Development of a fluorescence polarization binding assay for asialoglycoprotein receptor

Analytical Biochemistry

Volumn 425, Issue 1, 2012, Pages 43-46

  Kornilova, Anna Y ^a   Algayer, Bethany ^a   Breslin, Michael ^a   Uebele, Victor ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Binding assay; Crude receptor extract; Membrane extracts

Indexed keywords

BINDING ENERGY; FLUORESCENCE;

ASIALOGLYCOPROTEIN RECEPTORS; BINDING ASSAYS; CRUDE RECEPTOR EXTRACT; FLUORESCENCE POLARIZATION; FLUORESCENT POLARIZATION; MEMBRANE PREPARATION; MULTI STEP METHODS; OPTIMIZED CONDITIONS;

POLARIZATION;

ASIALOGLYCOPROTEIN RECEPTOR;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; FLUORESCENCE POLARIZATION; LIVER; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR BINDING;

RATTUS;

EID: 84859513481     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.02.024     Document Type: Article

References (20)

1. K.G. Rice, and Y.C. Lee Oligosaccharide valency and conformation in determining binding to the asialoglycoprotein receptor of rat hepatocytes Adv. Enzymol. Relat. Areas Mol. Biol. 66 1993 41 83
2. A. Akinc, W. Querbes, S. De, J. Qin, and M.A. Maier Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms Mol. Ther. 18 2010 1357 1364
3. D.B. Rozema, D.L. Lewis, D.H. Wakefield, S.C. Wong, J.J. Klein, P.L. Roesch, S.L. Bertin, T.W. Reppen, Q. Chu, A.V. Blokhin, J.E. Hagstrom, and J.A. Wolff Dynamic polyconjugates for targeted in vivo delivery of siRNA to hepatocytes Proc. Natl. Acad. Sci. USA 104 2007 12982 12987
4. L.A. Sliedregt, P.C. Rensen, E.T. Rump, P.J. van Santbrink, M.K. Bijsterbosch, A.R. Valentijn, G.A. van der Marel, J.H. van Boom, T.J. van Berkel, and E.A. Biessen Design and synthesis of novel amphiphilic dendritic galactosides for selective targeting of liposomes to the hepatic asialoglycoprotein receptor J. Med. Chem. 42 1999 609 618
5. C. Plank, K. Zatloukal, M. Cotten, K. Mechtler, and E. Wagner Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand Bioconjug. Chem. 3 1992 533 539
6. D.T. Connolly, R.R. Townsend, K. Kawaguchi, W.R. Bell, and Y.C. Lee Binding and endocytosis of cluster glycosides by rabbit hepatocytes: evidence for a short-circuit pathway that does not lead to degradation J. Biol. Chem. 257 1982 939 945
7. D.A. Grant, and N. Kaderbhai A reassessment of the assay for the asialoglycoprotein receptor and its use in the quantification of receptor distribution in hepatocytes Biochem. J. 234 1986 131 137
8. H. Yura, M. Ishihara, Y. Kanatani, B. Takase, H. Hattori, S. Suzuki, M. Kawakami, and T. Matsui Interaction study between synthetic glycoconjugate ligands and endocytic receptors using flow cytometry J. Biochem. 139 2006 637 643
9. R.T. Lee, M.H. Wang, W.J. Lin, and Y.C. Lee New and more efficient multivalent glyco-ligands for asialoglycoprotein receptor of mammalian hepatocytes Bioorg. Med. Chem. 19 2011 2494 2500
10. J. Hill In vitro drug metabolism using liver microsomes Curr. Protoc. Pharmacol. 2004 unit 7.8
11. R.T. Lee, and Y.C. Lee Preparation of cluster glycosides of N-acetylgalactosamine that have subnanomolar binding constants towards the mammalian hepatic Gal/GalNAc-specific receptor Glycoconjugate J. 4 1987 317 328
12. J.H. Zhang, T.D. Chung, and K.R. Oldenburg A simple statistical parameter for use in evaluation and validation of high throughput screening assays J. Biomol. Screening 4 1999 67 73
13. 3, a synthetic glycopeptide ligand for the asialoglycoprotein receptor Bioconjugate Chem. 5 1994 612 620
14. 3, a ligand with known high affinity for the asialoglycoprotein receptor Syn. Lett. 6 2000 797 800
15. R.T. Lee, and Y.C. Lee Preparation of a high-affinity photolabeling reagent for the Gal/GalNAc lectin of mammalian liver: demonstration of galactose-combining sites on each subunit of rabbit hepatic lectin Biochemistry 25 1986 6835 6841
16. Y. Wu, W.T. Jiaang, K.G. Lin, C.M. Huang, C.H. Chang, Y.L. Sun, K.H. Fan, W.C. Hsu, H.E. Wang, S.B. Lin, and S.T. Chen A new N-acetylgalactosamine containing peptide as a targeting vehicle for mammalian hepatocytes via asialoglycoprotein receptor endocytosis Curr. Drug Delivery 1 2004 119 127
17. A. Harris, S. Cox, D. Burns, and C. Norey Miniaturization of fluorescence polarization receptor-binding assays using CyDye-labeled ligands J. Biomol. Screening 8 2003 410 420
18. National Institues of Health Chemical Genomics Center, http://www.assay.nih.gov/assay/index.php/Table-of-Contents.
19. S. Lin, C.L. Bock, D.B. Gardner, J.C. Webster, M.F. Favata, J.M. Trzaskos, and K.R. Oldenburg A high-throughput fluorescent polarization assay for nuclear receptor binding utilizing crude receptor extract Anal. Biochem. 300 2002 15 21
20. R.J. Stockert The asialoglycoprotein receptor: relationships between structure, function, and expression Physiol. Rev. 75 1995 591 609