Exploring the 5'-UTR DNA region as a target for optimizing recombinant gene expression from the strong and inducible Pm promoter in Escherichia coli

Journal of Biotechnology

Volumn 158, Issue 4, 2012, Pages 224-230

  Berg, Laila ^a   Kucharova, Veronika ^a   Bakke, Ingrid ^a   Valla, Svein ^a   Brautaset, Trygve ^b  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b SINTEF MATERIALS AND CHEMISTRY   (Norway)

Author keywords

Combinatorial mutagenesis; Interferon 2b; Pm xylS; Recombinant gene expression; Synthetic operon

Indexed keywords

APPLIED POTENTIALS; CONTEXT DEPENDENCY; CYTOKINES; EXPRESSION LEVELS; GENE SEQUENCES; HIGH LEVEL EXPRESSION; LACTAMASES; MUTANT LIBRARIES; PHOSPHOGLUCOMUTASE; PRODUCTION LEVEL; RECOMBINANT GENE EXPRESSIONS; STIMULATORY EFFECTS; SYNTHETIC OPERON; UNTRANSLATED REGIONS; WILD TYPES;

ANTIBIOTICS; DNA; ENCODING (SYMBOLS); ESCHERICHIA COLI; GLYCOPROTEINS; OPTIMIZATION;

GENE EXPRESSION;

5' UNTRANSLATED REGION; ARTICLE; BACTERIAL GENE; CELB GENE; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; GENE SEQUENCE; GENETIC VARIABILITY; INF A2B GENE; NONHUMAN; OPERON; PM GENE PROMOTER; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION;

5' UNTRANSLATED REGIONS; BETA-LACTAMASES; DNA; DNA, RECOMBINANT; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; INTERFERON-ALPHA; MUTATION; OPERON; PHOSPHOGLUCOMUTASE; PROMOTER REGIONS, GENETIC; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI;

EID: 84859509809     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.07.012     Document Type: Article

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