Novel fluorescent antagonist as a molecular probe in A 3 adenosine receptor binding assays using flow cytometry

Biochemical Pharmacology

Volumn 83, Issue 11, 2012, Pages 1552-1561

  Kozma, Eszter ^a   Kumar, T Santhosh ^a   Federico, Stephanie ^b   Phan, Khai ^a   Balasubramanian, Ramachandran ^a   Gao, Zhan Guo ^a   Paoletta, Silvia ^c   Moro, Stefano ^c   Spalluto, Giampiero ^b   Jacobson, Kenneth A ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b UNIVERSITY OF TRIESTE   (Italy)

^c UNIVERSITY OF PADOVA   (Italy)

Author keywords

A 3 adenosine receptor; Flow cytometry; Fluorescence; G protein coupled receptor; Purines

Indexed keywords

2 (6 AMINO 3 IMINO 4,5 DISULFONATO 3H XANTHEN 9 YL) 5 [[6 [4 [4 [[9 CHLORO 2 (FURAN 2 YL)[1,2,4]TRIAZOLO[1,5 C]QUINAZOLIN 5 YL]AMINO] 4 OXOBUTYL] 1H 1,2,3 TRIAZOL 1 YL]HEXYL]CARBAMOYL]BENZOATE; 4 AMINO 3 IODOBENZYL 5' N METHYLCARBOXAMIDOADENOSINE I 125; ADENOSINE A3 RECEPTOR; ADENOSINE A3 RECEPTOR AGONIST; ADENOSINE A3 RECEPTOR ANTAGONIST; CELL MEMBRANE PROTEIN; CYCLIC AMP; FLUORESCENT DYE; MRS 5449; PYRAZOLO[4,3 E][1,2,4]TRIAZOLO[1,5 C]PYRIMIDIN 5 YLAMINE; RADIOLIGAND; TRIAZOLO[1,5 C]QUINAZOLIN 5 YLAMINE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; BINDING ASSAY; CHO CELL; CONCENTRATION RESPONSE; CONJUGATION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; INHIBITION KINETICS; LIGAND BINDING; MOLECULAR DOCKING; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR BINDING;

ADENOSINE A3 RECEPTOR ANTAGONISTS; ANIMALS; CHO CELLS; CRICETINAE; FLOW CYTOMETRY; FLUORESCENCE; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR STRUCTURE; PROTEIN BINDING; PROTEIN CONFORMATION; PYRAZOLES; PYRIDINES; QUINAZOLINES; RECEPTOR, ADENOSINE A3; STRUCTURE-ACTIVITY RELATIONSHIP; TRIAZOLES;

EID: 84859505693     PISSN: 00062952     EISSN: 18732968     Source Type: Journal    
DOI: 10.1016/j.bcp.2012.02.019     Document Type: Article

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