The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli

Applied and Environmental Microbiology

Volumn 73, Issue 3, 2007, Pages 906-912

  Sletta, H ^a   Tondervik A ^b   Hakvag S ^b   Vee Aune, T E ^b   Nedal, A ^b   Aune, R ^a   Evensen, G ^c   Valla, S ^b   Ellingsen, T E ^a   Brautaset, T ^a  

^a SINTEF MATERIALS AND CHEMISTRY   (Norway)

^b NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^c Alpharma AS   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCOMMUNICATIONS; CELL CULTURE; GENES; INTERFERONS; MOLECULAR BIOLOGY; PROTEINS;

CELL DENSITY; COLONY STIMULATING FACTOR (CSF); GRANULOCYTES; TRANSLOCATION;

ESCHERICHIA COLI;

ALPHA2B INTERFERON; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY; COLIFORM BACTERIUM; GENE EXPRESSION; OPTIMIZATION; PROTEIN; SECRETION; SIGNAL; TRANSLOCATION;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL DENSITY; ESCHERICHIA COLI; GENE FUSION; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REACTION OPTIMIZATION; SIGNAL TRANSDUCTION; VOLUMETRY;

BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; BIOTECHNOLOGY; CULTURE MEDIA; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; IMMUNOGLOBULIN FRAGMENTS; INTERFERON ALFA-2B; MOLECULAR SEQUENCE DATA; OXAZOLES; PLASMIDS; POLYSACCHARIDE-LYASES; PROTEIN SORTING SIGNALS; RECOMBINATION, GENETIC;

ESCHERICHIA COLI;

EID: 33846938509     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01804-06     Document Type: Article

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