method Am J Physiol Cell Physiol 286 2004 426 432
37. R.A. Swanson, J. Liu, J.W. Miller, J.D. Rothstein, K. Farrell, and B.A. Stein Neuronal regulation of glutamate transporter subtype expression in astrocytes J Neurosci 17 1997 932 940
38. B. Tedeschi, J.N. Barrett, and R.W. Keane Astrocytes produce interferon that enhances the expression of H-2 antigens on a subpopulation of brain cells J Cell Biol 102 1986 2244 2253
39. C. Thery, E.R. Stanley, and M. Mallat Interleukin 1 and tumor necrosis factor-alpha stimulate the production of colony-stimulating factor 1 by murine astrocytes J Neurochem 59 1992 1183 1186
40. D.L. Thiele, M. Kurosaka, and P.E. Lipsky Phenotype of the accessory cell necessary for mitogen-stimulated T and B cell responses in human peripheral blood: delineation by its sensitivity to the lysosomotropic agent, l-leucine methyl ester J Immunol 131 1983 2282 2290
41. J.L. Trackey, T.F. Uliasz, and S.J. Hewett SIN-1-induced cytotoxicity in mixed cortical cell culture: peroxynitrite-dependent and -independent induction of excitotoxic cell death J Neurochem 79 2001 445 455
42. T.F. Uliasz, and S.J. Hewett A microtiter trypan blue absorbance assay for the quantitative determination of excitotoxic neuronal injury in cell culture J Neurosci Methods 100 2000 157 163
43. A.S. Vidwans, T.F. Uliasz, J.A. Hewett, and S.J. Hewett Differential modulation of prostaglandin H synthase-2 by nitric oxide-related species in intact cells Biochemistry 40 2001 11533 11542
44. H. Xiong, K. Yamada, H. Jourdi, M. Kawamura, N. Takei, and D. Han Regulation of nerve growth factor release by nitric oxide through cyclic GMP pathway in cortical glial cells Mol Pharmacol 56 1999 339 347